In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds

Abstract

BackgroundMoringa oleifera Lam. is a commonly used plant in herbal medicine and has various reported bioactivities such as antioxidant, antimicrobial, anticancer and antidiabetes. It is rich in nutrients and polyphenols. The plant also has been traditionally used for alleviating allergic conditions. This study was aimed to examine the anti-allergic activity of M. oleifera extracts and its isolated compounds.MethodM. oleifera leaves, seeds and pods were extracted with 80% of ethanol. Individual compounds were isolated using a column chromatographic technique and elucidated based on the nuclear magnetic resonance (NMR) and electrospray ionisation mass spectrometry (ESIMS) spectral data. The anti-allergic activity of the extracts, isolated compounds and ketotifen fumarate as a positive control was evaluated using rat basophilic leukaemia (RBL-2H3) cells for early and late phases of allergic reactions. The early phase was determined based on the inhibition of beta-hexosaminidase and histamine release; while the late phase was based on the inhibition of interleukin (IL-4) and tumour necrosis factor (TNF-α) release.ResultsTwo new compounds; ethyl-(E)–undec-6-enoate (1) and 3,5,6-trihydroxy-2-(2,3,4,5,6-pentahydroxyphenyl)-4H-chromen-4-one (2) together with six known compounds; quercetin (3), kaempferol (4), β-sitosterol-3-O-glucoside (5), oleic acid (6), glucomoringin (7), 2,3,4-trihydroxybenzaldehyde (8) and stigmasterol (9) were isolated from M. oleifera extracts. All extracts and the isolated compounds inhibited mast cell degranulation by inhibiting beta-hexosaminidase and histamine release, as well as the release of IL-4 and TNF-α at varying levels compared with ketotifen fumarate.ConclusionThe study suggested that M. oleifera and its isolated compounds potentially have an anti-allergic activity by inhibiting both early and late phases of allergic reactions.

Mechanistic Studies of the Antiallergic Activity of Phyllanthus amarus Schum. & Thonn. and Its Compounds

“…The bioassay was conducted as reported by Abd Rani, et al [ 35 ]. In the concentration of 2 × 10 5 cells in 400 µL per well, RBL-2H3 cells were seeded and incubated for 2 h. After 2 h, the cells were sensitized with anti-DNP IgE at a concentration of 0.45 µg/mL in 100 µL per well.…”

“…The bioassay was conducted as reported by Abd Rani, et al [ 35 ]. RBL-2H3 cells (5 × 10 6 cells) in 10 mL of PBS buffer were sonicated for 20 min.…”

“…The bioassay was conducted as reported by Shahari, et al [ 36 ] and Abd Rani, et al [ 35 ]. In 24-well plates, 2 × 10 5 of RBL-2H3 cells were seeded in 400 µL per well.…”

In silico screening of antiviral compounds from Moringa oleifera for inhibition of SARS-CoV-2 main protease

“…A total of 216 phytochemical compounds from M. oleifera were obtained from the literature ( Table S1 ) [ 11 , 17 , 24 , 25 ].The 3D structures of the compounds chosen from M. oleifera [ 26 ]were acquired from ChemSpider ( www.chemspider.com ) [ 27 ], PubChem ( https://pubchem.ncbi.nlm.nih.gov/ ) [ 28 ] and ChEMBL ( https://www.ebi.ac.uk/chembl/ ) databases [ 29 ]. The structures of the target main protease (M pro ) (PDB ID: 6LU7 ) ( Fig.…”

Antiviral perspectives of economically important Indian medicinal plants and spices

“…Two nucleoside analogs and one protease inhibitor. It has immune potentiating effects by increasing leukocyte count and T-cell differentiation Bhuiyan et al ( 2020 ) SARS-CoV-2 NSP15 Blocks NF-kB transcription, which increases cytokines and other growth-promoting factors during inflammation, inhibits JAK/STAT signaling, which blocks anti-apoptotic, and proteasome-mediated degradation complex inhibition, which blocks apoptotic cell death Wadhwa et al ( 2017a ) Other Uses Treating neurological disorder, a stress reliever and Parkinson’s, Huntington’s, Alzheimer’s diseases Dhanani et al ( 2017 ), Marslin et al ( 2015 ), Wadhwa et al ( 2017b ) 4 Moringa oleifera Leaf Aqueous extract Herpex simplex virus (HSV-1 and HSV-2), HBV-C DNA Beta-amyrin and flavonoid Nasr-Eldin et al ( 2018 ), Feustel et al ( 2017 ) Methanol extract Epstein Barr virus Glycoside Murakami et al, 1(998 ) Aqueous extract Poliovirus Flavonoid quercetin reducing the overexpression of pirin protein Neznanov et al ( 2008 ) Seed Other Uses Potential to lower the serum cholesterol Barbosa et al ( 2019 ) Seed Other Uses Inhibitory activity on beta-hexosaminidase and TNF-α release Abd Rani et al ( 2019 …”