Effects of citric acid and phytase supplementation on nutrient digestibility and growth performance of Cirrhinus mrigala fingerlings fed on corn gluten (30%) meal based diets

Abstract

This research work was conducted in specially designed fecal collecting V-shaped tanks having capacity of 70 liters to determine the synergistic effects of citric acid (CA) and phytase enzyme on the nutrient digestibility of Cirrhinus mrigala fingerlings fed on corn gluten (30%) meal based diets. Nine test diets along with reference were prepared with phytase of 0, 500 and 1000 FTU kg‫)¹-‬ and CA 0g (0%), 75g (2.5%) and 150g (5%).Fingerlings were fed twice daily up to 5% of their body weights and fecal material was collected regularly for chemical analysis. Results of present work go in favor of diets supplemented with phytase and CA. Current research clearly hypothesized that phytase enzyme along with CA enhanced nutrient digestibility of Cirrhinus mrigala fingerlings fed on plant based diet. It was observed that maximum nutrient digestibility percentage dry matter 29%, crude protein 69%, crude fat 73% and gross energy is 70% shown by fingerlings fed on diet having phytase 500 FTU kg‫¹-‬ combined with CA 5%. In conclusion Cirrhinus mrigala fingerlings fed on diet having phytase 500 FTU kg‫¹-‬ combined with CA 5% release nutrients from phytate complexes in plant based diets thus enhance nutrient digestibility, growth rate and release less nutrients through feces thus reduce chances of aquatic pollution.

Use of phytase and citric acid supplementation on growth performance and nutrient digestibility of Cirrhinus mrigala fingerlings fed on canola meal based diet

“…A recent study conducted by Fadhal and Mustafa (2020) confirmed the role of phytase as a growth promoting dietary additive when used at 4000IU/kg in the diet of common carp. Similar results of optimized growth were reported by Hussain et al (2015) but the levels were different from our study. They recorded that supplementation of 5% CA and 500 (FTU kg -1 ) PHY resulted in the maximum growth (WG%: 401% and FCR: 1.21) of C. mrigala fingerlings fed canola meal-based diet.…”

“…Variation in the growth parameters of C. mrigala fingerlings was estimated at the beginning and end of the experiment by following standard methods (Hussain et al, 2015) (Equations 1-3). (2)…”

Synergistic Effects of Citric Acid and Phytase Supplemented Canola Meal Based Diet on Growth Performance and Nutrient Digestibility of Cyprinus Carpio Fingerlings

“…In another feeding trial, C. mrigala fingerlings showed maximum improvement of growth parameters (WG% 401% and FCR 1.21) when fed different levels of CA and PHY (i.e. 5% and 500 FTU kg -1 ) added corn gluten meal-based diet (Hussain et al, 2015). Different studies suggested that for improving growth parameters, incorporation of PHY in fish feed should be ranged between 250-1500 FTUkg -1 levels (Cao et al, 2007) while CA between 2.5-5% (Khajepour et al, 2012).…”

“…This replacement level of fish meal by plant meals along with CA and PHY supplementation proved beneficial in improving morphometric parameters of fish. In another study, acidified and PHY treated corn gluten (30%) meal-based diet was fed to C. mrigala fingerlings that resulted in increased growth performance at 5% CA and 500 FTU kg -1 level of PHY supplementation (Hussain et al, 2015). Similar to this study, the best FCR value (1.03) was found in C. mrigala fingerlings fed PHY (1000 FTU kg -1 ) and CA (2.5%) supplemented guar meal-based diets, which was significantly different (p≤0.05) from all other treatments (Hussain et al, 2020).…”

“…Canola meal being a good source of essential nutrients, proteins and fat, also contains anti nutritional factors. Phytate is also present in all plant protein-based diets, and it can affect fish nutrient digestibility (Hussain et al, 2015). Furthermore, it can bind with important amino acids in several fish species, reducing nutrient digestibility, particularly protein digestibility (Usmani and Jafri, 2002).…”