Comparative Study on the Effect of Organic and Inorganic Fertilizers on Agronomic Performance of Faba Bean (Vicia faba L.) and Pea (Pisum sativum L.). Agriculture, Forestry and Fisheries. Vol. 4, No. 6, 2015, pp. 263-268. doi: 10.11648/j.aff.20150406.15 Abstract: The study was conducted to observe the comparative effect of organic fertilizer (cow dung) and inorganic fertilizers like urea and potassium chloride on the growth, biomass and biochemical parameters of two legumes of pea (Pisum sativum) and faba bean (Vicia faba). Experiments were done using two plant species of legume family. Organic fertilizer like cow dung (15t/ha) and inorganic fertilizer was applied at rate of urea (120kg/ha) and potassium chloride (125kg/ha). The application of cow dung at 15t/ha showed significant growth over the inorganic fertilizer urea and potassium chloride in terms of germination percentage, fresh weight and dry weight, plant height, shoot length, and root length as well as number of leaves in both the legume plants. Similarly, biochemical parameters have also shown significant differences from organic fertilizer over the inorganic fertilizers and control. Thus our study provides the evidence for using organic fertilizer like cow dung by farmers to have better yield to produce quality grains as cow dung is easy available, environmentally safe and cost effective in pea and faba bean plants.
Shoot meristems used for the study were exercised from the in vitro regenerated shoots cultured on MS medium supplemented with 0.5 mg/L of BAP for multiplication. The sensitivity of the in vitro regenerated was studied using shoot meristems of 0.5 cm. Shoot meristems were cultured on medium containing 10 -100 mg/l kanamycin to determine the concentration that was lethal for multiple shoot induction and root induction.
Abstract:Temperature caused marked modulation of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in leaf discs of Alternanthera pungens (C 4 plant) as well as Lycopersicon esculentum (C 3 species). The optimal incubation temperature for PEPC activity in A. pungens was 45 °C compared to 30 °C in L. esculentum. A. pungens lost nearly 61% of PEPC activity on exposure to a low temperature of 15 °C, compared to only about a 33% loss in the case of L. esculentum. The C 4 enzyme was less sensitive to supra-optimal temperature and more sensitive to sub-optimal temperature than that of the C 3 species. Further as the temperature was raised from 15 °C to 50 °C, there was a sharp decrease in malate sensitivity of PEPC. The extent of such a decrease in C 4 plants was more than that in C 3 species. Arrhenius plots that were constructed by plotting the activity of PEPC against the reciprocal of temperature in the absence or presence of malate exhibited abrupt changes or "break-points" at only one point of 17 o C in A. pungens while at two points corresponding 17 o C and 27 o C in case of L. esculentum. The activation energy of PEPC from A. pungens was less compared to that of L. esculentum in the temperature range of 10 to 27 o C. However, the activation energy of PEPC from A. pungens was less than that of L. esculentum above the temperature of 27 o C. The activation energy increased by 2 to 4 fold at temperatures below 17 o C, in case of both A. pungens and L. esculentum. Thus, our results show the activity and malate sensitivity of PEPC can be influenced in relation to high temperature tolerance of C 4 plants, which can be physiologically significant.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.