Chitosan Membrane with Surface-bonded Growth Factor in Guided Tissue Regeneration Applications

Chang, Chih-Hao; Tsao, Ching-Ting; Chang, Ken-Yu; Wang, Jaw‐Lin; Young, Tai‐Horng; Han, J. L.; Hsieh, K. H.

doi:10.1177/0883911510372284

Cited by 16 publications

(9 citation statements)

References 31 publications

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“…Subsequently, the catalyst solution was added dropwise into the mixture of chitosan and mPEG-acid. The solution was stirred for 4 hr at room temperature allowing for amide linkage formation between chitosan and mPEG-acid [23,29,30]. 0.5 M NaOH was added dropwise until a pH value of 7 was reached.…”

Section: Methodsmentioning

confidence: 99%

PEG-Chitosan Hydrogel with Tunable Stiffness for Study of Drug Response of Breast Cancer Cells

et al. 2016

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Mechanical properties of the extracellular matrix have a profound effect on the behavior of anchorage-dependent cells. However, the mechanisms that define the effects of matrix stiffness on cell behavior remains unclear. Therefore, the development and fabrication of synthetic matrices with well-defined stiffness is invaluable for studying the interactions of cells with their biophysical microenvironment in vitro. We demonstrate a methoxypolyethylene glycol (mPEG)-modified chitosan hydrogel network where hydrogel stiffness can be easily modulated under physiological conditions by adjusting the degree of mPEG grafting onto chitosan (PEGylation). We show that the storage modulus of the hydrogel increases as PEGylation decreases and the gels exhibit instant self-recovery after deformation. Breast cancer cells cultured on the stiffest hydrogels adopt a more malignant phenotype with increased resistance to doxorubicin as compared with cells cultured on tissue culture polystyrene or Matrigel. This work demonstrates the utility of mPEG-modified chitosan hydrogel, with tunable mechanical properties, as an improved replacement of conventional culture system for in vitro characterization of breast cancer cell phenotype and evaluation of cancer therapies.

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Section: Methodsmentioning

confidence: 99%

PEG-Chitosan Hydrogel with Tunable Stiffness for Study of Drug Response of Breast Cancer Cells

et al. 2016

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“…These materials provide the benefit of avoiding unwanted immunological responses, and they eliminate the risk of acquiring infectious diseases (AIDS and hepatitis) from graft tissue and body fluid [ 1 ]. Therefore, bone scaffold, which is biomimetic in both structure and chemical factor coating, is usually used for bone surgery to repair defects [ 2 , 3 ]. The selection of materials for bone scaffolds must incorporate the consideration of issues such as mechanical properties and bonding strength at the scaffold-bone interface.…”

Section: Introductionmentioning

confidence: 99%

3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity

et al. 2015

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Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

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“…Taiwan researchers produced a CHI surface-bonded recombinant human bone morphogenetic protein 2 via amide bond formation between the components. The surface-bonded protein did not denature but expressed sustained biological activity, such as osteoblast cell adhesion, proliferation, and differentiation, so making the material useful for bone tissue regeneration [50]. Korean researchers prepared porous, biodegradable and biocompatible scaffolds, using CHI, CHI with natural hydroxyapatite derived from Thunnus Obesus bone, and CHI grafted with functionalized multiwalled carbon nanotube, via freeze-drying method, and characterized them physiochemically as bone graft substitutes.…”

Section: Bone and Cartilage Regenerationmentioning

confidence: 99%

Chitosan-Based Macromolecular Biomaterials for the Regeneration of Chondroskeletal and Nerve Tissue

Guerra

Barbani

Gagliardi

et al. 2011

International Journal of Carbohydrate Chemistry

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The use of materials, containing the biocompatible and bioresorbable biopolymer poly(1→4)-2-amino-2-deoxy-β-D-glucan, containing some N-acetyl-glucosamine units (chitosan, CHI) and/or its derivatives, to fabricate devices for the regeneration of bone, cartilage and nerve tissue, was reviewed. The CHI-containing devices, to be used for bone and cartilage regeneration and healing, were tested mainly for in vitro cell adhesion and proliferation and for insertion into animals; only the use of CHI in dental surgery has reached the clinical application. Regarding the nerve tissue, only a surgical repair of a 35 mm-long nerve defect in the median nerve of the right arm at elbow level with an artificial nerve graft, comprising an outer microporous conduit of CHI and internal oriented filaments of poly(glycolic acid), was reported. As a consequence, although many positive results have been obtained, much work must still be made, especially for the passage from the experimentation of the CHI-based devices, in vitro and in animals, to their clinical application.

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Chitosan Membrane with Surface-bonded Growth Factor in Guided Tissue Regeneration Applications

Cited by 16 publications

References 31 publications

PEG-Chitosan Hydrogel with Tunable Stiffness for Study of Drug Response of Breast Cancer Cells

PEG-Chitosan Hydrogel with Tunable Stiffness for Study of Drug Response of Breast Cancer Cells

3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity

Chitosan-Based Macromolecular Biomaterials for the Regeneration of Chondroskeletal and Nerve Tissue

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