Effects of Carnosine and Related Compounds on the Stability and Morphology of Erythrocytes From Alcoholics

Prokopieva, V. D.; Bohan, Nikolay; Johnson, Peter; Abe, Hiroki; Boldyrev, A. A.

doi:10.1093/alcalc/35.1.44

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…Our results indicate that the effects of this compound (L-carnosine ) on erythrocytes cannot be explained simply by its antioxidant properties, but may be related to other effect of it, such as direct interaction with membrane components or modulation of erythrocytes enzyme activities, which are components of the membrane repair systems [11]. It can be also suggested that this dipeptide may be become integrated into the erythrocytes membrane.…”

Section: Discussionmentioning

confidence: 75%

Impaired erythrocytes deformability in H2O2-induced oxidative stress: Protective effect of L-carnosine

Aydoğan

Yapışlar²,

Artis³

et al. 2008

Clinical Hemorheology and Microcirculation

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Impaired red blood cell deformability is a hemorheological perturbation induced by many kinds of diseases. An increase in free radicals causes a reduction in erythrocyte flexibility and deformability. Carnosine is a dipeptide abundant in skeletal muscle and brain of humans. One of the main function of carnosine is its antioxidant and free-radical scavenger effect. In this study our aim is to investigate the protective effect of L-carnosine on RBCs in H 2 O 2 -induced oxidative stress in vitro conditions.Twenty male wistar albino rats, 10 were 3 months old, 10 were 12 months old used. The blood from each rat were divided into ten tubes and these blood samples divided into two groups. The first tube of the first group was the control and the rest 4 tubes were treated with different concentrations of L-carnosine. All tubes in the second group were incubated with H 2 O 2 additively. The deformability indexes of the erythrocytes were measured by a laser diffractometer (Myrenne Rheodyne SSD).L-carnosine has improved the RBC deformability significantly which is impaired by H 2 O 2 treatment (p < 0.05). Increase in deformability is more significant in young rat group when compared to old rat group.L-carnosine, as an antioxidant molecules, has a dose dependent positive effect on RBC deformability and has improved or protect the deformability of erythrocytes, especially in young rat group which impaired by H 2 O 2 -induced oxidative stress in vitro conditions. The results of this study first suggest that L-carnosine supplemention can be used to improve the RBC quality or to protect them from oxidative damage in survival of RBC in the circulation.

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Section: Discussionmentioning

confidence: 75%

Impaired erythrocytes deformability in H2O2-induced oxidative stress: Protective effect of L-carnosine

Aydoğan

Yapışlar²,

Artis³

et al. 2008

Clinical Hemorheology and Microcirculation

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“…Chronic ethanol consumption is associated with abnormal RBC morphology and an increased susceptibility to hemolysis (27,28). The underlying molecular mechanisms responsible for the effect of alcohol on RBC morphology are poorly understood (2)(3)(4)(5)(6)29).…”

Section: Resultsmentioning

confidence: 99%

Red blood cell fatty acid ethyl esters: a significant component of fatty acid ethyl esters in the blood

Best

Cluette‐Brown

Teruya

et al. 2003

Journal of Lipid Research

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An association between alcoholism and abnormal red blood cell (RBC) size and shape has long been recognized (1). However, the underlying pathophysiologic mechanisms responsible for the morphologic alterations are incompletely understood (2-6). Fatty acid ethyl esters (FAEEs), esterification products of ethanol and fatty acids, have been shown to be incorporated into phospholipid bilayers up to 30 mol % of total membrane fatty acids (7). FAEEs have been implicated as mediators of ethanolinduced organ damage (8-13) and have clinical utility as markers for ethanol intake (14, 15). Serum and plasma FAEE levels closely correlate with blood ethanol levels, but these circulating FAEEs in blood persist for at least 24 h after ethanol ingestion, long after ethanol is no longer detectable (15, 16).Although the largest reservoir of FAEE synthetic capability appears to reside in the pancreas and the liver (17, 18), enzyme activity that catalyzes FAEE synthesis has been detected in RBCs, white blood cells (WBCs), and platelets. Given the much higher concentration of RBCs in the blood, it has been suggested that RBCs may provide a significant portion of the total FAEE synthase activity in whole blood (19). With this information, the possibility that RBC membranes might synthesize and sequester FAEEs in the blood has been raised.We conducted a controlled clinical trial to determine if FAEEs accumulate and persist within RBCs following ethanol ingestion. The objectives of this study were to determine: 1 ) whether FAEEs appear in RBCs following ethanol ingestion and whether RBC-associated FAEEs correlate with blood ethanol concentration; 2 ) how long FAEEs persist in RBCs after ethanol intake is discontinued; 3 ) whether the fatty acid composition of RBC FAEEs is distinct from the fatty acid composition of FAEEs in plasma; and 4 ) whether RBC and plasma FAEE species undergo a remodeling process with changes in fatty acid composition following discontinuation of ethanol ingestion. METHODSThe study was approved by the institutional review board of the Massachusetts General Hospital. A written informed consent was obtained from each volunteer prior to subject enrollment.Abbreviations: AIC, akaike information criteria; E16:0, ethyl palmitate; E18:0, ethyl stearate; E18:1, ethyl oleate; E18:2, ethyl linoleate; FAEE, fatty acid ethyl ester; GC-MS, gas liquid chromatography-mass spectrometry; RBC, red blood cell; SPE, solid-phase extraction; WBC, white blood cell.

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“…In addition, even small concentration of H 2 O 2 generated during the autoxidation of oxyhemoglobin contributes to heme degradation. Heme degradation is, therefore, expected to take place in the erythrocyte when the antioxidant enzymes are not able to eliminate all the H 2 O 2 (Snyder et al, 1985;Prokopiva et al, 2000;Feher et al, 2006).…”

Section: Erythrocytes Oxidative Stress and Agingmentioning

confidence: 99%

Carnosine and Its Role on the Erythrocyte Rheology

Artis¹,

Aydoğan²

2012

Hemodynamics - New Diagnostic and Therapeutic Approaches

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Effects of Carnosine and Related Compounds on the Stability and Morphology of Erythrocytes From Alcoholics

Cited by 17 publications

References 22 publications

Impaired erythrocytes deformability in H2O2-induced oxidative stress: Protective effect of L-carnosine

Impaired erythrocytes deformability in H2O2-induced oxidative stress: Protective effect of L-carnosine

Red blood cell fatty acid ethyl esters: a significant component of fatty acid ethyl esters in the blood

Carnosine and Its Role on the Erythrocyte Rheology

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