Effects of cadmium on the gene transcription of the liver in the freshwater turtle (Chinemys reevesii)

Huo, Junfeng; Dong, A.; Yan, Jing; Dong, Ailing

doi:10.1007/s11356-019-07432-9

Cited by 15 publications

(3 citation statements)

References 61 publications

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“…One such important environmental pollutant in freshwater ecosystems is cadmium which can be ingested by turtles. Cadmium affects turtles' hepatic enzyme levels, gene expression, and DNA methylation, thereby exhibiting toxic damage to the liver (Huo et al, 2020a(Huo et al, , 2020bMizoguchi et al, 2022). Therefore, turtle-derived hepatic organoids could be a valuable model for studying aquatic ecosystem health and further cellular and molecular effects of heavy metal pollution on endangered and non-vulnerable species alike.…”

Section: Benefits Of 3d Organoids For Conservation Ecotoxicology and ...mentioning

confidence: 99%

Characterization of the First Turtle Organoids: A Model for Investigating Unique Adaptations with Biomedical Potential

Zdyrski

Gabriel

Gessler

et al. 2023

Preprint

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Painted turtles are remarkable for their well-developed freeze tolerance and associated resilience to hypoxia/anoxia, oxidative stress, and ability to supercool. They are, therefore, are an ideal model for biomedical research on hypoxia-induced injuries (such as strokes), tissue cooling during extensive surgeries, and organ cryopreservation. Yet, the seasonal reproduction and slow maturation of turtles hinder basic and applied biomedical research. To overcome these limitations, we have developed for the first time, adult stem cell-derived turtle hepatic organoids, which provide 3D self-assembled structures that closely mimic their original tissue and allow for in vitro testing and experimentation without constantly harvesting donor tissue and screening offspring. Our pioneering work with turtles represents the first for this vertebrate Order and complements the only other organoid lines from non-avian reptiles, which were produced from snake venom glands. Here we report the isolation and characterization of hepatic organoids derived from painted, snapping, and spiny softshell turtles spanning ~175 million years of evolution, with a subset being preserved in a biobank. Morphological and transcriptomics revealed organoid cells resembling cholangiocytes, which was then compared to the tissue of origin. Deriving organoids from multiple turtle species and life stages demonstrates that our organoid culturing techniques are broadly applicable to chelonians, permitting the development of functional genomic tools currently missing in most herpetological research. When combined with genetic editing, this platform will further support studies of genome-to-phenome mapping, gene function, genome architecture, and adaptive responses to climate change, among others. We discuss the unique abilities of painted turtles, including their overwintering potential, an adaptation with important implications for ecological, evolutionary, and biomedical research.

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Section: Benefits Of 3d Organoids For Conservation Ecotoxicology and ...mentioning

confidence: 99%

Characterization of the First Turtle Organoids: A Model for Investigating Unique Adaptations with Biomedical Potential

Zdyrski

Gabriel

Gessler

et al. 2023

Preprint

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“…In recent years, the C. reevesii has become a fast-rising wave of breeding for more and more turtle farmers. At the same time, a series of breeding models have been established, and a large number of large-scale breeding farms have been formed, of which pond and greenhouse cultivation have become the mainstream and the largest breeding modes [8][9][10]. With the expansion of breeding density, the breeding environment has been under tremendous pressure, which has led to the deterioration of farming water quality, disease outbreaks, drug abuse, and has caused huge losses to the turtle industry as well threatened food quality and safety and human health.…”

