Effects of carbomer 940 hydrogel on burn wounds: an <i>in vitro</i> and <i>in vivo</i> study

Hayati, Farzad; Ghamsari, Seyed Mehdi; Dehghan, Mohammad Mehdi; Oryan, Ahmad

doi:10.1080/09546634.2018.1426823

Cited by 42 publications

(26 citation statements)

References 24 publications

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“…Carbomers, which are high molecular weight cross-linked polymers of acrylic acid, have been commonly used for the preparation of semi-solid or liquid formulations for topical administration (de Araújo Pereira and Bruschi, 2012; Berretta et al, 2013; Hayati et al, 2018). The carbomer used in this study (Carbopol ® 940) is composed of polyacrylic acid subunits highly cross-linked with pentaerythritol allyl ethers, and due to the presence of hydroxyl and carboxyl groups, it exhibits an anionic characteristic.…”

Section: Discussionmentioning

confidence: 99%

Hydrogel Containing Oleoresin From Copaifera officinalis Presents Antibacterial Activity Against Streptococcus agalactiae

et al. 2019

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Streptococcus agalactiae or Group B Streptococcus (GBS) remains a leading cause of neonatal infections worldwide; and the maternal vaginal-rectal colonization increases the risk of vertical transmission of GBS to neonates and development of infections. This study reports the in vitro antibacterial effect of the oleoresin from Copaifera officinalis Jacq. L. in natura (copaiba oil) and loaded into carbomer-hydrogel against planktonic and sessile cells of GBS. First, the naturally extracted copaiba oil was tested for the ability to inhibit the growth and metabolic activity of planktonic and sessile GBS cells. The time-kill kinetics showed that copaiba oil exhibited a dose-dependent bactericidal activity against planktonic GBS strains, including those resistant to erythromycin and/or clindamycin [minimal bactericidal concentration (MBC) ranged from 0.06 mg/mL to 0.12 mg/mL]. Copaiba oil did not inhibit the growth of different Lactobacillus species, the indigenous members of the human microbiota. The mass spectral analyses of copaiba oil showed the presence of diterpenes, and the kaurenoic acid appears to be one of the active components of oleoresin from C. officinalis related to antibacterial activity against GBS. Microscopy analyses of planktonic GBS cells treated with copaiba oil revealed morphological and ultrastructural alterations, displaying disruption of the cell wall, damaged cell membrane, decreased electron density of the cytoplasm, presence of intracellular condensed material, and asymmetric septa. Copaiba oil also exhibited antibacterial activity against established biofilms of GBS strains, inhibiting the viability of sessile cells. Low-cost and eco-friendly carbomer-based hydrogels containing copaiba oil (0.5% – CARB-CO 0.5; 1.0% – CARB-CO 1.0) were then developed. However, only CARB-CO 1.0 preserved the antibacterial activity of copaiba oil against GBS strains. This formulation was homogeneous, soft, exhibited a viscoelastic behavior, and showed good biocompatibility with murine vaginal mucosa. Moreover, CARB-CO 1.0 showed a slow and sustained release of the copaiba oil, killing the planktonic and sessile (established biofilm) cells and inhibiting the biofilm formation of GBS on pre-coated abiotic surface. These results indicate that carbomer-based hydrogels may be useful as topical systems for delivery of copaiba oil directly into de vaginal mucosa and controlling S. agalactiae colonization and infection.

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Section: Discussionmentioning

confidence: 99%

Hydrogel Containing Oleoresin From Copaifera officinalis Presents Antibacterial Activity Against Streptococcus agalactiae

et al. 2019

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“…Formed 3D scaffolds based on carbomer hydrogels can be used as transplants to study recovery processes at tissue injury in an in vivo experiments [19]. In general, the practical implications of this study will be the development of new biotechnological approaches for increasing effectiveness of adipose-derived MMSCs as an alternative source of adult stem cells in the injured ischemic tissue regeneration.…”

Section: а B Cmentioning

confidence: 99%

“…Carbomer or Carbopol is a polyacrylicacid polymer, which shows a sol to gel transition in aqueous solution as the pH is raised above its pKa of about 5.5 [18]. Due to their properties, carbomer hydrogels realize the potential of scaffolds of natural origin, providing a 3D structure similar to living tissue, while remaining non-toxic and non-immunogenic [19]. On the other hand, due to the ability to regulate the physical and chemical parameters of these hydrogels, changing their proportions and modifications of the components, the necessary kinetics of degradation of the scaffold is achieved, which correlates with the integration of the graft into the recipient's tissues [20].…”

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confidence: 99%

3D culture of murine adipose-derived multipotent mesenchymal stromal cells in hydrogel based on carbomer 974P

