Effects of CO₂Lasers on Dental Pulp Biology in Rats

Abstract

These results demonstrate that CO2 laser irradiation induces degeneration in the pulp tissue, which is then repaired by newly formed odontoblast-like cells.

Histopathological findings of an exposed human pulp carbonised by CO2 laser irradiation: A case report

“…Apart from dentine bridge formation, teeth from approximately 30 days post treatment in both groups showed irregular matrix formation close to the dentine walls, which seemed to be generated by the stimulation of laser energy (13)(14)(15). It is suggested that if an appropriate amount of stimulation by laser energy is applied to exposed pulp tissue, pulpal healing and repair may be enhanced.…”

“…The laser-irradiated pulp tissue exhibits various responses, which largely depend on irradiation settings such as wavelength, output power, waveform, pulse duration, interval time, focal spot size, energy density, total energy and others (2)(3)(4)(5)(6)(7)15,(20)(21)(22). Therefore, it is imperative to understand the precise specifications of laser devices when using a laser beam for treatments in the clinic.…”

“…Laser irradiation also has an enhancing effect on the healing process of injured tissue by promoting pain attenuation, nerve repair, angiogenesis, odontogenic differentiation and mineralisation within the dental pulp . Moreover, CO 2 laser irradiation has the potential to increase the expression of various proteins associated with hard tissue formation, such as dentine matrix protein and dentine sialophosphoprotein, and promote immune functions for cell differentiation and proliferation in wound healing processes . However, although teeth capped with SE exhibited extracellular matrix formation at the exposed surface, definite dentine bridge formation was not observed even after 261 days post treatment.…”

Effect of EDTA-treated dentin on the differentiation of mouse iPS cells into osteogenic/odontogenic lineages <i>in vitro</i> and <i>in vivo </i>

“…The protocol for qRT-PCR was performed according to Ser-Od et al 20) . Total RNA was obtained from each dish using the acid guanidium thiocyanate/phenol-chloroform method.…”