Effects of Chitosan on Properties of Novel Human-like Collagen/Chitosan Hybrid Vascular Scaffold

Abstract

Novel human-like collagen (HLC)/chitosan hybrid scaffolds were fabricated at blend ratios of 0%, 0.02%, 0.2% by crosslinking and freeze-drying process. The properties of the scaffolds were investigated, including morphology, mechanical strength, degradability, and cell biocompatibility. When the blend ratio was 0.02%, the morphology of the scaffolds was highly homogeneous with interconnected porous structure 46 AE 9 mm in size (SEM). The X-ray photoelectron spectroscopy analysis indicated intermolecular crosslinks between HLC and chitosan. The strain and stress of the scaffolds were 37.9 AE 3.3% and 309.7 AE 19.7 KPa, respectively. Human venous fibroblasts were expanded and seeded into the scaffolds in the density of 1 Â 10 5 cells/cm 3 under static conditions. The cell morphology and proliferation were investigated using SEM, H&E, and MTT assay, which showed that the optimal content of the chitosan was signifcantly enhanced the cells adhesion, proliferation, and viability, compared to pure HLC, pure chitosan, and 0.2% chitosan/HLC scaffolds. These hybrid scaffolds appear to have favorable characteristics for vascular tissue engineering application.

Human-like collagen/nano-hydroxyapatite scaffolds for the culture of chondrocytes

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Encapsulation of probiotic Bifidobacterium longum BIOMA 5920 with alginate–human-like collagen and evaluation of survival in simulated gastrointestinal conditions

“…Currently, human-like collagen is used as a novel hemostatic material [17], a novel scaffolding biomaterial for artificial bones [18,19], skin tissues [20], blood vessel tissues [21,22] and functional foods. HLC is a water-soluble and fibrous protein, which does not require denatured treatment compared with some globular proteins and could easily incorporate into the matrix of alginate due to an interaction of some groups of HLC with carboxyl groups of alginate.…”

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