Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro

Abstract

Effects of carboxymethyl-chitosan with different molecular weights on wound healing were investigated in vivo and in vitro. A second degree burn model was performed in rats and the accelerative effects of carboxymethyl-chitosan on wound repair were observed. Contents of transforming growth factor (TGF)-β1，interleukin(IL)-6 and matrix metalloproteinase (MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay (ELISA). In vitro study evaluated the influence of carboxymethyl-chitosan on cytokines secretion of fibroblasts and macrophages. In vivo results showed that carboxymethyl-chitosan effectively accelerated the wound healing process in burned rats (P<0.05). Levels of TGF-β1, IL-6 and MMP-1 in carboxymethyl-chitosan groups were significantly elevated, compared with control group (P<0.05). In vitro results indicated that carboxymethyl-chitosan significantly promoted the proliferation of fibroblasts and stimulated its IL-8 and IL-10 secretion at different incubation time, but it did not affect collagen secretion of fibroblasts. Carboxymethyl-chitosan enhanced phagocytosis ability of macrophages, and increased its tumor necrosis factor (TNF)-α secretion. In conclusion, carboxymethyl-chitosan promoted wound healing by activating macrophages, accelerating fibroblasts growth and exerting considerable effects on the secretion of a series of cytokines.

Long‐term antibiofilm activity of carboxymethyl chitosan on mixed biofilm on silicone

“…Several in vivo studies also have proved nontoxicity and good biocompatibility of CM‐chitosan . These beneficial biological properties make CM‐chitosan widely assessed in the biomedical material fields in vivo, such as drug delivery systems, plastic surgery, tissue engineering, and wound‐healing materials . The results of this study suggest that CM‐chitosan might offer a biocompatible and flexible platform for designing coatings to protect silicone surfaces from infection.…”

“…[33][34][35][36] These beneficial biological properties make CM-chitosan widely assessed in the biomedical material fields in vivo, such as drug delivery systems, 36 plastic surgery, 37 tissue engineering, 38 and wound-healing materials. 39 The results of this study suggest that CM-chitosan might offer a biocompatible and flexible platform for designing coatings to protect silicone surfaces from infection. Furthermore, recent studies showed that chitosan-coated surfaces have antibiofilm properties against bacteria and fungi in vitro.…”

Preparation and In Vitro Biological Evaluation of Lawsone Loaded O‐Carboxymethyl Chitosan/Zinc Oxide Nanocomposite for Wound‐Healing Application

“…Different types of wound healing scaffolds were prepared from naturally derived and synthetic polymers, of which the natural polymers serve better due to their unique biological adaptive properties . Nowadays, bio and synthetic polymer based wound dressings like nanocomposites, composite films, hydrogels, nanofiber mats with the incorporation of growth factors and drugs were fabricated . The attraction on biopolymers towards tissue engineering was owing to the biocompatible, bio‐degradation, non‐toxic and antimicrobial properties.…”

“…[5,6] Nowadays, bio and synthetic polymer based wound dressings like nanocomposites, composite films, hydrogels, nanofiber mats with the incorporation of growth factors and drugs were fabricated. [7,8] The attraction on biopolymers towards tissue engineering was owing to the biocompatible, bio-degradation, non-toxic and antimicrobial properties.…”

Preparation and characterization of gelatin/sericin/carboxymethyl chitosan medical tissue glue

“…Furthermore, it can promote the adhesion, growth and reproduction of fibroblasts in vitro (34) and in vivo (35). It can also promote skin wound healing (36). Scaffolds based on Gel and CMCS for dermal tissue engineering have been studied (37).…”