Effects of C‐Terminal Amelogenin Peptide on Proliferation of Human Cementoblast Lineage Cells

Abstract

C-terminal amelogenin cleavage product increased proliferation of HCEM through MAPK-ERK signaling pathway, indicating possible application of C11 peptide for periodontal tissue regeneration.

The C‐terminus of the amelogenin peptide influences the proliferation of human bone marrow mesenchymal stem cells

“…Therefore, in the current study, a peptide consisting of the 11 C‐terminal amino acids of amelogenin, termed rh174, was studied. C‐terminal amelogenins promote the metabolism of human cementoblast lineage cells . Full‐length amelogenin and two splice variants of amelogenin (M73 [LRAP + exon4] and M59 [LRAP]) interact with LAMP1 .…”

“…AMG‐CP was derived from human full‐length amelogenin and contains 11 amino acids of the C‐terminal hydrophilic region. AMG‐CP was industrially synthesized and purified as described previously …”

“…Previously studied were the effects of human full‐length amelogenin (rh174) on periodontal cells, and it was shown that rh174 increases the metabolism of human cementoblasts, human periodontal ligament cells, and human MSCs and regulates proliferation and differentiation of these cell types . Various fragments of amelogenins are present in vivo due to proteolytic degradation . Amelogenins are polar molecules, with a hydrophobic N‐terminus and a hydrophilic C‐terminus.…”

“…Amelogenins are polar molecules, with a hydrophobic N‐terminus and a hydrophilic C‐terminus. In physiologic conditions, this polarity induces aggregation of amelogenins in solution and subsequent lower activity . Thus, amelogenins may not be able to induce cell activation in vivo.…”

“…Increased safety and development of effective therapies may be possible with the use of trace amounts of amelogenin peptide. A previous study showed that the C‐terminus of amelogenin contains the active site and that a synthetic amelogenin peptide plays a role in the metabolism of human cementoblast lineage cells …”