Effects of Bush Sophora Root polysaccharide and its sulfate on immuno-enhancing of the therapeutic DVH

Chen, Yun; Wu, Yi; Xian, Luanting; Song, Meiyun; Zeng, Ling; Xiong, Wen; Liu, Jiaguo; Sun, Wuping; Wang, Deyun; Hu, Yuanliang

doi:10.1016/j.ijbiomac.2015.06.029

Cited by 16 publications

(17 citation statements)

References 25 publications

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“… 2012 ; Chen et al. 2015b ). T cells secrete IL-2, IFN-γ and other cytokines to improve cell-mediated immunity (Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

“…PHA and LPS are a T-cell mitogen and a B-cell mitogen, respectively, and were able to stimulate T- and B-lymphocyte proliferation (Chen et al. 2015b ). In the present study, the T lymphocyte-stimulating ability of BLIN was the same as PHA and the B lymphocyte-stimulating ability of BLIN was significantly higher ( p < 0.05) than LPS.…”

Section: Discussionmentioning

confidence: 99%

“…The B lymphocyte proliferation rate was calculated based on the formula: Proliferation rate (%) = ( A test group − Ā control group )/ Ā control group × 100% (Chen et al. 2015b ).…”

Section: Methodsmentioning

confidence: 99%

“…After injecting DHAV-1 for 4, 8 and 54 h (Chen et al. 2015a , 2015b ), blood samples were randomly taken from five ducklings per group. The serum was separated and the IL-2, IFN-γ and total anti-DHAV-1 antibody levels were determined by an ELISA kit (Kamiya Biomedical Company, Seattle, WA) (Chen et al.…”

Section: Methodsmentioning

confidence: 99%

“…The serum was separated and the IL-2, IFN-γ and total anti-DHAV-1 antibody levels were determined by an ELISA kit (Kamiya Biomedical Company, Seattle, WA) (Chen et al. 2015b ). All these kits were specific for ducks.…”

Section: Methodsmentioning

confidence: 99%

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Anti-DHAV-1 reproduction and immuno-regulatory effects of a flavonoid prescription on duck virus hepatitis

Chen

Zeng

Yang

et al. 2017

Pharmaceutical Biology

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Context: The flavonoid prescription baicalin-linarin-icariin-notoginsenoside R1 (BLIN) has a curative effect on duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1). However, the mechanism of this curative effect is not understood.Objective: This study investigates the mechanism of the curative effect of BLIN on DVH caused by DHAV-1. We analyzed the anti-DHAV-1 reproduction mechanism and immuno-regulatory effect of BLIN.Materials and methods: The anti-DHAV-1 reproduction effects of BLIN at 20, 10, 5 and 2.5 μg/mL in vitro, as well as the influence of BLIN at 20 μg/mL on DHAV-1 adsorption, replication and release were tested using the qRT-PCR method. The promotion abilities of BLIN at 20, 10, 5 and 2.5 μg/mL on T- and B-lymphocyte proliferation were investigated by the MTT method. IL-2 and IFN-γ levels and total anti-DHAV-1 antibody secretion after treatment with DHAV-1 for 4, 8 and 54 h were determined by ELISA.Results: BLIN showed a dose-dependent DHAV-1 reproduction inhibitory effect. The inhibitory effect was highest at 20 μg/mL, where DHAV-1 adsorption and release were significantly lower. Meanwhile, BLIN at 5 μg/mL significantly increased T and B lymphocyte proliferation. BLIN stimulated total anti-DHAV-1 antibody secretion in ducklings at the dosage of 4 mg per duckling, but did not stimulate IL-2 and IFN-γ secretion significantly.Conclusions: BLIN inhibits DHAV-1 reproduction by suppressing its adsorption and release. Additionally, BLIN promoted the duckling antiviral response.

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“… 2012 ; Chen et al. 2015b ). T cells secrete IL-2, IFN-γ and other cytokines to improve cell-mediated immunity (Zhang et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The B lymphocyte proliferation rate was calculated based on the formula: Proliferation rate (%) = ( A test group − Ā control group )/ Ā control group × 100% (Chen et al. 2015b ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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