Effects of Citrus Flavonoids Against Microvascular Damage Induced by Hypoperfusion and Reperfusion in Rat Pial Circulation

Mastantuono, Teresa; Battiloro, Laura; Sabatino, Lina; Chiurazzi, Martina; Maro, Martina Di; Muscariello, Espedita; Colantuoni, Antonio; Lapi, Dominga

doi:10.1111/micc.12207

Cited by 19 publications

(19 citation statements)

References 38 publications

(39 reference statements)

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“…API-induced vasorelaxation was significantly attenuated by nitric oxide synthase inhibitor L-NAME but not by cyclooxygenase inhibitor indomethacin, suggesting that nitric oxide production but not prostanoid production is involved in the vasorelaxation. This is consistent with the reported results that API vasorelaxation in rat pial arteries was attenuated by inhibition of nitric oxide synthase [14] and that API enhanced rat aortic endothelial nitric oxide synthase activity and endothelial nitric oxide synthesis [6]. API relaxation was also reduced by Cl - deprivation, NFA, CaCC inh -A01and 4-AP, suggesting that chloride channels, Kv channels may be involved in the relaxation.…”

Section: Discussionsupporting

confidence: 92%

“…Apigenin (API, C 15 H 10 O 5 , MW270.24), a natural flavonoid present in a lot of edible plants such as celery, parsley and oranges, possesses significant pharmacological activities and has been suggested to treat a variety of disorders such as cancers [1], neurodegeneration [2], metabolic syndrome [3], hyperlipidemia [4], vasodilative impairments [5-8] and vascular restenosis [9]. API-induced vasodilation was demonstrated in rat aorta [10-13], pial artery [14] and mesenteric artery [15]. The suggested mechanisms underlying its vasodilation include production of nitric oxide and guanosine 3’, 5’-cyclic monophosphate, activation of transient receptor potential vanilloid 4 cation channel, calcium-activated potassium channels (K Ca ) and ATP-sensitive potassium channels (K ATP ), inhibition of extracellular Ca 2+ influx.…”

Section: Introductionmentioning

confidence: 99%

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Apigenin relaxes rat intrarenal arteries: involvement of Cl⁻ channels and K⁺ channels

Jing

Dong

et al. 2018

Preprint

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27The vasodilator effect of apigenin (API) was demonstrated in a number of vascular beds. We 28 aimed to characterize the vasospasmolytic and electrophysiological effects of apigenin (API) in 29 intrarenal arteries (IRAs). The vascular tone of male rat isolated IRAs was recorded with a 30 myograph. Transmembrane Clcurrents through Ca 2+ -activated Clchannels (CaCCs), K + currents 31 through voltage-gated K + (Kv) channels and inwardly rectifier K + (Kir) channels were recorded 32 with patch clamp in the freshly isolated arterial smooth muscle cells (ASMCs). Preincubation with 33 API (10-100 μM) concentration-dependently depressed the contractions induced by KCl, 34 9,11-dideoxy-9α,11α-methanoepoxy prostaglandin F 2α (U46619), phenylephrine and vasopressin 35 without significant preference and the IC 50 values were 13.27-26.26 μM. Acute application of API 36 elicited instant relaxations in the IRAs precontracted with these vasoconstrictors and the RC 50 37 values were 5.80-24.33 μM. API relaxation was attenuated by chloride deprivation, CaCC 38 blockers, Kv blocker and nitric oxide synthase inhibitor, but not by Kir blocker and 39 cyclooxygenase inhibitor. At 10-100 μM, API depressed CaCC currents and Kir currents while 40 enhanced Kv currents of IRA ASMCs. The present results demonstrate that API counteracts 41 various vasoconstrictors noncompetitively and nonspecifically and suggest that modulation of 42 CaCCs, Kv and Kir channels of IRA ASMCs is involved in its vasospasmolytic effects. 43 44

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Apigenin relaxes rat intrarenal arteries: involvement of Cl⁻ channels and K⁺ channels

Jing

Dong

et al. 2018

Preprint

View full text Add to dashboard Cite

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“…Macrophage-derived foam cells were prepared as previously described (18). THP-1 cells were cultured in RPMI-1640 containing 10% FBS.…”

Section: Methodsmentioning

confidence: 99%

Apigenin in the regulation of cholesterol metabolism and protection of blood vessels