Кyryk¹,

Kuchuk²,

Mamchur³

et al. 2018

JCOT

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Actual issues during tissue regeneration are to ensure the survival of transplanted cells at the site of their application and further activity, especially in case of local pathological alterations such as inflammation and ischemia. For this purpose, the matrices that can not only fill the defects of tissues, but also be scaffolds for cells are developed.The aim of this study was to evaluate the effectiveness of 3D cultivation of murine adipose-derived multipotent mesenchymal stromal cells (MSMCs) in hydrogel based on carbomer 974P.Materials and methods. MSMCs were obtained from the adipose tissue of FVB-Cg-Tg(GFPU)5Nagy/J mice transgenic for GFP gene. The cells were phenotyped by flow cytometry and directly differentiated into osteogenic and adipogenic direction to confirm multipotent phenotype. MMSCs were cultured and directly differentiated into osteogenic direction in three-dimensional hydrogel scaffolds. For hydrogel preparation we used carbomer 974P with composition of glycerol, propylene glycol, triethylamine and agarose in original proportion.Results. The three-dimensional hydrogel based on carbomer 974P for the further engraftment with MMSCs was obtained. Modified protocols for the preparation of hydrogels based on carbomer and agarose and their rehydration by culture media for the 3D cultivation of adipose-derived MMSCs have been developed. The optimal concentration of MSMCs and the injection method for engraftment of hydrogels of the required form and size are selected. It was shown that adipose-derived MMSCs in 3D carbomer hydrogel preserve the potential of directed osteogenic differentiation.Conclusion. Three-dimensional hydrogel based on carbomer 974P is capable to support cells, provide the necessary cytoarchitectonics, maintain intercellular interactions, which can promote further long-term survival and specialization of graft.

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“…The inconvenience of this spray preparation included a short residence time on the skin and the requirement of frequent administration. Carbomer 940 hydrogel is a high molecular weight material with good biocompatibility that does not cause skin irritation (Sahoo et al, 2016;Cui et al, 2017;Hayati et al, 2018). Carbomer 940 hydrogel also provided favorable conditions for wound healing due to its high moisture retention, sustained drug release, and good adhesion (Wu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Toxicity Study of Topical Administration of a Highly-Stable rh-aFGF Carbomer 940 Hydrogel in a Rabbit Skin Wound Model

Zhang

Huang

et al. 2020

Front. Pharmacol.

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We developed a highly stable recombinant human acidic fibroblast growth factor (rh-aFGF) carbomer 940 hydrogel for wound healing. This study aimed to reveal toxicity target organs and the toxicity dose-response in the long-term administration of rh-aFGF carbomer 940 hydrogel in a rabbit skin wound model. New Zealand rabbits were topically administrated rh-aFGF carbomer 940 hydrogel at a daily dose of 900 IU/cm 2 , 1,800 IU/cm 2 , and 3,600 IU/cm 2 for 28 days. Lyophilized rh-aFGF agent was used as the positive control group. General behavior, serum chemistry, skin irritation, immunogenicity, immunotoxicity, and histopathology were analyzed at designated time points. Results revealed that food intake, body weight, body temperature, heart rate, and eye examinations were all normal, suggesting no obvious toxicity induced by the rh-aFGF hydrogel. Medium and high dose rh-aFGF hydrogel groups and the positive control group displayed increased cell numbers in the local lymph nodes near the site of administration, likely caused mesenteric lymph node follicular hyperplasia, and this observation was alleviated after 14 days of recovery. Immunogenicity studies demonstrated that the serum antibody titer against rh-aFGF increased with the duration and number of drug applications but were not neutralization antibodies. After administration stopped, antibody titer decreased and disappeared in some mice. In summary, the safe dose for long-term administration of rh-aFGF carbomer 940 hydrogel for persistently damaged skin was 900 IU/cm 2 , which is 10 times that of the proposed clinical dosing.

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Effects of carbomer 940 hydrogel on burn wounds: an in vitro and in vivo study

Abstract: Our study has shown that carbomer 940 hydrogel is a nontoxic biomaterial, which improved tissue perfusion and decreased the area of necrotic tissue in burn wounds. Application of this biomaterial in treatment of burn injuries deserves further studies.

Cited by 42 publications

References 24 publications

Hydrogel Containing Oleoresin From Copaifera officinalis Presents Antibacterial Activity Against Streptococcus agalactiae

Hydrogel Containing Oleoresin From Copaifera officinalis Presents Antibacterial Activity Against Streptococcus agalactiae

3D culture of murine adipose-derived multipotent mesenchymal stromal cells in hydrogel based on carbomer 974P

Long-Term Toxicity Study of Topical Administration of a Highly-Stable rh-aFGF Carbomer 940 Hydrogel in a Rabbit Skin Wound Model

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