Effects of CAMP and cGMP Elevating Agents on HL‐60 Cell Differentiation

Be, Bang; Ericsen, C; Aarbakke, Jarle

doi:10.1111/j.1600-0773.1994.tb00331.x

Cited by 18 publications

(16 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such a causal relationship between differentiation and increased cAMP levels were already shown of phosphodiesterase inhibitors (Chaplinski & Niedel 1982;Elks & Manganiello 1985;Bang et al 1994). However, in the present study, although glycoprotein Ib decreased expression and cAMP increases varied in the same direction, there was no quantitative relationship between the two parameters.…”

Section: Discussionsupporting

confidence: 59%

“…However, in the present study, although glycoprotein Ib decreased expression and cAMP increases varied in the same direction, there was no quantitative relationship between the two parameters. For example, the decrease of glycoprotein Ib expression was of the same extent for milrinone, RO-201724 and 3-isobutyl-1-methylxanthine (100 pM) whereas the amount of cAMP produced by these agents on Dami cells were 41, 4 and 15 pmol/106 cells respectively (Zurbonsen et al 1997a) suggesting that factors other than cAMP could also be implicated and in particular cGMP Differentiating effects of cGMP have indeed been described previously (Boss 1989;Bang et al 1994). In the present study cGMP also appears to be implicated in glycoprotein Ib decreased expression: on one hand Zaprinast, a cGMP phosphodiesterase type V inhibitor and 8-bromo cGMP were capable of inducing such effect and on the other hand SCA40, SCA41, SCA44 and IBMX elicited inhibitory effects on phosphodiesterase type V isolated from Dami cells (Zurbonsen el al.…”

Section: Discussionsupporting

confidence: 55%

“…Cyclic nucleotides are known to be involved in cell differentiation (Cho-Chung 1990;Boss 1989;Bang et al 1994). The modulation of their intracellular contents by selective and non-selective phosphodiesterase inhibitors were reported to induce cell differentiation (Chaplinski & Niedel 1982;Elks & Manganiello 1985;Boss 1989;Bang et al 1994).…”

mentioning

confidence: 99%

“…The modulation of their intracellular contents by selective and non-selective phosphodiesterase inhibitors were reported to induce cell differentiation (Chaplinski & Niedel 1982;Elks & Manganiello 1985;Boss 1989;Bang et al 1994). However, although numerous new phosphodiesterase inhibitors have been synthesised (Beavo & Reifsnyder 1990) and tested on cell growth, few studies have been realised concerning their effect on cell differentiation.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Modulation of the Megakaryoblastic Dami Cell Line Differentiation by Phosphodiesterase Inhibitors and Imidazo[1,2‐a]pyrazine Derivatives

Zurbonsen

Michel²,

Vittet

et al. 1997

Pharmacology & Toxicology

View full text Add to dashboard Cite

Phosphodiesterase inhibitors have been shown to modulate cell differentiation. We have previously shown that a series of imidazo[ 1,2-a]pyrazine derivatives displayed inhibitory effects on phosphodiesterase isoenzymes types 111, IV and V isolated from Dami cells and on Dami cell growth. In the present study we have investigated the effect of these derivatives on the expression of two differentiation markers, glycoproteins Ib and IIb/IIIa of the human megakaryoblastic leukaemic Dami cell line in comparison to those elicited by 3-isobutyl-1-methylxanthine and selective phosphodiesterase inhibitors of types I (8-methoxymethyl-l-methyl-3-(2-methylpropyl) xanthine), 111 (Mikinone), IV (RO-201724) and V (Zaprinast). Imidazo[l,2-a]pyrazine derivatives, 3-isobutyl-1 -methylxanthine and selective phosphodiesterase inhibitors, except 8-methoxymethyl-l-methyl-3-(2-methylpropyl) xanthine, decreased glycoprotein Ib expression. SCA40, SCA41, SCA44 and 3-isobutyl-1-methylxanthine but not the other compounds affected the expression of glycoprotein IIb/IIIa in a positive manner. The effects of imidazo [ 1,2-u]pyrazine derivatives on glycoprotein expression appeared to be related to their phosphodiesterase inhibitory potency.Cyclic nucleotides are known to be involved in cell differentiation ( Materials and MethodsMaterials. Dami cells were obtained from the American Type Culture Collection (Rockville, MD, U.S.A.). Horse serum was purchased from Techgen International (Les Ulis, France), and cell culture medium and reagents were from Gibco BRL (Eragny, France).Monoclonal antibodies P2 anti-glycoprotein IIb/IIIa (CD4la) and SZ2 anti-glycoprotein Ib (CD42b) were obtained from Immunotech (Marseilles, France) and the FITC-labeled goat rabbit anti-mouse immunoglobuline f(ab'), from Dako SA (Versailles, France). Milrinone (1,6-dihydro-2-mCthyl-6-0~0-(3,4'-bipyridine)-5-carbonitrile), RO-201724 amino)imidazo[l,2-a]pyrazine-2-carbonitrile), SCA44 (6-bromo-8-(cyclopen tylamino)imidazo[ 1,2-a]pyrazine-2-carboni trile) were synthesised in our laboratory as previously described (Bonnet et al. 1992). Other reagents were from Sigma (St. Quentin Fallavier, France).Cell culture conditions. Cells were grown in RPMI 1640 medium supplemented with 10% heat inactivated horse serum, 2 mM gluta-

show abstract

Section: Discussionsupporting

confidence: 59%

Section: Discussionsupporting

confidence: 55%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Modulation of the Megakaryoblastic Dami Cell Line Differentiation by Phosphodiesterase Inhibitors and Imidazo[1,2‐a]pyrazine Derivatives

