Effects of caffeic acid phenethyl ester in reducing cerebral edema in rat subjects experiencing brain injury: An in vivo study

Abstract

Background A head injury is a very dangerous condition that threatens human life. This study examines the use of caffeic acid phenethyl ester (CAPE) in reducing cerebral edema in cases of head injury. The purpose of this study is to demonstrate whether CAPE can improve various parameters related to the expression of Aquaporin-4 (AQP4) mRNA and the serum AQP4 levels in rat subjects. Methods This is a randomized controlled study using a posttest-only control group design that uses experimental animals—specifically, male Rattus norvegicus ( Sprague Dawley strain) rats aged 10–12 weeks and weighing 200–300 g. This study used a head injury model according to Marmarou (1994) with minor modifications to the animal model fixation tool. The parameters of the AQP4 mRNA were examined with real-time PCR, while serum AQP4 levels were examined with sandwich ELISA. Results The AQP4 mRNA expression in rats that were given CAPE was lower than those not given CAPE, both on the fourth and seventh days; serum AQP4 levels in rats that were given CAPE were also lower than those not given CAPE, both on the fourth and seventh days. Conclusion Administration of CAPE in a rat model with head injury can reduce cerebral edema, mediated by AQP4.

Role of MLC901 in increasing neurogenesis in rats with traumatic brain injury

“…Brain injury is the leading cause of death from accidents under the age of 40. Each year around 10 million people worldwide are hospitalized for brain injuries [ 17 , 18 ].…”

Investigation of the anti-inflammatory effects of caffeic acid phenethyl ester in a model of λ-Carrageenan–induced paw edema in rats

“…Carr + CAPE 10 Group: One hour after administering CAPE (10 mg/kg body weight) intraperitoneally, 0.1 mL of 1% Carr was injected intraplantarly into the right hind paw. 15 5. Carr + CAPE 30 Group: One hour after administering CAPE (30 mg/kg body weight) intraperitoneally, 0.1 mL of 1% Carr was injected intraplantarly into the right hind paw.…”

“…Carr + CAPE 10 Group: One hour after administering CAPE (10 mg/kg body weight) intraperitoneally, 0.1 mL of 1% Carr was injected intraplantarly into the right hind paw. 15…”

“…Doses of Carr, Ind, and CAPE were determined with reference to previous studies. 7,[14][15][16] The groups were designed as given below;…”

Effects of hyperbaric oxygen therapy on the healing of thermal burns and its relationship with ICAM-1: A case-control study

“…Meanwhile, the nucleotide sequences for 18SRib as the housekeeping gene were F: 5′-GTAACCCGTTGAACCCCATT-3′ and R: 5′-CCATCCAATCGGTAGTAGCG-3′ (Macrogen, Inc., South Korea). mRNA was isolated using the RNeasy Mini Kit (74,106; QIAGEN, USA), according to the manufacturer's instructions [ [14] , [15] , [16] , [17] ]. The mRNA was reverse-transcribed using SuperScript III (18,080–051; Invitrogen, USA) with random hexamer primers.…”