Effects of cerium oxide on liver tissue in liver ischemia‑reperfusion injury in rats undergoing sevoflurane anesthesia

Göbüt, Hüseyin; Erel, Selin; Ozdemir, Cagri; Mortaş, Tülay; Arslan, Mustafa; Küçük, Ayşegül; Kasapbası, Esat; Kavutçu, Mustafa

doi:10.3892/etm.2023.11863

Cited by 3 publications

(4 citation statements)

References 45 publications

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“…39 TBARS, AES, GST, and CAT levels serve as indicators of oxidative stress. 28 In the present study, the hepatic and kidney tissues of rats subjected to dexmedetomidine before pancreatic IR injury exhibited significant reductions in their TBARS, AES, and CAT levels compared to those in the IR group. However, there were no differences between the GST levels of the groups.…”

Section: Discussionsupporting

confidence: 46%

“…The semiquantitative evaluation technique used by Abdel-Wahhab et al 27 for histological testing was applied to interpret the structural changes in the hepatic tissues of the control and treatment groups, with a negative point (0) representing no structural changes, one positive point (1, +) indicating mild changes, two positive points (2, ++) representing medium structural changes, and three positive points (3, +++) indicating severe structural changes. 28…”

Section: Livermentioning

confidence: 99%

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Dexmedetomidine’s Effects on the Livers and Kidneys of Rats with Pancreatic Ischemia-Reperfusion Injury

Bostancı,

Erel,

Küçük

et al. 2024

DDDT

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Pancreatic surgeries inherently cause ischemia-reperfusion (IR) injury, affecting not only the pancreas but also distant organs. This study was conducted to explore the potential use of dexmedetomidine, a sedative with antiapoptotic, anti-inflammatory, and antioxidant properties, in mitigating the impacts of pancreatic IR on kidney and liver tissues. Methods: A total of 24 rats were randomly divided into four groups: control (C), dexmedetomidine (D), ischemia reperfusion (IR), and dexmedetomidine ischemia reperfusion (D-IR). Pancreatic ischemia was induced in the IR and D-IR groups. Dexmedetomidine was administered intraperitoneally to the D and D-IR groups. Liver and kidney tissue samples were subjected to microscopic examinations after hematoxylin and eosin staining. The levels of thiobarbituric acid reactive substances (TBARS), aryllesterase (AES), catalase (CAT), and glutathione S-transferase (GST) enzyme activity were assessed in liver and kidney tissues. The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine were measured. Results: A comparison of the groups revealed that the IR group exhibited significantly elevated TBARS (p < 0.0001), AES (p = 0.004), and CAT enzyme activity (p < 0.0001) levels in the liver and kidney compared to groups C and D. Group D-IR demonstrated notably reduced histopathological damage (p < 0.05) and low TBARS (p < 0.0001), AES (p = 0.004), and CAT enzyme activity (p < 0.0001) in the liver and kidney as well as low AST and ALT activity levels (p < 0.0001) in the serum compared to the IR group. Conclusion:The preemptive administration of dexmedetomidine before pancreatic IR provides significant protection to kidney and liver tissues, as evidenced by the histopathological and biochemical parameters in this study. The findings underscored the potential therapeutic role of dexmedetomidine in mitigating the multiorgan damage associated with pancreatic surgeries.

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Section: Discussionsupporting

confidence: 46%

Section: Livermentioning

confidence: 99%

Dexmedetomidine’s Effects on the Livers and Kidneys of Rats with Pancreatic Ischemia-Reperfusion Injury

Bostancı,

Erel,

Küçük

et al. 2024

DDDT

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“…As mentioned before, IR injury increases ROS and RNS and decreases antioxidant enzyme levels and expression, according to several studies [22]. Numerous enzymes perform as intracellular antioxidants to protect cells from IR-induced oxidative damage [23]. In this manuscript, we determined the CAT enzyme activity for cellular antioxidant defense functions.…”

Section: Discussionmentioning

confidence: 86%

The Effect of “Proanthocyanidin” on Ischemia–Reperfusion Injury in Skeletal Muscles of Rats