Zurbonsen

Michel²,

Vittet

et al. 1997

Pharmacology & Toxicology

View full text Add to dashboard Cite

show abstract

“…2A; Table 2) and late expression in embryogenesis (Table 2). Members of the PDE family regulate tissue-specific growth and differentiation (3,15,63).…”

Section: Identification Of 12 Genes Transcriptionally Activated Bymentioning

confidence: 99%

E2a-Pbx1 Induces Aberrant Expression of Tissue-Specific and Developmentally Regulated Genes When Expressed in NIH 3T3 Fibroblasts

Kamps

1997

Molecular and Cellular Biology

100

View full text Add to dashboard Cite

The E2a-Pbx1 oncoprotein contains the transactivation domain of E2a joined to the DNA-binding homeodomain (HD) of Pbx1. In mice, E2a-Pbx1 transforms T lymphoblasts and fibroblasts and blocks myeloblast differentiation. Pbx1 and E2a-Pbx1 bind DNA as heterodimers with other HD proteins whose expression is tissue specific. While the transactivation domain of E2a is required for all forms of transformation, DNA binding by the Pbx1 HD is essential for blocking myeloblast differentiation but dispensable for fibroblast or T-lymphoblast transformation. These properties suggest (i) that E2a-Pbx1 causes cellular transformation by activating gene transcription, (ii) that transcription of E2a-Pbx1 target genes is normally regulated by ubiquitous Pbx proteins and tissue-specific partners, and (iii) that DNA-binding mutants of E2a-Pbx1 activate a subset of all gene targets. To test these predictions, genes induced in NIH 3T3 fibroblasts by E2a-Pbx1 were identified and examined for tissue-and stage-specific expression and their differential abilities to be upregulated by E2a-Pbx1 in NIH 3T3 fibroblasts and myeloblasts and by a DNA-binding mutant of E2a-Pbx1 in NIH 3T3 cells. Of 12 RNAs induced by E2a-Pbx1, 4 encoded known proteins (a J-C region of the immunoglobulin kappa light chain, natriuretic peptide receptor C, mitochondrial fumarase, and the 3,5-cyclic nucleotide phosphodiesterase, PDE1A) and 5 encoded new proteins related to angiogenin, ion channels, villin, epidermal growth factor repeat proteins, and the human 2.19 gene product. Expression of many of these genes was tissue specific or developmentally regulated, and most were not expressed in fibroblasts, indicating that E2a-Pbx1 can induce ectopic expression of genes associated with lineage-specific differentiation.

show abstract

Establishment and characterization of adenoviral E1A immortalized cell lines derived from the rat suprachiasmatic nucleus

et al. 1999

View full text Add to dashboard Cite

Primary cultured cells from the presumptive anlage of the rat suprachiasmatic nucleus (SCN) were immortalized by infection with a retroviral vector encoding the adenovirus 12S E1A gene. After drug selection, the resulting neural cell lines (SCN1.4 and SCN2.2) displayed (a) extended growth potential without evidence of transformed or tumorigenic properties, (b) expression of E1A protein within all cell nuclei, and (c) heterogeneous cell types in various stages of differentiation. A large proportion of the SCN1.4 and SCN2.2 cells were characterized by gliallike morphologies, but showed limited expression of corresponding cell type–specific antigens. In addition, both lines exhibited a stable population of cells with neuronlike characteristics. When treated so as to enhance differentiation, these cells were often distinguished by fine, long processes and immunocytochemical expression of neuronal markers and peptides found within SCN neurons in situ. Observations on SCN neuropeptide immunostaining, content, release, and mRNA expression followed a concordant pattern in which somatostatin and vasopressin cells were the most and least common peptidergic phenotypes in both lines, respectively. Since these results indicate that constituents of E1A‐immortalized lines derived from the primordial SCN can differentiate into cells with phenotypes resembling parental peptidergic neurons, it will be critical to explore next whether these lines also retain the distinctive function of the SCN to generate circadian rhythms. Cloning of immortalized cell types could subsequently yield useful tools for studying the development of SCN glial and peptidergic cell types and delineating their distinct roles in mammalian circadian timekeeping. © 1999 John Wiley & Sons, Inc. J Neurobiol 39: 1–13, 1999

show abstract

Effects of CAMP and cGMP Elevating Agents on HL‐60 Cell Differentiation

Cited by 18 publications

References 16 publications

Modulation of the Megakaryoblastic Dami Cell Line Differentiation by Phosphodiesterase Inhibitors and Imidazo[1,2‐a]pyrazine Derivatives

Modulation of the Megakaryoblastic Dami Cell Line Differentiation by Phosphodiesterase Inhibitors and Imidazo[1,2‐a]pyrazine Derivatives

E2a-Pbx1 Induces Aberrant Expression of Tissue-Specific and Developmentally Regulated Genes When Expressed in NIH 3T3 Fibroblasts

Establishment and characterization of adenoviral E1A immortalized cell lines derived from the rat suprachiasmatic nucleus

Contact Info

Product

Resources

About