Preparation and In Vitro Biological Evaluation of Lawsone Loaded O‐Carboxymethyl Chitosan/Zinc Oxide Nanocomposite for Wound‐Healing Application

Abstract

A biopolymer based nanocomposite with O‐Carboxymethyl chitosan (O‐Cmc), nano zinc oxide (ZnO) and Lawsone (Ln) with proving advantages in antibacterial wound healing application was prepared. The physiochemical characteristics of the nanocomposite (NCs) was analysed by Fourier Transmission Infrared spectroscopy (FT‐IR), X‐Ray Diffractometer (XRD), Scanning electron Microscopy (SEM), Transmission electron microscope (TEM) and Thermogravimetric analysis (TGA). The biological properties such as anti‐inflammatory, anti‐oxidant activities were investigated in vitro. The nanocomposite also possesses efficient drug encapsulation and releasing profile with greater stability in phosphate buffer saline (PBS) and Dulbecco's minimal essential medium (DMEM). The results of the antibacterial studies show its enhanced bactericidal nature. The results obtained from MTT assay, Ethidium bromide stained fluorescence image and in vitro wound scratch assay show greater cell viability with the closure of scratch wound obviously proves it′s prominent biocompatible, wound closure nature. These biological properties show that the prepared nanocomposite has a greater potential to act as an excellent biomaterial in the field of wound care applications.

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“…The band near 820 cm −1 was related to the asymmetric deformation of CH3 vibrations (aromatic out-of-plain vibrations) [ 45 ]. Finally, the band at 1591cm −1 was due to C=C bands [ 46 ].…”

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“…Additionally, it demonstrated potent antibacterial activity and promoted cell viability and wound closure, highlighting its promising application in the field of wound care. [57] Also, a study focused on the development of a hemostaticantibacterial biomaterial incorporating lawsone for wound healing applications. By combining tempo-oxidized nanocellulose (TOCN), chitosan, and lawsone, the study aimed to create a scaffold capable of promoting hemostasis and wound closure.…”

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“…ZnO nanofillers also enhanced the antibacterial activity and keratinocyte migration of a polymer-matrix zinc oxide nanocomposite, leading to stronger antibacterial activity that prevented the reoccurrence of a bacterial infection and promoted healing [ 276 ]. Recent research has reported a prepared nanocomposite consisting of a Lawsone-loaded o-carboxymethyl chitosan and ZnO which was evaluated against bacterial strains such as Salmonella , S. aureus , P. aeruginosa , and E. coli [ 277 ]. This prepared nanocomposite tended to prevent the evolution of these harmful bacteria compared to an o-carboxymethyl chitosan or nano-zinc oxide alone, further supporting this advantageous strategy of using a polymer-matrix ZnO nanocomposite in wound dressings.…”