Effects of chilling temperature on photosynthetic rates, photosynthetic enzyme activities and metabolite levels in leaves of three sugarcane species

Du, YC; Nose, Akihiro; Wasano, Kikuo

doi:10.1046/j.1365-3040.1999.00415.x

Cited by 82 publications

(56 citation statements)

References 28 publications

(37 reference statements)

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“…This is consistent with the observation that there are few amino acid changes that distinguish C 4 PPDK sequences among M. 3 giganteus, maize, and cold-intolerant sugar cane (Naidu et al, 2003). Previous analyses of sugar cane lines with different cold sensitivity (Du et al, 1999a) and Echinochloa crus-galli ecotypes from contrasting climates (Simon, 1996) also concluded that cold tolerance in those species is not due to the intrinsic properties of PPDK. The difference in crude extracts is therefore the result of either posttranslational modification or the presence of activators or inhibitors that affect the activity of the enzyme in leaf extracts.…”

Section: Discussionsupporting

confidence: 87%

“…The subunits dissociate between 10°C and 15°C, causing an increase in the energy of activation (E a ) and a loss of catalytic rate with further decline in temperature (Shirahashi et al, 1978;Ohta et al, 1996;Du et al, 1999b). In addition, the maximum extractable PPDK activity correlated well with cold tolerance in three Saccharum species (Du et al, 1999a) and across maize cultivars (Sugiyama and Boku, 1976). In contrast to the effects of temperature on the PPDK enzyme, there appears to be no correlation between thermal properties of other C 4 photosynthetic enzymes and differences in cold sensitivity of plant ecotypes (Du et al, 1999b;Hamel and Simon, 2000).…”

mentioning

confidence: 95%

“…M. 3 giganteus avoids this damage both by maintaining a high rate of CO 2 uptake, to increase utilization of absorbed light (Beale et al, 1996), and by increased nonphotochemical quenching, which correlates with a large increase in xanthophyll content and its de-epoxidation (Farage et al, 2006). Chilling-induced decreases in the leaf photosynthetic rate of CO 2 uptake of C 4 species have been correlated with decreases in: carboxylation efficiency via PEPc (Kingston-Smith et al, 1997;Chinthapalli et al, 2003), capacity for PEP regeneration via PPDK (Du et al, 1999a), Rubisco activity (Kingston- Smith et al, 1997;Du et al, 1999a;Pittermann and Sage, 2000;Pittermann and Sage, 2001;Chinthapalli et al, 2003), or by a combination of these. Analyses of flux control coefficients in transgenic plants of the C 4 dicot Flaveria bidentis suggest that Rubisco and PPDK share control and limit light-saturated C 4 photosynthesis (Furbank et al, 1997).…”

mentioning

confidence: 99%

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Cool C4 Photosynthesis: Pyruvate Pi Dikinase Expression and Activity Corresponds to the Exceptional Cold Tolerance of Carbon Assimilation inMiscanthus×giganteus

et al. 2008

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The bioenergy feedstock grass Miscanthus 3 giganteus is exceptional among C 4 species for its high productivity in cold climates. It can maintain photosynthetically active leaves at temperatures 6°C below the minimum for maize (Zea mays), which allows it a longer growing season in cool climates. Understanding the basis for this difference between these two closely related plants may be critical in adapting maize to colder weather. When M. 3 giganteus and maize grown at 25°C were transferred to 14°C, light-saturated CO 2 assimilation and quantum yield of photosystem II declined by 30% and 40%, respectively, in the first 48 h in these two species. The decline continued in maize but arrested and then recovered partially in M. 3 giganteus. Within 24 h of the temperature transition, the pyruvate phosphate dikinase (PPDK) protein content per leaf area transiently declined in M. 3 giganteus but then steadily increased, such that after 7 d the enzyme content was significantly higher than in leaves growing in 25°C. By contrast it declined throughout the chilling period in maize leaves. Rubisco levels remained constant in M. 3 giganteus but declined in maize. Consistent with increased PPDK protein content, the extractable PPDK activity per unit leaf area (V max,ppdk ) in cold-grown M. 3 giganteus leaves was higher than in warm-grown leaves, while V max,ppdk was lower in coldgrown than in warm-grown maize. The rate of light activation of PPDK was also slower in cold-grown maize than M. 3 giganteus. The energy of activation (E a ) of extracted PPDK was lower in cold-grown than warm-grown M. 3 giganteus but not in maize. The specific activities and E a of purified recombinant PPDK from M. 3 giganteus and maize cloned into Escherichia coli were similar. The increase in PPDK protein in the M. 3 giganteus leaves corresponded to an increase in PPDK mRNA level. These results indicate that of the two enzymes known to limit C 4 photosynthesis, increase of PPDK, not Rubisco content, corresponds to the recovery and maintenance of photosynthetic capacity. Functionally, increased enzyme concentration is shown to increase stability of M. 3 giganteus PPDK at low temperature. The results suggest that increases in either PPDK RNA transcription and/or the stability of this RNA are important for the increase in PPDK protein content and activity in M. 3 giganteus under chilling conditions relative to maize.

