Effects of carbon dioxide anaesthesia on Drosophila melanogaster

Perron, J. M.; Huot, L.; Corrivault, G.-W.; Chawla, S. S.

doi:10.1016/0022-1910(72)90157-6

Cited by 42 publications

(26 citation statements)

References 10 publications

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“…This suggests that the effects of CO 2 are offset by a relatively short recovery time at room temperature, which is in contrast to the longer-term effects on fitness and behavior implied by previous studies (Perron et al, 1972;Barron, 2000). By contrast, the effects of N 2 on chill coma recovery decline, but persist after 90 min of recovery.…”

Section: Anesthetic Effects On Chill Coma Recoverymentioning

confidence: 60%

“…For anesthetics to be useful, it must be assumed that they have no effect on traits being measured in the laboratory. However, CO 2 exposure has been shown to reduce longevity, fecundity (Perron et al, 1972) and mating success (Barron, 2000) in D. melanogaster. Additionally, CO 2 anesthesia increased development time and impaired locomotion and feeding behavior in the German cockroach, Blattela germanica (Tanaka, 1982;Branscome et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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The effects of carbon dioxide anesthesia and anoxia on rapid cold-hardening and chill coma recovery in Drosophila melanogaster

Nilson

Sinclair

Roberts

2006

Journal of Insect Physiology

120

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Carbon dioxide gas is used as an insect anesthetic in many laboratories, despite recent studies which have shown that CO 2 can alter behavior and fitness. We examine the effects of CO 2 and anoxia (N 2 ) on cold tolerance, measuring the rapid cold-hardening (RCH) response and chill coma recovery in Drosophila melanogaster. Short exposures to CO 2 or N 2 do not significantly affect RCH, but 60 min of exposure negates RCH. Exposure to CO 2 anesthesia increases chill coma recovery time, but this effect disappears if the flies are given 90 min recovery in air before chill coma induction. Flies treated with N 2 show a similar pattern, but require significantly longer chill coma recovery times even after 90 min of recovery from anoxia. Our results suggest that CO 2 anesthesia is an acceptable way to manipulate flies before cold tolerance experiments (when using RCH or chill coma recovery as a measure), provided exposure duration is minimized and recovery is permitted before chill coma induction. However, we recommend that exposure to N 2 not be used as a method of anesthesia for chill coma studies.

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Section: Anesthetic Effects On Chill Coma Recoverymentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

The effects of carbon dioxide anesthesia and anoxia on rapid cold-hardening and chill coma recovery in Drosophila melanogaster

Nilson

Sinclair

Roberts

2006

Journal of Insect Physiology

120

View full text Add to dashboard Cite

show abstract

“…In general, this supports the idea that the effects of CO 2 anaesthesia are species specific and investigators should appropriately test the effect in their target species. Currently, the majority of work on the effect of CO 2 anaesthesia has been carried out on Drosophila melanogaster 5,11,22 and therefore may not be appropriate to apply to other related species.…”

Section: Discussionmentioning

confidence: 99%

“…A more realistic scenario is for a female to encounter multiple males 9,10 , allowing competition between males, which might allow the detection of more subtle losses of male fitness due to anaesthesia. The use of CO 2 anaesthesia has also been found to reduce fecundity and longevity of adult D. melanogaster when they are exposed shortly after eclosion, as is common when collecting virgin flies 11 . An alternative to CO 2 anaesthesia is to mark flies by feeding them food colored with dye 4,6,10,12,13 .…”

Section: Introductionmentioning

confidence: 99%

“…However, a paper directly examining the effects of the same food coloring on adult D. melanogaster found a strong reduction in lifespan 14 . Previous work has also focused almost entirely on D. melanogaster, both with regard to the effects of CO 2 anaesthesia 5,11 and food coloring 14 methods. Currently, there is little information on how CO 2 anaesthesia or the use of intestinal coloring affects the mating behavior of other Drosophila.…”

Section: Introductionmentioning

confidence: 99%

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Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized <em>Drosophila</em>

Verspoor

Heys

Price

2015

JoVE

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Mating experiments using Drosophila have contributed greatly to the understanding of sexual selection and behavior. Experiments often require simple, easy and cheap methods to distinguish between individuals in a trial. A standard technique for this is CO 2 anaesthesia and then labelling or wing clipping each fly. However, this is invasive and has been shown to affect behavior. Other techniques have used coloration to identify flies. This article presents a simple and non-invasive method for labelling Drosophila that allows them to be individually identified within experiments, using food coloring. This method is used in trials where two males compete to mate with a female. Dyeing allowed quick and easy identification. There was, however, some difference in the strength of the coloration across the three species tested. Data is presented showing the dye has a lower impact on mating behavior than CO 2 in Drosophila melanogaster. The impact of CO 2 anaesthesia is shown to depend on the species of Drosophila, with D. pseudoobscura and D. subobscura showing no impact, whereas D. melanogaster males had reduced mating success. The dye method presented is applicable to a wide range of experimental designs.

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CO₂ anesthesia on Drosophila survival in aging research

Shen

Yang

Zhu

et al. 2019

Arch Insect Biochem Physiol

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Carbon dioxide (CO 2 ) exposure is a common method of anesthesia in studies of Drosophila melanogaster. A number of negative side effects of CO 2 anesthesia have been reported.It is not clear whether the length of CO 2 anesthesia time affects Drosophila survival in aging research. Here, we examined the potential effect of the CO 2 anesthesia time length of 10-150 min. We found that long CO 2 exposure could lead to Drosophila death, more significant in males. The longer the anesthesia time is, the longer it takes for flies to wake up. Long-time CO 2 anesthesia can reduce the lifespan.Our stress tests showed that long-time CO 2 anesthesia can increase the average survival time in both males and females under starvation conditions, but can only increase female lifespan under H 2 O 2 oxidative stress. Long-time CO 2 anesthesia also significantly affects physiological traits, with spontaneous activity increased in females but decreased in males, and reduced female fecundity. Our study suggests that limiting the CO 2 anesthesia time and giving enough recovery time before performing physiological tests are important in Drosophila aging research.

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Effects of carbon dioxide anaesthesia on Drosophila melanogaster

Cited by 42 publications

References 10 publications

The effects of carbon dioxide anesthesia and anoxia on rapid cold-hardening and chill coma recovery in Drosophila melanogaster

The effects of carbon dioxide anesthesia and anoxia on rapid cold-hardening and chill coma recovery in Drosophila melanogaster

Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized <em>Drosophila</em>

CO₂ anesthesia on Drosophila survival in aging research

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Effects of carbon dioxide anaesthesia on Drosophila melanogaster

Cited by 42 publications

References 10 publications

The effects of carbon dioxide anesthesia and anoxia on rapid cold-hardening and chill coma recovery in Drosophila melanogaster

The effects of carbon dioxide anesthesia and anoxia on rapid cold-hardening and chill coma recovery in Drosophila melanogaster

Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized <em>Drosophila</em>

CO2 anesthesia on Drosophila survival in aging research

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CO₂ anesthesia on Drosophila survival in aging research