Zhang

Song

et al. 2017

Experimental and Therapeutic Medicine

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Hyperlipidemia is a major independent risk factor for atherosclerosis. Seeking natural compounds in medicinal plants capable of reducing blood fat and studying their mechanisms of action has been the focus of research in recent years. The aim of the present study was to analyze the mechanisms of apigenin in regulating cholesterol metabolism and protecting blood vessels, and to provide a theoretical basis for the clinical application of apigenin. The mouse model of hyperlipidemia was established to verify the efficacy of apigenin in improving hyperlipidemia and to observe the mechanism of action of apigenin in reducing cholesterol content. In vitro cell experiments were conducted to evaluate the role of apigenin in mediating reverse cholesterol transport. Additionally, H2O2-injured human umbilical venous endothelial cells (EA.hy926 cells) were used for further study on the roles of apigenin in resisting oxidization and protecting vascular endothelial cells. Apigenin significantly regulated blood fat, reduced animal weight, and reduced total cholesterol (P=0.024), triglyceride (P=0.031) and low-density lipoprotein cholesterol (P=0.014) in the serum of the high-fat diet mice. Apigenin improved the blood lipid metabolism of the hyper-lipidemia model mice. Body weight and serum cholesterol content increased abnormally (P=0.003) as a consequence of high-fat diet. Apigenin increased the activity of superoxide dismutase in EA.hy926 cells (P=0.043) and increased the amount of nitric oxide secreted by the cells (P=0.038). Apigenin also inhibited the proliferation of vascular smooth muscle cells in a dose-dependent manner (P=0.036). In conclusion, apigenin can regulate cholesterol metabolism in vivo and plays a role in reducing the level of blood fat by promoting cholesterol absorption and conversion, and accelerating reverse cholesterol transport. Apigenin also has a role in resisting oxidization and protecting blood vessels.

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“…Significant reduction in extravasation of EB in brain tissue and also brain water content was observed in ischaemic rats following the administration of OLE (Mohagheghi et al 2011). In a separate study, oleuropein reversed the bilateral common carotid artery occlusion and reperfusion-induced decrease in arteriolar diameter, increase in microvascular leakage and leukocyte adhesion to venules and reduction in capillary perfusion (Mastantuono et al 2015). Thus, oleuropein is potentially a promising candidate to reduce secondary injury to the brain as a result of stroke.…”

Section: Neuroprotective Effects Of Olive In Ischemia-reperfusion (Stmentioning

confidence: 90%

Olive and Its Phenolic Compound as the Promising Neuroprotective Agent

Kamil

Kumar

Yazid

et al. 2018

JSM

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Recent progress in alternative medicine has highlighted the benefits of olive as an integral part of therapeutic diet to promote healthy living. Among the thirty different phenolic compounds of olive known to date; oleocanthal, oleuropein, tyrosol and hydroxytyrosol are being increasingly investigated for their potential in prevention and healing of several major forms of neurological dysfunctions and disorders. A considerable amount of literature suggests the neuroprotective effects of olive and its phenolic compounds are owing to their roles as anti-oxidant, anti-inflammatory and anti-apoptotic agents. At preclinical level, olive attenuated cognitive dysfunctions and the functional outcomes in spinal cord injury, delayed the progression of amyloid beta pathology, improved motor and mitochondrial dysfunctions in Parkinson's disease, reversed diabetic-related neurological complications and also ameliorated cerebral pathologies in stroke. In this paper, we aim to review the neuroprotective role of olive and its phenolic derivatives in the following diseases or deficits of the nervous system that include cognitive dysfunction, neurodegenerative diseases, stroke, peripheral neuropathy and spinal cord injury.

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Effects of Citrus Flavonoids Against Microvascular Damage Induced by Hypoperfusion and Reperfusion in Rat Pial Circulation

Abstract: Hesperidin, diosmin, and apigenin showed dose-related protective effects on hypoperfusion-reperfusion injury, causing nitric oxide release and attenuating tissue edema and leukocyte adhesion.

Cited by 19 publications

References 38 publications

Apigenin relaxes rat intrarenal arteries: involvement of Cl⁻ channels and K⁺ channels

Apigenin relaxes rat intrarenal arteries: involvement of Cl⁻ channels and K⁺ channels

Apigenin in the regulation of cholesterol metabolism and protection of blood vessels

Olive and Its Phenolic Compound as the Promising Neuroprotective Agent

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Effects of Citrus Flavonoids Against Microvascular Damage Induced by Hypoperfusion and Reperfusion in Rat Pial Circulation

Abstract: Hesperidin, diosmin, and apigenin showed dose-related protective effects on hypoperfusion-reperfusion injury, causing nitric oxide release and attenuating tissue edema and leukocyte adhesion.

Cited by 19 publications

References 38 publications

Apigenin relaxes rat intrarenal arteries: involvement of Cl− channels and K+ channels

Apigenin relaxes rat intrarenal arteries: involvement of Cl− channels and K+ channels

Apigenin in the regulation of cholesterol metabolism and protection of blood vessels

Olive and Its Phenolic Compound as the Promising Neuroprotective Agent

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Product

Resources

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Apigenin relaxes rat intrarenal arteries: involvement of Cl⁻ channels and K⁺ channels

Apigenin relaxes rat intrarenal arteries: involvement of Cl⁻ channels and K⁺ channels