Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells

Jin, Zhang; Hu, Dakang; Wang, Dongguo; Liu, Yang; Liu, Chibo; Yu, Lei; Qu, Ying; Luo, Xinhua; Yang, Jing; Yu, Jiangnan; Liu, Shuangchun; Li, Xiangyang

doi:10.3892/mmr.2013.1688

Cited by 5 publications

(14 citation statements)

References 22 publications

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“…The expression of cpsA is the basis of the pathogenicity of SP and ply expression is more important than that of psaA in SP invasion into the blood system. Our current data demonstrated that inflammation induction of different SP sources tends to be consistent [ 20 ]. However, the infection types are determined mainly by different sources of SP itself, especially its expression of virulence genes.…”

Section: Discussionmentioning

confidence: 60%

“…Thus, Ply toxin has a wide range of biological activities, such as facilitation of S. pneumoniae colonization and pathogenicity. Ply can also activate the classic complement pathway in the human body, inhibit cough and bactericidal activity and migration of white blood cells [ 16 ], stimulate IL-8 synthesis [ 17 ], and increase ICAM-1 and IL-1β secretions [ 18 - 20 ]. The Ply impact on the immune response to the pneumococcus is highly dependent on the strain background, thus, it is surely important of the interaction between specific virulence factors and other components of the genetic background of this organism [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

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In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes

Liu

et al. 2015

Eur J Med Res

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BackgroundStreptococcus pneumoniae is one major cause of pneumonia in human and contains various virulence factors that contribute to pathogenesis of pneumococcal disease. This study investigated the role of pneumolysin, Ply, in facilitating S. pneumoniae invasion into the host blood stream.MethodsS. pneumoniae strains were isolated from clinical blood and sputum samples and confirmed by PCR. Expression of ply gene was assessed by infecting human monocytes and pneumocytes.ResultsA total of 23 strains of S. pneumoniae isolated from blood (n = 11) and sputum (n = 12) along with S. pneumoniae ATCC49619 were used to infect human monocyte (THP-1) and Type II pneumocyte (A549) cell lines. All clinical strains of S. pneumoniae showed higher expression of ply mRNA than the American Type Culture Collection (ATCC) strain. Among the clinical strains, blood isolates showed higher expression of ply genes than sputum isolates, i.e., 21.5- to 21.6-folds in THP-1 and 20.4- to 24.9-folds in A549 cell lines.ConclusionsThe data from the current study demonstrated that ply gene of blood- and sputum-derived S. pneumoniae is differentially expressed in two different cell lines. Under survival pressure, ply is highly expressed in these two cell lines for blood-derived S. pneumoniae, indicating that ply gene may facilitate S. pneumoniae invasion into the host blood system.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes

Liu

et al. 2015

Eur J Med Res

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“…IL-8 is a multifunctional proinflammatory cytokine that can be secreted by numerous cells such as mesothelial cells, tumor cells, neutrophils, endothelial cells, and monocytes (8,16,17). The main role of IL-8 during inflammatory response is the chemotaxis of immune cells to the site of inflammation, which triggers the release of inflammatory mediators (18,19).…”

Section: Discussionmentioning

confidence: 99%

“…There are various components associated with S. pneumoniae-induced immune response, such as the capsule and other virulence factors (5)(6)(7). In addition, many immune cells such as neutrophils, monocytes/macrophages, and dendritic cells are involved in this process (6,(8)(9)(10), which are responsible for the release of factors including IL-1α, IL-1β, IL-6, IFN-α, IL-8, and ICAM-1 (6,8).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Impact of Streptococcus pneumoniae on A549 Pneumocytes

