Effects of chemical contaminants on the health of Mytilus edulis from Puget Sound, Washington. II. Cytochemical detection of subcellular changes in digestive cells

Krishnakumar, P. K.; Casillas, Edmundo; Varanasi, Usha

doi:10.1007/bf00347129

Cited by 44 publications

(21 citation statements)

References 34 publications

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“…For example, when three biomarkers were considered together for the first eight months of the study, two or more exhibited a similar trend between the two sites to results documented previously in Krishnakumar et al (1994Krishnakumar et al ( , 1995. When all four biomarkers were available for the final 6 months, the same trend was exhibited in these parameters 75% of the time.…”

Section: Cytochemical Parameterssupporting

confidence: 84%

“…These cytochemical responses were measured previously in mussels from various areas of Puget Sound, Washington (Krishnakumar et al 1995). High levels of chemical pollutants, including polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), are found in sediments and biota at several of these locations (NOAA 1989;Stein et al 1992).…”

mentioning

confidence: 76%

“…A section of the digestive gland was removed from 10 randomly selected mussels per site per month (Krishnakumar et al 1994(Krishnakumar et al , 1995. Five tissue sections were placed in straight rows across the center of each cryomold (Tissue-Tek, Miles, Elkhart, IN).…”

Section: Cytochemical Parametersmentioning

confidence: 99%

“…The digestive gland (or digestive tubules) is the primary location of digestion and absorption of nutrients in mussels and is typically a site of high contaminant accumulation. Accumulation of contaminants can induce changes in a wide range of physiological and cytochemical parameters in the digestive gland (Bayne et al 1988;Bayne 1989;Gray 1992;Krishnakumar et al 1995). These laboratory and field studies show not only that exposure to chemical contaminants have biological consequences but moreover that these physiological responses may serve as indicators of contaminant exposure.…”

mentioning

confidence: 99%

“…Interpreting and attributing differences in organism responses to contaminants and to seasonal variation can be problematic. Previous field studies with mussels (Krishnakumar et al 1994(Krishnakumar et al , 1995(Krishnakumar et al , 1997 could not address the potential interactive effect of these exogenous factors due to limited temporal sampling (mussels were only sampled at one time period).…”

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confidence: 99%

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Assessment of Seasonal Variability of Cytochemical Responses to Contaminant Exposure in the Blue Mussel Mytilus edulis (Complex)

Kagley

Snider

Krishnakumar

et al. 2003

Archives of Environmental Contamination and Toxicology

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Abstract.A selected suite of cytochemical parameters in Mytilus edulis are altered in response to field and laboratory exposure to chemical contaminants. These biomarkers include lysosomal stability, nicotinamide adenine dinucleotide phosphate (NADPH)-ferrihemoprotein reductase activity, liposfuscin deposition, and accumulation of lysosomal and cytoplasmic unsaturated neutral lipid. Normal variations in physiological processes (influenced by exogenous seasonal changes in temperature, salinity, food availability, etc.) may alter the sensitivity of these biomarkers to contaminant exposure. To address this issue, M. edulis (complex) were sampled monthly from a reference nonurban site (Coupeville, Penn Cove) and a polluted urban site (Seacrest, Elliott Bay) in Puget Sound, WA, for a period of 15 months. Physiological measurements including total length, total weight, somatic and mantle weights (an indication of gonadal development and reproductive status), condition index, and the presence or absence of hemic neoplasia (HN, or leukemia) were recorded. Significant differences in lysosomal stability, lysosomal and cytoplasmic unsaturated neutral lipids, lipofuscin deposition, and NADPH-ferrihemoprotein reductase activity in cells of the digestive gland or digestive tubules were generally found in mussels taken throughout the year from Seacrest compared to mussels sampled from Coupeville, consistent with exposure to chemical contaminants. No seasonally influenced suppression of the entire suite of parameters as measures of contaminant exposure was evident. Therefore these biomarkers can be used to evaluate contaminant exposure in mussels throughout the entire year.

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Section: Cytochemical Parameterssupporting

confidence: 84%

mentioning

confidence: 76%

Section: Cytochemical Parametersmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Assessment of Seasonal Variability of Cytochemical Responses to Contaminant Exposure in the Blue Mussel Mytilus edulis (Complex)

Kagley

Snider

Krishnakumar

et al. 2003

Archives of Environmental Contamination and Toxicology

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Effects of Pollution on Fish

Lawrence¹,

Hemingway²

2003

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For further information on Blackwell Publishing, visit our website: www.blackwellpublishing.com ContentsList of Contributors xiii Preface xv Acknowledgements xviii Contents xi 7.4 The evolution of tolerance 7.4.1 Intrapopulation diversity 7.4.2 Interpopulation differentiation 7.4.3 The speed of adaptation 7.4.4 The costs of adaptation 7.4.5 The identification of tolerance genes 7.5 References 8 From Population Ecology to Socio-Economic and Human Health Issues 8.1 Introduction 8.1.1 Aims and objectives 8.1.2 The bio-socio-economic model 8.2 The fish sector of the European Union 8.2.1 Introduction 8.2.2 The Common Fisheries Policy (CFP) 8.2.3 The crisis in EU fisheries: interdependence or independence in relation to xenobiotic influences? 8.3 The quality of individual fish (intrinsic and extrinsic characteristics), scarcity and its effects on consumer health and behaviour 8.3.1 Intrinsic quality in fish 8.3.2 Extrinsic quality in fish 8.3.3 The fish trade and quality 8.4 Xenobiotic influences on fish quality 8.4.1 Ciguatoxin and red tides 8.4.2 Organochlorine pesticides 8.4.3 Heavy metals 8.4.4 The effects of hydrocarbons 8.5 Case studies 8.5.1 Oil spills 8.5.1.1 The Exxon Valdez oil spill 8.5.1.2 The Braer oil spill 8.5.1.3 The Sea Empress oil spill 8.5.2 Claims and compensations 8.6 Conclusions 8.7 References 9 The Role of Modelling in Fish and Fishery Ecotoxicology 9.1 Introduction 9.2 Summary of the effects of pollution on fish 9.2.1 Cellular and molecular responses 9.2.2 Damage to DNA 9.2.3 Physiological responses xii Contents 9.2.4 Immune system responses 9.2.5 Reproductive system responses 9.2.6 Population responses 9.2.7 Population genetic responses 9.2.8 Socio-economic response 9.3 The role of modelling of pollution impacts on fish and fisheries 9.3.1 Individual-based models 9.3.2 Population-based models 9.3.3 Ecosystem-based models 9.3.4 New bioeconomic models incorporating sublethal pollution impacts 9.3.5 The validity of modelling 9.4 Gaps in current understanding 9.4.1 Molecular and cellular response and genotoxicity 9.4.2 Molecular and cellular response and physiological processes 9.4.3 Molecular and cellular response and immune effects 9.4.4 Molecular and cellular response and reproduction 9.4.5 Population responses 9.4.6 Population genetic responses 9.4.7 Socio-economic impact 9.

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