Effects of C. parvum on growth and induction of intracerebral tumours in mice

Osborn, Djoah; Sadler, T E; Castro, J. E.

doi:10.1038/bjc.1977.63

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…Our observation that only low levels of activity were present in brains after injection of labelled C. parvum correlates well with the finding that C. parvum has only slight inhibitory effects on the growth and induction of experimental intracerebral tumours (Osborn, Sadler and Castro, 1977). This result was not unexpected, as the blood-brain barrier allows only a poor immunological response to antigen within the brain (Holman, 1972;Medawar, 1948;Denlinger et at., 1975).…”

Section: Discussionsupporting

confidence: 88%

Radiolabelling of Corynebacterium parvum and its distribution in mice

Sadler¹,

Cramp²,

Castro³

1977

Br J Cancer

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Corynebacterium parvum was labelled by growing live bacteria in the presence of [3H]thymidine. The bacteria were killed by formalin, washed thoroughly and resuspended at a concentration of 7 mg dry weight/ml. An activity of 1-6 X 10(5) ct/min/0-1 ml was obtained. The biological properties (inhibition of tumour growth and hepatosplenomegaly) of the labelled C. parvum were compared with those of commercially available vaccine, and were found to be similar. Labelled C. parvum was injected i.v., i.p., or s.c. into normal C57BL mice and the localization of activity determined at 4 h and 1,3,7 and 14 days after injection. After i.v. or i.p. injection, highest counts were recorded in the liver. Moderate activity was found in the spleen, lungs and small gut. After s.c. injection, the majority of radioactive label was detected at the site of injection and little found in other tissues. The distribution of injected C. parvum was also studied in mice bearing Lewis tumour, and was found to be similar to that in normal mice. Moderate amounts of labelled C. parvum were recovered from tumour. There appeared to be no relationship between the antitumour effect of C. parvum given by a particular route of injection and the concentration of C. parvum recovered from the tumour. Images Fig. 7 Fig. 8

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Section: Discussionsupporting

confidence: 88%

Radiolabelling of Corynebacterium parvum and its distribution in mice

Sadler¹,

Cramp²,

Castro³

1977

Br J Cancer

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“…There is experimental evidence that immunotherapy with i.v. Corynebacterium parvum causes a slight but significant increase in the survival of BALB/c mice injected intracerebrally with methylcholanthrene-induced sarcoma (Osborn et al, 1975). This finding may be relevant to the apparent protection of the CNS in our C +I group from clinically overt leukaemic infiltration.…”

Section: Resultsmentioning

confidence: 56%

“…Between October 1975 andJuly 1977, 32 adults wvith acute non-lymphoblastic leukaemia were entered into the trial. There were 17 males and 15 females, and their ages ranged from 18 to 65 (mean 48) years.…”

Section: Methodsmentioning

confidence: 99%

Immunotherapy using BCG during remission induction and as the sole form of maintenance in acute myeloid leukaemia

Summerfield¹,

Gibbs²,

Bellingham³

1979

Br J Cancer

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Summary.-Thirty-two adults with acute myeloid leukaemia (AML) were randomized to receive, from the time of diagnosis, either chemotherapy alone (C group) or chemotherapy plus Bacille Calmette-Guerin vaccine (BCG) (C + I group). After remission induction and consolidation, chemotherapy was stopped in both groups but BCG was continued in the C + I group.The overall survival of the C + I group was significantly increased (P < 0-05). There was no significant increase in the duration of first remission in the C + I group (0 05 0.1) but reduction in survival after CNS relapse was highly significant (P=0 001). These results suggest that administration of BCG from an early stage in the treatment of AML may protect the CNS against leukaemic infiltration and therefore serve as a simple, innocuous form of CNS prophylaxis.

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Experimental non-specific immunotherapy

Sadler¹,

Castro²

1978

Immunological Aspects of Cancer

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Effects of C. parvum on growth and induction of intracerebral tumours in mice

Cited by 5 publications

References 13 publications

Radiolabelling of Corynebacterium parvum and its distribution in mice

Radiolabelling of Corynebacterium parvum and its distribution in mice

Immunotherapy using BCG during remission induction and as the sole form of maintenance in acute myeloid leukaemia

Experimental non-specific immunotherapy

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