Section: Introductionmentioning

confidence: 99%

A First Insight into the Structural and Functional Comparison of Environmental Microbiota in Freshwater Turtle Chinemys reevesii at Different Growth Stages under Pond and Greenhouse Cultivation

et al. 2020

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The microbial community structure of water is an important indicator for evaluating the water quality of the aquaculture environment. In this study, the investigation and comparison of the bacterial communities of pond cultivation (PC) and greenhouse cultivation (GC) between hatchling, juvenile, and adult growth stages of C. reevesii were performed. In addition, the V4 regions of the 16S rRNA gene were sequenced. The Chao1 richness estimator of the PC group was significantly higher than that of the GC group. The beta diversity showed that the microbiotas of the two groups were isolated from each other. The dominant phyla were Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Verrucomicrobia, and Planctomycetes in the PC group and Proteobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, Chloroflexi, and Actinobacteria in the GC group. Both the numbers and the types of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotations differed between the PC and GC groups. The prediction of bacterial phenotype implied that the GC environment is more likely to deteriorate, and turtles are more susceptible to pathogens than those of the PC environment. In addition, a total of nine potential pathogenic bacteria were identified and the correlation of environmental factors analyses showed significant differences of bacterial species between the PC and GC groups, while the potential pathogenic bacteria showed significant correlation with the stocking density, temperature, pH, orthophosphate (PO4-P), and dissolved oxygen (DO) in both the PC and GC groups. Noticeably, this is the first report to describe the different microbiota characteristics of the different cultivation environments in the different growth stages of C. reevesii, which will provide valuable data for water quality adjustment, disease prevention, and the healthy breeding of turtles.

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“…In recent years, the C. reevesii has become a fast-rising wave of breeding for more and more turtle farmers. At the same time, a series of breeding models have been established, and a large number of large-scale breeding farms have been formed, of which pond and greenhouse cultivation have become the mainstream and the largest breeding modes (Huo et al, 2017(Huo et al, , 2020Min et al, 2019). With the expansion of breeding density, the breeding environment has been under tremendous pressure, which has led to the deterioration of farming water quality, disease outbreaks, drug abuse, and has caused huge losses to the turtle industry as well threatened food quality and safety and human health.…”

Section: Introductionmentioning

confidence: 99%

Comparing environmental microbiota of different growth stages of fresh water turtle Chinemys reevesii in pond and greenhouse cultivation

Zhou¹,

Xie²,

Sun³

et al. 2020

Preprint

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The microbial community structure is an important indicator for evaluating the water quality of the aquaculture environment. In this study, V4 regions of 16S rRNA gene of pond (PC) and greenhouse cultured (GC) C. reevesii were sequenced. Results showed that a total of 1,993,090 high quality counts and 105,159 observed OUTs were obtained; and the Chao1 richness estimator of PC was significantly higher than that of GC groups. Beta-diversity showed that the microbiota of two groups were isolated from each other. In addition, the correlation analysis of environmental factors showed that NO2-N, PH, PO4-P, and stocking density played significant roles in the bacterial community composition. The dominant phyla in PC groups were cyanobacteria, proteobacteria, actinobacteria, bacteroidetes, verrucomicrobia, planctomycetes; and in GC groups were proteobacteria, bacteroidetes, firmicutes, cyanobacteria, chloroﬂexi, actinobacteria. The functional prediction showed that the top5 Picrust prediction gene functions were protein processing in endoplasmic reticulum, retinol metabolism, proteasome, glycan binding proteins, and stilbenoid, diarylheptanoid and gingerol biosynthesis. Meanwhile, the numbers and types of KEGG pathway annotations showed a significant difference between the two cultivation environments. The prediction of bacterial phenotype implied that the GC environment is more likely to deteriorate, and turtles are more susceptible to pathogens than those of PC environment. This is the first report to explore and understand the difference of microbiota characteristics between different cultivation environments in different growth stages of C. reevesii, which will provide basic data for water quality adjustment, disease prevention, and healthy breeding of turtle.

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Effects of cadmium on the gene transcription of the liver in the freshwater turtle (Chinemys reevesii)

Cited by 15 publications

References 61 publications

Characterization of the First Turtle Organoids: A Model for Investigating Unique Adaptations with Biomedical Potential

Characterization of the First Turtle Organoids: A Model for Investigating Unique Adaptations with Biomedical Potential

A First Insight into the Structural and Functional Comparison of Environmental Microbiota in Freshwater Turtle Chinemys reevesii at Different Growth Stages under Pond and Greenhouse Cultivation

Comparing environmental microbiota of different growth stages of fresh water turtle Chinemys reevesii in pond and greenhouse cultivation

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