Red blood cell fatty acid ethyl esters: a significant component of fatty acid ethyl esters in the blood

Best, Catherine A.; Cluette‐Brown, Joanne E.; Teruya, Miho; Teruya, Ami; Laposata, Michael

doi:10.1194/jlr.m200398-jlr200

Cited by 34 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike cholesterol which is evenly distributed between the inner and outer leaflets, the 5 major phospholipids are asymmetrically disposed (outer monolayer: phosphatidylcholine, PC and sphingomyelin, SM; inner monolayer: phosphatidylethanolamine, PE, phosphoinositol, PI and phosphatidylserine, PS) [17,37,38]. In the present study, concentrations of PUFA C18:2, C22:5 and C22:6 were decreased in membrane while concentrations of monounsaturated fatty acid (MUFA) C14:0 and C18:1 were dramatically increased, which resulted in a net higher concentration of total monounsaturated fatty acids in the positive control group [19,39]. Moreover, earlier studies also found that the carbonyls of C18:1 and C16:0 were exposed to the aqueous interface of phospholipids, the C18:1 and C16:0 on membranes may result in increase of rigid molecular order on membrane structures [19,39-42].…”

Section: Discussionmentioning

confidence: 68%

“…In the present study, concentrations of PUFA C18:2, C22:5 and C22:6 were decreased in membrane while concentrations of monounsaturated fatty acid (MUFA) C14:0 and C18:1 were dramatically increased, which resulted in a net higher concentration of total monounsaturated fatty acids in the positive control group [19,39]. Moreover, earlier studies also found that the carbonyls of C18:1 and C16:0 were exposed to the aqueous interface of phospholipids, the C18:1 and C16:0 on membranes may result in increase of rigid molecular order on membrane structures [19,39-42]. The worse partition of the fatty acid compositions in the lipid bilayer may alter the biochemical properties of the cell membrane, the membrane shape, organization, and permeability [10,39,41-46].…”

Section: Discussionmentioning

confidence: 78%

“…This phenomenon indicated that flaxseed oil contained a high content of n-3 PUFA and many double bonds in n-3 PUFA avoided damage on membrane form oxidative stress [18]. Membrane incorporation of PUFAs may reduce cellular susceptibility to lipid peroxidation [19], alter membrane fluidity [19], enhance receptor function, elevate enzyme activity [19,21] and influence the production of lipid mediators [19,21]. The observation was probably related to the reaction of free radicals with methylene groups in PUFA to avoid free radical conjugating with fatty acids of membrane [7,22,23].…”

Section: Discussionmentioning

confidence: 99%

“…Fatty acid compositions of erythrocytes membrane were detected by gas chromatography assay (GC) [19]. After incubation, the blood samples were washed three times by a 1-to-10 dilution with NaCl (0.15 M) before gas chromatography assay.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level

Yang

et al. 2012

Lipids Health Dis

View full text Add to dashboard Cite

BackgroundThe erythrocyte membrane lesion is a serious diabetic complication. A number of studies suggested that n-3 fatty acid could reduce lipid peroxidation and elevate α- or γ-tocopherol contents in membrane of erythrocytes. However, evidence regarding the protective effects of flaxseed oil, a natural product rich in n-3 fatty acid, on lipid peroxidation, antioxidative capacity and membrane deformation of erythrocytes exposed to high glucose is limited.MethodsHuman peripheral blood erythrocytes were isolated and treated with 50 mM glucose to mimic hyperglycemia in the absence or presence of three different doses of flaxseed oil (50, 100 or 200 μM) in the culture medium for 24 h. The malondialdehyde (MDA) and L-glutathione (GSH) were measured by HPLC and LC/MS respectively. The phospholipids symmetry and membrane fatty acid composition of human erythrocytes were detected by flow cytometry and gas chromatograph (GC). The morphology of human erythrocyte was illuminated by ultra scanning electron microscopy.ResultsFlaxseed oil attenuated hyperglycemia-induced increase of MDA and decrease of GSH in human erythrocytes. Human erythrocytes treated with flaxseed oil contained higher C22:5 and C22:6 than those in the 50 mM glucose control group, indicating that flaxseed oil could reduce lipid asymmetric distribution and membrane perturbation. The ultra scanning electron microscopy and flow cytometer have also indicated that flaxseed oil could protect the membrane of human erythrocytes from deformation at high glucose level.ConclusionThe flaxseed oil supplementation may prevent lipid peroxidation and membrane dysfunction of human erythrocytes in hyperglycemia.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level

Yang

et al. 2012

Lipids Health Dis

View full text Add to dashboard Cite

show abstract

“…The cells were washed 3 times with saline and suspended in phosphate buffered saline (pH 7.4), as described in Ref. [27]. The RBCs were then placed between cover slips and imaged without additional preparation.…”

mentioning

confidence: 99%

Optical Measurement of Cell Membrane Tension

et al. 2006

Self Cite

View full text Add to dashboard Cite

Using a novel noncontact technique based on optical interferometry, we quantify the nanoscale thermal fluctuations of red blood cells (RBCs) and giant unilamellar vesicles (GUVs). The measurements reveal a nonvanishing tension coefficient for RBCs, which increases as cells transition from a discocytic shape to a spherical shape. The tension coefficient measured for GUVs is, however, a factor of 4-24 smaller. By contrast, the bending moduli for cells and vesicles have similar values. This is consistent with the cytoskeleton confinement model, in which the cytoskeleton inhibits membrane fluctuations [Gov et al., Phys. Rev. Lett. 90, 228101, (2003) The red blood cell (RBC) has a composite membrane consisting of a lipid bilayer coupled to a two-dimensional spectrin network, which grants the cell its characteristic properties of both softness and strong shear elasticity [1,2]. Because mature RBCs lack nuclei, they represent a convenient model for studying cell membranes, which have broad applications in both science and technology [3,4]. The lipid bilayer is 4 -5 nm thick, and exhibits fluidlike behavior, characterized by a finite bending modulus and a vanishing shear modulus, 0. The resistance to shear, crucial for RBC function, is provided by the spectrin network, which has a mesh size of 80 nm.Spontaneous membrane fluctuations, or ''flickering,'' have been modeled theoretically under both static and dynamic conditions [5][6][7][8][9]. These thermally induced membrane motions exhibit 100 nm scale amplitudes at frequencies of tens of Hz. In past studies, measurements of the membrane mean squared displacement versus spatial wave vector, u 2 q, revealed a q ÿ4 dependence predicted by the equipartition theorem, which is indicative of fluidlike behavior [6,10 -13]. These results conflict with the static deformation measurements provided by micropipette aspiration [14,15], high-frequency electric fields [16,17], and, more recently, optical tweezers [18], which indicate an average value for the shear elasticity of the order of 10 ÿ6 J=m 2 . Gov et al. predicted that the cytoskeleton pinning of the membrane has an overall effect of confining the fluctuations and, thus, gives rise to superficial tension much larger than in the case of free bilayers [5]. This confinement model may offer new insight into the cytoskeleton-bilayer interaction that determines the morphology and physiology of the cell [19].RBCs can be assumed optically homogeneous. Therefore, measurement of the cell optical path length via interferometric techniques can provide information about the physical topography of the membrane with subwavelength accuracy and without contact. However, existing optical methods, including phase contrast microscopy (PCM) [6], reflection interference contrast microscopy (RICM) [10], and fluorescence interference contrast (FLIC) [20], are limited in their ability to measure cell membrane displacements. PCM provides phase shifts quantitatively only for samples that are optically much thinner than the wavelength of light, which ...

show abstract