Effects of C18 Fatty Acids on Intracellular Ca²⁺Mobilization and Histamine Release in RBL-2H3 Cells

Abstract

To investigate the underlying mechanisms of C18 fatty acids (stearic acid, oleic acid, linoleic acid and α-linolenic acid) on mast cells, we measured the effect of C18 fatty acids on intracellular Ca2+ mobilization and histamine release in RBL-2H3 mast cells. Stearic acid rapidly increased initial peak of intracellular Ca2+ mobilization, whereas linoleic acid and α-linolenic acid gradually increased this mobilization. In the absence of extracellular Ca2+, stearic acid (100 µM) did not cause any increase of intracellular Ca2+ mobilization. Both linoleic acid and α-linolenic acid increased intracellular Ca2+ mobilization, but the increase was smaller than that in the presence of extracellular Ca2+. These results suggest that C18 fatty acid-induced intracellular Ca2+ mobilization is mainly dependent on extracellular Ca2+ influx. Verapamil dose-dependently inhibited stearic acid-induced intracellular Ca2+ mobilization, but did not affect both linoleic acid and α-linolenic acid-induced intracellular Ca2+ mobilization. These data suggest that the underlying mechanism of stearic acid, linoleic acid and α-linolenic acid on intracellular Ca2+ mobilization may differ. Linoleic acid and α-linolenic acid significantly increased histamine release. Linoleic acid (C18:2: ω-6)-induced intracellular Ca2+ mobilization and histamine release were more prominent than α-linolenic acid (C18:3: ω-3). These data support the view that the intake of more α-linolenic acid than linoleic acid is useful in preventing inflammation.

Brain Factor-7® improves learning and memory deficits and attenuates ischemic brain damage by reduction of ROS generation in stroke in vivo and in vitro

“…According to the previous study [ 26 ], Human neuroblastoma SH-SY5Y cells were cultured at 37 °C in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS which was inactivated by heat in a humidified 95% air, 5% CO 2 incubator. The cells were transferred to low serum media (10% FBS) for two hours before the treatment with BF-7.…”

In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds

“…Oleic acid ( 6 ) was reported to weakly inhibit histamine and beta-hexosaminidase release by antigen- and A23187-induced RBL-2H3 cells [47, 48]. In addition, oleic acid only slightly increased intracellular Ca 2+ that induced cell degranulation [49]. In an in vivo study, peanuts containing high oleic acid was also found to be less allergenic than normal peanuts [50].…”