Özer,

Koçak,

Sezen

et al. 2024

Medicina

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Background and Objectives: Lower limb skeletal muscle ischemia–reperfusion (IR) injury is associated with increased morbidity and mortality, and it is common in several clinical situations such as aortic aneurysms repairment, peripheral arterial surgery, vascular injury repairment, and shock. Although it is generally accepted that oxidative stress mediators have a significant role in IR injury, its precise mechanism is still unknown. Anecdotally, it is sustained not only by structural and functional changes in the organ it affects but also by damage to distant organs. The purpose of this report is to illustrate the effect of proanthocyanidin on IR injury. Materials and Methods: In our study, 18 male Wistar albino rats were used. The subjects were divided into three groups containing six mice each (control, C; ischemia–reperfusion, IR; ischemia–reperfusion and proanthocyanidin; IR-PRO). Intraperitoneal proanthocyanidin was given to the IR and proanthocyanidin groups 30 min before laparotomy, and 1 h ischemia led to these two groups. After one hour, reperfusion started. Muscle atrophy–hypertrophy, muscle degeneration–congestion, fragmentation–hyalinization, muscle oval-central nucleus ratio, leukocyte cell infiltration, catalase enzyme activity, and TBARS were all examined in lower-limb muscle samples after one hour of reperfusion. Results: When skeletal muscle samples were evaluated histopathologically, it was discovered that muscle atrophy–hypertrophy, muscle degeneration–congestion, fragmentation–hyalinization, and leukocyte cell infiltration with oval-central nucleus standardization were significantly higher in the IR group than in the C and IR-P groups. Oval-central nucleus standardization was significantly higher in the IR and IR-PRO groups than in the control group. TBARS levels were significantly higher in the IR group than in the control and IR-PRO groups, while catalase enzyme activity was found to be significantly lower in the IR group than in the control and IR-PRO groups. Conclusions: As a consequence of our research, we discovered that proanthocyanidins administered before IR have a protective impact on skeletal muscle in rats. Further research in this area is required.

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“…Numerous enzymes perform as intracellular antioxidants to protect cells from IR-induced oxidative damage. [19] In this manuscript, we determined CAT enzyme activity for cellular antioxidant defense functions. In this regard, our study supported the literature by increasing the CAT enzyme in Group IR-PRO and Group C compared to Group IR.…”

Section: Discussionmentioning

confidence: 99%

The Effect of “Proanthocyanidin” on Ischemia-Reperfusion Injury in Skeletal Muscles of Rats

Özer,

Koçak,

Sezen

et al. 2024

Preprint

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BACKGROUND: Lower limb skeletal muscle ischemia-reperfusion (IR) injury associated with increased morbidity and mortality is common in several clinical situations such as aortic aneurysms repairment, peripheral arterial surgery, vascular injury repairment, and shock. Although it is generally accepted that oxidative stress mediators have a significant role in IR injury, its precise mechanism is still unknown. Anecdotally, it is sustained not only by structural and functional changes in the organ it affects but also by damage to distant organs. The purpose of this report is to illustrate the effect of proanthocyanidin on IR injury. METHODS: In our study, 18 male Wistar albino rats were used. The subjects were divided into three groups of six each. (Group Control-C, Group Ischemia-Reperfusion -IR, Group Ischemia-Reperfusion and Proanthocyanidin-IR-PRO). Intraperitoneal proanthocyanidin was given to the IR and proanthocyanidin groups 30 minutes before laparotomy and 1-hour ischemia led to these two groups. After one hour, reperfusion started. Muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, muscle oval-central nucleus ratio, leukocyte cell infiltration, catalase enzyme activity, and TBARS were all examined in lower-limb muscle samples after one hour of reperfusion. RESULTS: When skeletal muscle samples were evaluated histopathologically, it was discovered that muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, and leukocyte cell infiltration with oval-central nucleus standardization were significantly higher in the IR group than group control and group IR-proanthocyanidin. Besides, oval-central nucleus standardization was significantly higher in the IR and proanthocyanidin group than in the control group. TBARS levels were significantly higher in the IR group than in the control and IR-proanthocyanidin groups while, catalase enzyme activity was found to be significantly lower in the IR group than in the control and IR and proanthocyanidin groups. CONCLUSION: As a consequence of our research, we discovered that proanthocyanidins administered before IR have a protective impact on skeletal muscle in rats. Further research in this area is required.

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Effects of cerium oxide on liver tissue in liver ischemia‑reperfusion injury in rats undergoing sevoflurane anesthesia

Cited by 3 publications

References 45 publications

Dexmedetomidine’s Effects on the Livers and Kidneys of Rats with Pancreatic Ischemia-Reperfusion Injury

Dexmedetomidine’s Effects on the Livers and Kidneys of Rats with Pancreatic Ischemia-Reperfusion Injury

The Effect of “Proanthocyanidin” on Ischemia–Reperfusion Injury in Skeletal Muscles of Rats

The Effect of “Proanthocyanidin” on Ischemia-Reperfusion Injury in Skeletal Muscles of Rats

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