Effects of Cisplatin and its Analogues on the Permeabilityof Human Erythrocyte Membrane

Lu, Jian-min; Wang, K.; Sun, Xiaohong; Xing, Fei; An, Polin; Yang, Zhenhong; Yin, Jun‐Jie

doi:10.1155/mbd.1995.73

Cited by 9 publications

(5 citation statements)

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“…Overall literature data highlight that cisplatin interacts with lipid bilayers but its effect are complex and dependent on a variety of extrinsic factors, including pH and chloride concentration, as well as, membrane composition and other unknown factors (Mann et al, 1990; Lu et al, 1995a; Liang and Huang, 2002; Jensen and Nerdal, 2008). In fact, the importance of the physical state of membranes for the mechanisms of cisplatin and other platinum(II) complexes interaction remains to be determined.…”

Section: Cisplatin-membrane Interactionsmentioning

confidence: 99%

Cisplatin-Membrane Interactions and Their Influence on Platinum Complexes Activity and Toxicity

et al. 2019

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Cisplatin and other platinum(II) analogs are widely used in clinical practice as anti-cancer drugs for a wide range of tumors. The primary mechanism by which they exert their action is through the formation of adducts with genomic DNA. However, multiple cellular targets by platinum(II) complexes have been described. In particular, the early events occurring at the plasma membrane (PM), i.e., platinum-membrane interactions seem to be involved in the uptake, cytotoxicity and cell-resistance to cisplatin. In fact, PM influences signaling events, and cisplatin-induced changes on membrane organization and fluidity were shown to activate apoptotic pathways. This review critically discusses the sequence of events caused by lipid membrane-platinum interactions, with emphasis on the mechanisms that lead to changes in the biophysical properties of the membranes (e.g., fluidity and permeability), and how these correlate with sensitivity and resistance phenotypes of cells to platinum(II) complexes.

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Section: Cisplatin-membrane Interactionsmentioning

confidence: 99%

Cisplatin-Membrane Interactions and Their Influence on Platinum Complexes Activity and Toxicity

et al. 2019

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“…The biomembrane is postulated as the initial target when platinum (II) complexes attack cells. 33 Meanwhile platinum nanoparticles are a superoxide dismutase/catalase mimetic, 7 and convert hydrogen molecules to active hydrogen (atomic hydrogen). 10 In accordance with these studies, Pt/PVP-colloid or HW increased erythrocyte transformability and fluidity even in the presence of AAPH (Figs.…”

Section: Blood Fluidity and Erythrocyte Transforming Property Assessementioning

confidence: 99%

Colloidal Platinum in Hydrogen-Rich Water Exhibits Radical-Scavenging Activity and Improves Blood Fluidity

Kato

Hokama²,

Okayasu³

et al. 2012

j. nanosci. nanotech.

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The 'colloidal platinum' stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). And we evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and improvement of blood fluidity under 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. When applied with the 0.25-0.5 ppm Pt/PVP-colloid in RW or HW, the level of DPPH radicals decreased to 77.5-59.6% or 16.1-5.6%, in contrast to the level as high as 81.3% for HW alone, respectively, as measured by an electron spin resonance method. The horse blood, which was subjected to AAPH-induced oxidative stress, was incubated for 24 hr with RW or HW, and thereafter required 13.7 sec (100%) or 5.7 sec (42.3%) for passing through the micro-channels in a rheology equipment. When treated with 0.5-1.0 ppm Pt/PVP-colloid in RW or HW, the blood passage time in the micro-channels decreased dose-dependently to 9.7-7.3 sec (71.6-53.8%) or 4.3-1.3 sec (32.8-10.3%), and the rate of micro-channels clogged with erythrocyte aggregates decreased to 23.8-21.0% or 15.8-9.8%, respectively, from 42.8% for no addition of Pt/PVP. By scanning electron microscopy, AAPH-treated erythrocytes lost intact surface morphology on the membrane together with protrusions and without hollows, being indicative of impaired transforming ability, and the rate of erythrocyte agglutination was increased to 46.2%. When treated the horse blood with HW alone significantly decreased the rate of erythrocyte agglutination to 29.6%, whereas 1.0 ppm Pt/PVP-colloid in RW or HW decreased it to 24.1% or 21.1%, respectively. Thus, DPPH-radical-scavenging and erythrocyte-protecting effects of Pt/PVP-colloid in HW were superior to those of Pt/PVP-colloid in RW or Pt/PVP-free HW. The results could be mainly attributed to the enhanced antioxidant activity of Pt/PVP in HW, which may be due to captured-hydrogen on platinum.

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“…In the latter case, the cationic species may not be favored for interactions at this interface due to the hydrophobic characteristics created by height mismatch between lipids. Previous studies have reported that platinum complexes, including cisplatin and AqCis, were able to increase the permeability of erythrocyte membranes 38 and planar model membranes of hen's egg yolk (mostly composed of phospholipids of PC and PE), where the latter was attributed to the formation of small holes. 39 Our results suggest that indeed the permeability of the membrane is increased without major alterations of the lipid phase properties.…”

Section: Effects Of Cisplatin and Aqcis On Fluid And Gel Phasementioning

confidence: 99%

Effect of Cisplatin and Its Cationic Analogues in the Phase Behavior and Permeability of Model Lipid Bilayers

et al. 2023

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Increasing evidence suggests a critical role of lipids in both the mechanisms of toxicity and resistance of cells to platinum(II) complexes. In particular, cisplatin and other analogues were reported to interact with lipids and transiently promote lipid phase changes both in the bulk membranes and in specific membrane domains. However, these processes are complex and not fully understood. In this work, cisplatin and its cationic species formed at pH 7.4 in low chloride concentrations were tested for their ability to induce phase changes in model membranes with different lipid compositions. Fluorescent probes that partition to different lipid phases were used to report on the fluidity of the membrane, and a leakage assay was performed to evaluate the effect of cisplatin in the permeability of these vesicles. The results showed that platinum(II) complex effects on membrane fluidity depend on membrane lipid composition and properties, promoting a stronger decrease in the fluidity of membranes containing gel phase. Moreover, at high concentration, these complexes were prone to alter the permeability of lipid membranes without inducing their collapse or aggregation.

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Effects of Cisplatin and its Analogues on the Permeabilityof Human Erythrocyte Membrane

Cited by 9 publications

References 0 publications

Cisplatin-Membrane Interactions and Their Influence on Platinum Complexes Activity and Toxicity

Cisplatin-Membrane Interactions and Their Influence on Platinum Complexes Activity and Toxicity

Colloidal Platinum in Hydrogen-Rich Water Exhibits Radical-Scavenging Activity and Improves Blood Fluidity

Effect of Cisplatin and Its Cationic Analogues in the Phase Behavior and Permeability of Model Lipid Bilayers

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