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Section: Discussionsupporting

confidence: 87%

mentioning

confidence: 95%

mentioning

confidence: 99%

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Cool C4 Photosynthesis: Pyruvate Pi Dikinase Expression and Activity Corresponds to the Exceptional Cold Tolerance of Carbon Assimilation inMiscanthus×giganteus

et al. 2008

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“…The cold sensitivity of PPDK in C 4 plants is well established and the mechanism of cold inactivation of PPDK is studied in detail [15,[17][18]. In contrast, the reports on cold sensitivity of PEPC have been quite conflicting.…”

Section: Introductionmentioning

confidence: 99%

Temperature Modulation of the Activity and Malate Inhibition of the Phosphoenolpyruvate Carboxylase from Leaves of Alternanthera pungens, Compared to that of Lycopersicom esculentum

Chinthapalli

Chitra²,

Raghavendra³

2014

AJBIO

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Abstract:Temperature caused marked modulation of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in leaf discs of Alternanthera pungens (C 4 plant) as well as Lycopersicon esculentum (C 3 species). The optimal incubation temperature for PEPC activity in A. pungens was 45 °C compared to 30 °C in L. esculentum. A. pungens lost nearly 61% of PEPC activity on exposure to a low temperature of 15 °C, compared to only about a 33% loss in the case of L. esculentum. The C 4 enzyme was less sensitive to supra-optimal temperature and more sensitive to sub-optimal temperature than that of the C 3 species. Further as the temperature was raised from 15 °C to 50 °C, there was a sharp decrease in malate sensitivity of PEPC. The extent of such a decrease in C 4 plants was more than that in C 3 species. Arrhenius plots that were constructed by plotting the activity of PEPC against the reciprocal of temperature in the absence or presence of malate exhibited abrupt changes or "break-points" at only one point of 17 o C in A. pungens while at two points corresponding 17 o C and 27 o C in case of L. esculentum. The activation energy of PEPC from A. pungens was less compared to that of L. esculentum in the temperature range of 10 to 27 o C. However, the activation energy of PEPC from A. pungens was less than that of L. esculentum above the temperature of 27 o C. The activation energy increased by 2 to 4 fold at temperatures below 17 o C, in case of both A. pungens and L. esculentum. Thus, our results show the activity and malate sensitivity of PEPC can be influenced in relation to high temperature tolerance of C 4 plants, which can be physiologically significant.

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“…is an important tropical grass crop that suffers from abiotic stresses caused by low temperatures and drought (Robertson et al, 1999;Tai and Lentini, 2002;Silva et al, 2008). These stresses can reduce sugarcane photosynthetic rates considerably, and a decrease by more than 50% was observed after exposure to temperatures of 10ºC for 3 days (Du et al, 1999). This can lead to alterations in sugar quality and reduction in biomass.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

Ditt

Gentile

Tavares

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a coldsensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC). With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.

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Effects of chilling temperature on photosynthetic rates, photosynthetic enzyme activities and metabolite levels in leaves of three sugarcane species

Cited by 82 publications

References 28 publications

Cool C4 Photosynthesis: Pyruvate Pi Dikinase Expression and Activity Corresponds to the Exceptional Cold Tolerance of Carbon Assimilation inMiscanthus×giganteus

Cool C4 Photosynthesis: Pyruvate Pi Dikinase Expression and Activity Corresponds to the Exceptional Cold Tolerance of Carbon Assimilation inMiscanthus×giganteus

Temperature Modulation of the Activity and Malate Inhibition of the Phosphoenolpyruvate Carboxylase from Leaves of Alternanthera pungens, Compared to that of Lycopersicom esculentum

Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

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