Effects of ClpP protease on biofilm formation of Enterococcus faecalis

Feng, Ying; Wang, Hongyuan; Lü, Hao; Liu, Yi; Lv, Hong

doi:10.1590/1678-7757-2020-0733

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…Because a variety of bacterial species were presented in the oral cavity, microorganisms and their by-products are associated with polymicrobial EIs [ 2 , 3 ]. Particularly, Enterococcus faecalis ( E. faecalis ) related biofilms are covered in the root canals and invade into dentinal tubules, which may result in persistent infection and refractory periapical periodontitis [ 4 , 5 ]. Meanwhile, because of the complex root canal system (e.g., dentinal tubules, isthmuses, the apical delta, etc.…”

Section: Introductionmentioning

confidence: 99%

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

et al. 2022

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Enterococcus faecalis (E. faecalis) biofilm-associated persistent endodontic infections (PEIs) are one of the most common tooth lesions, causing chronic periapical periodontitis, root resorption, and even tooth loss. Clinical root canal disinfectants have the risk of damaging soft tissues (e.g., mucosa and tongue) and teeth in the oral cavity, unsatisfactory to the therapy of PEIs. Nanomaterials with remarkable antibacterial properties and good biocompatibility have been developed as a promising strategy for removing pathogenic bacteria and related biofilm. Herein, carbon dots (CDs) derived from fucoidan (FD) are prepared through a one-pot hydrothermal method for the treatment of PEIs. The prepared FDCDs (7.15 nm) with sulfate groups and fluorescence property are well dispersed and stable in water. Further, it is found that in vitro FDCDs display excellent inhibiting effects on E. faecalis and its biofilm by inducing the formation of intracellular and extracellular reactive oxygen species and altering bacterial permeability. Importantly, the FDCDs penetrated the root canals and dentinal tubules, removing located E. faecalis biofilm. Moreover, the cellular assays show that the developed FDCDs have satisfactory cytocompatibility and promote macrophage recruitment. Thus, the developed FDCDs hold great potential for the management of PEIs. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

et al. 2022

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“…ClpP has been reported to play roles in stress tolerance, antimicrobial tolerance, and biofilm formation of Ef (Zheng et al 2020 ). Feng Y et al reported ClpP deletion strains inhibiting Ef’s survival and biofilm-forming (Feng et al 2021 ). PerR, a regulator of oxidative-stress response, could affect the virulence and resistance of Ef to hydrogen peroxide challenge (Verneuil et al 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects

Zhou,

Yuan,

Yang

et al. 2024

Appl Microbiol Biotechnol

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To investigate the cell-cell interactions of intergeneric bacterial species, the study detected the survival of Enterococcus faecalis (Ef) under monospecies or coaggregation state with Fusobacterium nucleatum subsp. polymorphum (Fnp) in environmental stress. Ef and Fnp infected the human macrophages with different forms (Ef and Fnp monospecies, Ef-Fnp coaggregates, Ef + Fnp cocultures) for exploring the immunoregulatory effects and the relevant molecular mechanisms. Meanwhile, the transcriptomic profiles of coaggregated Ef and Fnp were analyzed. Ef was shown to coaggregate with Fnp strongly in CAB within 90 min by forming multiplexes clumps. Coaggregation with Fnp reinforced Ef resistance against unfavorable conditions including alkaline, hypertonic, nutrient-starvation, and antibiotic challenges. Compared with monospecies and coculture species, the coaggregation of Ef and Fnp significantly facilitates both species to invade dTHP-1 cells and aid Ef to survive within the cells. Compared with coculture species, dual-species interaction of Ef and Fnp significantly decreased the levels of pro-inflammatory cytokines IL-6, TNF-α, and chemokines MCP-1 secreted by dTHP-1 cells and lessened the phosphorylation of p38, JNK, and p65 signaling pathways. The transcriptome sequencing results showed that 111 genes were differentially expressed or Ef-Fnp coaggregated species compared to Ef monospecies; 651 genes were differentially expressed for Fnp when coaggregation with Ef. The analysis of KEGG pathway showed that Ef differentially expressed genes (DEGs) were enriched in quorum sensing and arginine biosynthesis pathway; Fnp DEGs were differentially concentrated in lipopolysaccharide (LPS) biosynthesis, biofilm formation, and lysine degradation pathway compared to monospecies. Key points • Coaggregated with Fnp aids Ef’s survival in environmental stress, especially in root canals after endodontic treatment. • The coaggregation of Ef and Fnp may weaken the pro-inflammatory response and facilitate Ef to evade killed by macrophages. • The coaggregation between Ef and Fnp altered interspecies transcriptional profiles.

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“…The absence of ClpP protease induces alterations in the growth pattern of Enterococcus faecalis and diminishes the polysaccharide matrix, thereby impairing its ability to form biofilms. This suggests a potential therapeutic avenue targeting ClpP protease for the treatment of Enterococcus faecalis infections ( Feng et al., 2021 ). Furthermore, recent research by Mabanglo et al.…”

Section: Mechanisms Targeting Enterococcus Faecalis ...mentioning

confidence: 99%

Strategies and mechanisms targeting Enterococcus faecalis biofilms associated with endodontic infections: a comprehensive review

Yang,

Meng,

Zhen

et al. 2024

Front. Cell. Infect. Microbiol.

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Enterococcus faecalis is one of the main microorganisms that infects root canals, ranking among the most prevalent microorganisms associated with endodontic treatment failure. Given its pervasive presence in persistent endodontic infections, the successful elimination of Enterococcus faecalis is crucial for effective endodontic treatment and retreatment. Furthermore, Enterococcus faecalis can form biofilms - defense structures that microbes use to fight environmental threats. These biofilms confer resistance against host immune system attacks and antibiotic interventions. Consequently, the presence of biofilms poses a significant challenge in the complete eradication of Enterococcus faecalis and its associated disease. In response, numerous scholars have discovered promising outcomes in addressing Enterococcus faecalis biofilms within root canals and undertaken endeavors to explore more efficacious approaches in combating these biofilms. This study provides a comprehensive review of strategies and mechanisms for the removal of Enterococcus faecalis biofilms.

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Effects of ClpP protease on biofilm formation of Enterococcus faecalis

Cited by 9 publications

References 33 publications

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects

Strategies and mechanisms targeting Enterococcus faecalis biofilms associated with endodontic infections: a comprehensive review

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