Effects of broad band electromagnetic fields on HSP70 expression and ischemia-reperfusion in rat hearts

Ronchi, Raffaella; Marano, Lidia; Braidotti, Paola; Bianciardi, Paola; Calamia, M.; Fiorentini, Cesare; Samaja, Michele

doi:10.1016/j.lfs.2003.12.033

Cited by 13 publications

(10 citation statements)

References 30 publications

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“…Much research has also been conducted at radiofrequencies. Ronchi et al [2004] found an increase in Hsp70 from broadband fields at 11.4 mT, but not at 36.1 mT, despite the fact that there were potential heating effects from the higher exposure (discussed earlier). No effect was seen on Hsp70 or Hsp27 in human leukocytes at 900 MHz for various SARs (0.4-3.6 W/kg) for short durations [20 min, 1 or 4 h; Lim et al, 2005], although Kwee et al [2001] did see significant increases in Hsp70 in AMA cells exposed to even lower SARs at 960 MHz (2.1 mW/kg for 20 min).…”

Section: Mechanisms: Heat Shock Proteinsmentioning

confidence: 78%

“…In a longer term study on broad band radiofrequency fields (0.2-200 MHz) at two different magnetic field intensities (11.4 and 36.1 mT) given to rats for short exposures (<10 min) daily over 3 weeks, Ronchi et al [2004] found changes in isolated heart function following ischemia-reperfusion in the lower field intensity exposure. These functional changes indicated that the magnetic field treatments decreased ischemic tolerance relative to controls.…”

Section: Magnetic Field Therapymentioning

confidence: 98%

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The influence of extremely low frequency magnetic fields on cytoprotection and repair

Robertson

Thomas

Bureau

et al. 2006

Bioelectromagnetics

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Ischemia-reperfusion injuries, such as those suffered from various types of cardiovascular disease, are major causes of death and disability. For relatively short periods of ischemia, much of the damage is potentially reversible and in fact, does not occur until the influx of oxygen during the reperfusion stage. Because of this, there is a window of opportunity to protect the ischemic tissue. Here, we review several mechanisms of protection, such as heat shock proteins, opioids, collateral blood flow, and nitric oxide induction, and the evidence indicating that magnetic fields may be used as a means of providing protection via each of these mechanisms. While there are few studies demonstrating direct protection with magnetic field therapies, there are a number of published reports indicating that electromagnetic fields may be able to influence some of the biochemical systems with protective applications.

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Section: Mechanisms: Heat Shock Proteinsmentioning

confidence: 78%

Section: Magnetic Field Therapymentioning

confidence: 98%

The influence of extremely low frequency magnetic fields on cytoprotection and repair

Robertson

Thomas

Bureau

et al. 2006

Bioelectromagnetics

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“…Repeated independent evaluations were averaged; statistical signiWcance calculation by two-tailed Student t test gave the P values reported between parenthesis ND Not done, NS not signiWcant (P > 0.05) Lennon et al 2004;Moran et al 2004), the underlying mechanism is not well understood (Melling et al 2004). The well known relevance of heat shock proteins in the prevention of cardiovascular diseases (Ji 2002) may involve inhibition of apoptosis (Siu et al 2004) and regulation of Ca2+ homeostasis (Liu et al 2006), but it is likely that the protection conferred by Hsp70 may not be suYcient to explain increased ischemia tolerance (Ronchi et al 2004) and may require additional activities, as for example those provided by HO-1 and Cox-2. The observed downregulation of other genes of the heat shock pathway, including Hsj2, Hsp60 and GRP75/PBP74, may be interpreted in terms of increased mitochondrial stress, but further studies are needed to address this issue.…”

Section: Discussionmentioning

confidence: 99%

Mild exercise training, cardioprotection and stress genes profile

et al. 2007

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To improve current knowledge of the molecular mechanisms underlying exercise-induced cardioprotection in a rat model of mild exercise training, Sprague-Dawley rats were trained to run on a treadmill up to 55% of their maximal oxygen uptake for 1 h/day, 3 days/week, 14 weeks, with age-matched sedentary controls (n = 20/group). Rats were sacriWced 48 h after the last training session. Despite lack of cardiac hypertrophy, training decreased blood hemoglobin (7.94 § 0.21 mM vs. 8.78 § 0.23 mM, mean § SE, P = 0.01) and increased both plasma malondialdehyde (0.139 § 0.005 mM vs. 0.085 § 0.009 mM, P = 0.05) and the activity of Mn-superoxide dismutase (11.6 § 0.6 vs. 16.5 § 1.6 mU/ g, P = 0.01), whereas total superoxide dismutase activity was unaVected. When subjected to 30-min ischemia followed by 90-min reperfusion, hearts from trained rats (n = 5) displayed reduced infarct size as compared to controls (37.26 § 0.92% vs. 49.09 § 2.11% of risk area, P = 0.04). The biochemical analyses in the myocardium, which included gene expression proWles, realtime PCR, Western blot and determination of enzymatic activity, showed training-induced upregulation of the following mRNAs and/or proteins: growtharrest and DNA-damage induced 153 (GADD153/ CHOP), heme-oxygenase-1 (HO-1), cyclooxygenase-2 (Cox-2), heat-shock protein 70/72 (HSP70/72), whereas heat-shock protein 60 (HSP60) and glucoseregulated protein 75 (GRP75) were decreased. As a whole, these data indicate that mild exercise training activates a second window of myocardial protection against ischemia/reperfusion by upregulating a number of protective genes, thereby warranting further investigation in man.

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“…On the other hand, the therapeutic effect of the ELF-EMF has been widely studied over the last few years, focusing on knowing the effect of these fields on genetic expression (Dicarlo et al, 1999;Ronchi et al, 2004). Also, Tokalov and Gutzeit (2004) evaluated the effect of 50 Hz MF on stress genes in the cellular line HL-60 on the expression of hsp27, hsp60, hsp70(A, B and C), hsc70, hsp75, hsp78 and hsp90 genes using RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

Effect of 60 Hz electromagnetic fields on the activity of hsp70 promoter: An in vitro study

Fuente

Alcocer‐González

Heredia‐Rojas

et al. 2009

Cell Biology International

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We have evaluated the effect of 60 Hz sinusoidal magnetic fields (MF) at 8 and 8 microT on expression of the luciferase gene contained in a gene construct labelled as Electromagnetic Field-plasmid (pEMF). The vector included the hsp70 promotor containing the 3 nCTCTn sequences previously described for the induction of hsp70 expression by magnetic fields, as well as the reporter of the luciferase gene. We also replicated the study of Lin et al. [Lin H, Blank M, Rossol-Haseroth K, Goodman R. Regulating genes with electromagnetic response elements. J Cell Biochem 2001;81(1):143-48]. The pEMF plasmid was transfected into HeLa and BMK16 cell lines that were later exposed to either MF or thermal shock (TS). An increased luciferase expression was found in both the cells exposed to MF and TS compared with their control groups (P < 0.05). Furthermore, the combined effect of MF and TS was also analyzed. A synergistic effect between two factors was observed for this co-exposure condition in terms of luciferase gene expression.

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Effects of broad band electromagnetic fields on HSP70 expression and ischemia-reperfusion in rat hearts

Cited by 13 publications

References 30 publications

The influence of extremely low frequency magnetic fields on cytoprotection and repair

The influence of extremely low frequency magnetic fields on cytoprotection and repair

Mild exercise training, cardioprotection and stress genes profile

Effect of 60 Hz electromagnetic fields on the activity of hsp70 promoter: An in vitro study

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