Effects of chronic passive smoking on the regeneration of rat femoral defects filled with hydroxyapatite and stimulated by laser therapy

Franco, G.R.; Laraia, Isabela Ortiz; Maciel, Ana Alice Wolf; Miguel, N.M.; Santos, Geovane Ribeiro dos; Fabrega-Carvalho, C.A.; Pinto, Clóvis Antônio Lopes; Pettian, Mariane Silva; Cunha, Marcelo Rodrigues da

doi:10.1016/j.injury.2012.12.022

Cited by 17 publications

(6 citation statements)

References 36 publications

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“…The percent volume of newly formed bone in the recipient area was calculated in all groups at 2 and 6 weeks after surgery using a 100-point quadrilateral grid system coupled to the eyepiece of a light microscope. Newly formed bone was quantified according to the principle of Delesse using the formula Vv = Pp/Pt (%), where Vv is the volume density or relative volume, Pp is the number of points (line intersection) on newly formed bone, and Pt is the total number of points of the system (Lacerda, 1999;Franco et al, 2013). For each animal, 12 sections per specimen of the entire bone injury were measured.…”

Section: Histometric Analysismentioning

confidence: 99%

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

et al. 2015

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Section: Histometric Analysismentioning

confidence: 99%

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

et al. 2015

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“…In vivo murine models, on the other hand, have been reported to replicate the damage in bone integrity and the compromised bone regeneration outcome in tobacco consumers as well as in people exposed to secondhand smoke 24 – 26 . These models have also been used to study the effect of exposure to cigarette total particulate matter (TPM) in skin wound healing 27 , and to address the damage induced by cigarette smoke on the olfactory epithelium, the impairment of nasal mucosa healing and the recovery of olfactory receptor neurons 28 , 29 .…”

Section: Introductionmentioning

confidence: 99%

A Novel In Vivo Model to Study Impaired Tissue Regeneration Mediated by Cigarette Smoke

Alvarez

Chávez

Miranda

et al. 2018

Sci Rep

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Cigarette smoke is associated with several pathologies including chronic respiratory diseases and cancer. In addition, exposure to cigarette smoke is correlated with impaired wound healing, where a significant decrease in the regenerative capacity of smokers is well documented and broadly considered a negative risk factor after trauma or surgery. So far, some in vitro and in vivo models have been described to study how exposure to cigarette smoke diminishes the regenerative potential in different organisms. However, although useful, many of these models are difficult and expensive to implement and do not allow high-throughput screening approaches. In order to establish a reliable and accessible model, we have evaluated the effects of cigarette smoke extract (CSE) on zebrafish development and regeneration. In this work, zebrafish embryos and larvae were exposed to low doses of aqueous CSE showing severe developmental abnormalities in a dose-dependent manner. Furthermore, when adult zebrafish were subjected to caudal fin amputation, we observed a significant decrease in the regenerative capacity of animals exposed to CSE. The effect was exacerbated in male and aged fish compared to female or young organisms. The establishment of a zebrafish model to assess the consequences of cigarette smoke and its effects on animal physiology could provide a new tool to study the underlying mechanisms involved in impaired tissue regeneration, and aid the development of novel approaches to treat complications associated with cigarette smoke toxicity.

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“…In addition, this biomaterial is atoxic and has a controllable microstructure in terms of pore size 6 . In view of its osteogenic properties, hydroxyapatite is widely used in bone reconstruction surgeries [7][8][9] . Another material used mainly for the regeneration of soft tissues is fibrin sealant, which serves as a scaffold for cell adhesion and growth, in addition to presenting hemostatic properties.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

et al. 2015

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Biomaterials are used as a promising alternative to bone grafts, including bioceramics whose composition resembles that of bone and fibrin sealants due to their hemostatic properties. The objective was to evaluate the repair of cranial defects in 40 rats, grafted with hydroxyapatite and a new fibrin sealant derived from snake venom. The animals were divided into four groups: C (control, no graft); Ha (hydroxyapatite); FS (fibrin sealant), and HaFS (hydroxyapatite and fibrin sealant). The animals were euthanized 2 and 6 weeks after surgery and wound area were submitted to analysis. After 2 weeks, immature bone was formed from the borders of the defect and in groups Ha and HaFS, few hydroxyapatite particles were surrounded by new bone. After 6 weeks, the new bone was mature and surrounded several hydroxyapatite particles, without connective tissue interposition and the volume of new bone was higher in HaFS group. The hydroxyapatite in combination with the new fibrin sealant accelerates bone repair.

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Effects of chronic passive smoking on the regeneration of rat femoral defects filled with hydroxyapatite and stimulated by laser therapy

Cited by 17 publications

References 36 publications

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

A Novel In Vivo Model to Study Impaired Tissue Regeneration Mediated by Cigarette Smoke

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

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