Effects of Botulinum Toxin A on Cytokine Synthesis in a Cell Culture Model of Cutaneous Scarring

Effects of botulinum toxin A on patient‐specific keloid fibroblasts in vitro

“…In addition, the increased metabolic requirements during wound healing demand neovascularization. As previously reported, we observed no effects of BoNT on microvascular endothelial cells . A recent animal study suggested that intradermal injection of BoNT could favor microcirculation by increased expression of VEGF .…”

“…As previously reported, we observed no effects of BoNT on microvascular endothelial cells. 10 A recent animal study suggested that intradermal injection of BoNT could favor microcirculation by increased expression of VEGF. 21 The detailed mechanism of this observation remains unknown.…”

“…8,9 In a first study, we reported on the effects of BoNT on microvascular endothelial cells and fibroblasts in vitro with respect to cell proliferation and cytokine synthesis. 10 These experiments were performed on cells derived from nonkeloid human skin. BoNT concentrations of 1.0 IU, 2.5 IU, 5.0 IU, and 10.0 IU were used.…”

Botulinum toxin and platelet rich plasma as innovative therapeutic modalities for keloids

“…When it is injected locally in keloids, it inhibits synaptic acetylcholine vesicle neuroexocytosis through proteolytic (d) regulation of the JNK signaling pathway. [22][23] However, Haubner et al, 24 demonstrated that neither cell proliferation, growth nor cytokines were affected by BTX-A incubation.…”

“…It was suggested that, BXT‐A suppresses fibroblast cell cycle and activity via several mechanisms such as: (a) inhibition of CTGF, TGF‐β1, VEGF, and MMP‐1 (b) upregulation of S100A, (c) suppression of myofibroblast differentiation, 21 (d) regulation of the JNK signaling pathway 22‐23 . However, Haubner et al, 24 demonstrated that neither cell proliferation, growth nor cytokines were affected by BTX‐A incubation.…”