Liu

et al. 2019

Jundishapur J Microbiol

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Background: Streptococcus pneumoniae is a leading pathogen worldwide and its pathogenesis remains unclear. Objectives: The study aimed to investigate the secretion of interleukin-8 (IL-8) and soluble intercellular adhesion molecule-1 (ICAM-1) from A549 pneumocytes stimulated by different S. pneumoniae strains and the mechanism of blood-derived S. pneumoniae (bd-SP) in invading the blood system. Methods: Twenty-three clinical strains of S. pneumoniae isolated in 2009 along with ATCC49619 were cultured and suspended in 2018. Then, A549 pneumocytes were resuspended after culture and collection, and inoculated into culture plates. Streptococcus pneumoniae suspensions were then inoculated. Normal saline was blank control. The plates were incubated for four and eight hours. Then, the suspensions were collected and centrifuged. The supernatant was analyzed for IL-8 and ICAM-1 by ELISA. Results:The concentrations of IL-8 were 180.6, 188.5, 223.4 ± 19.9, and 230.3 ± 38.6 pg/mL for blank control, ATCC49619, blood-derived S. pneumoniae (bd-SP) and sputum-derived S. pneumoniae (sd-SP) at four-hour stimulation, respectively, and 249.2, 275.7, 224.0 ± 27.8, and 242.3 ± 33.1 pg/mL at eight-hour stimulation. The concentrations of ICAM-1 were 14.8, 12.1, 19.9 ± 17.2, and 26.1 ± 28.6 ng/mL for blank control, ATCC49619, bd-SP, and sd-SP at 4 hours, respectively, and 32.6, 150.8, 69.4 ± 45.1, and 58.7 ± 30.1 ng/mL at 8 hours. Conclusions:Streptococcus pneumoniae could upregulate the secretion of IL-8 and ICAM-1 from A549 pneumocytes. No significant difference was found between bd-SP and sd-SP which was in contrast to what was found between clinical S. pneumoniae and ATCC49619. Host response may not be a vital factor for different S. pneumoniae infections.

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“…ATCC49619 is a standard S pneumoniae strain, which is less virulent than clinical ones as shown in our previous study. 8,9,33 The intriguing difference among clinical S pneumoniae groups lies in sICAM-1 except IL-1β, IL-6, IL-10, and IL-8.…”

Section: Il-8 Could Promote Inflammatory Cells' Accumulation and Actimentioning

confidence: 99%

Impact of different Streptococcus pneumoniae on the secretion of interleukin and adhesin from THP‐1 monocytes

Ren

Zhang

et al. 2019

Clinical Laboratory Analysis

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BackgroundTo investigate the secretion of interleukin‐1β (IL‐1β), IL‐6, IL‐10, IL‐8, and soluble intercellular adhesin molecule 1 (sICAM‐1) from THP‐1 monocytes stimulated by different Streptococcus pneumoniae (S pneumoniae) strains.MethodsFifty‐eight strains of S pneumoniae were collected: ATCC49619, 23 from sputum (sd‐SP), 23 from blood (bd‐SP), and 11 from cerebrospinal fluid (CSF; cd‐SP). Such strains were cultured and suspended at 0.5 McFarland. THP‐1 monocytes were cultured and resuspended at 5.0 × 108/L, which were stimulated by S pneumoniae for 4, 8, and 12 hours, respectively. The suspensions were analyzed for IL‐1β, IL‐6, IL‐10, IL‐8, and sICAM‐1 using an ELISA method. The data were assayed with SPSS 19.0.ResultsContrary to IL‐10, the concentrations of IL‐1β, IL‐6, IL‐8, and sICAM‐1 all increased first and then decreased. IL‐1β and sICAM‐1 levels in the ATCC49619 group were both higher than all the clinical S pneumoniae groups (sd‐SP, bd‐SP, and cd‐SP), IL‐6 and IL‐8 versa, and IL‐10 equal. The difference among clinical S pneumoniae groups lay only in sICAM‐1. cd‐SP group showed lower sICAM‐1 concentrations than sd‐SP and bd‐SP groups at both 4 and 8 hours. However, they became equal at 12 hours.ConclusionsThe secretion summit is 8 hours for IL‐1β, IL‐6, IL‐8, and sICAM‐1, bottom for IL‐10. Different clinical S pneumoniae strains show similar ability to induce THP‐1 cells secreting interleukins. However, cd‐SP induces THP‐1 cells secreting lower sICAM‐1 than sd‐SP and bd‐SP, which may in turn facilitate its invasion into CSF.

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Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells

Cited by 5 publications

References 22 publications

In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes

In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes

Investigation of the Impact of Streptococcus pneumoniae on A549 Pneumocytes

Impact of different Streptococcus pneumoniae on the secretion of interleukin and adhesin from THP‐1 monocytes

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