Effects of benzo(a)pyrenf and tetrachlorodibenzo(p) dioxin on fetal dolphin kidney cells: Inhibition of proliferation and initiation of DNA damage

Carvan, Michael J.; Flood, Larry P.; Campbell, Barbra D.; Busbee, David L.

doi:10.1016/0045-6535(94)00395-b

Cited by 20 publications

(11 citation statements)

References 31 publications

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“…Our multidays experiments suggest a similar process occurs in marine mammals, both in somatic and reproductive tissues. This is in agreement with and builds upon previous studies showing that the enzyme CYP1A1 is inducible in cetacean fibroblasts (Godard et al, 2004) and that BP can inhibit mitosis and form DNA adducts in a dose-dependent and CYP1 induction-dependent manner in fetal dolphin kidney epithelial cells (Carvan et al, 1995). In addition, cytotoxicity observed in the testis was higher than that observed in somatic organs after 48 h (p < 0.01) and close to 100% after 72 h, indicating that further examination of the potential effects of PAH on NARW reproductive health is warranted.…”

supporting

confidence: 92%

Benzo[a]pyrene cytotoxicity in right whale (Eubalaena glacialis) skin, testis and lung cell lines

Godard

Wise

Kelly

et al. 2006

Marine Environmental Research

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supporting

confidence: 92%

Benzo[a]pyrene cytotoxicity in right whale (Eubalaena glacialis) skin, testis and lung cell lines

Godard

Wise

Kelly

et al. 2006

Marine Environmental Research

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“…In cultures of cetacean (dolphin) kidney epithelial cells PAH exposure caused DNA damage and inhibited cell growth and exposure to fuel oil caused apoptosis and altered transport and enzyme activities after high exposure levels (Carvan et al,1995;Pfeiffer et al,2000;Sweat et al,2001). However, there are no studies with endothelial cells cultured from cetacean tissues which describe the dose dependent responses to toxicant exposure.…”

Section: Introductionmentioning

confidence: 97%

Cytochrome P4501A is induced in endothelial cell lines from the kidney and lung of the bottlenose dolphin, Tursiops truncatus

et al. 2006

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Marine mammals respond to the presence of polycyclic and planar halogenated aromatic hydrocarbons (PAH or PHAH) with the induced expression in endothelium of cytochrome P4501A1, regulated through the aryl hydrocarbon receptor (AHR) transcription factor. Physiological responses in other animals, such as edema and inflammation indicate that the endothelium may be compromised by exposure to AHR agonists, which are ubiquitous in the marine environment. In other mammals and fish the cellular and molecular consequences of exposure to AHR agonists have been elucidated in cultured endothelial cells. We have cultured and characterized cetacean endothelial cells (EC) and used them in induction studies. Endothelial cells were cultured from the lung and kidney of the bottlenose dolphin Tursiops truncatus and exposed to the AHR agonists β-naphthoflavone (βNF) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). βNF (1-3 µM) induced significant increases in CYP1A1(O-deethylation of 7-ethoxyresorufin to resorufin;EROD) activity to 3.6 and 0.92 pmol/mg/min in lung and kidney EC, respectively. TCDD was more potent than βNF, and more efficacious, with maximum induction of CYP1A1activity of 10.1 and 15.2 pmol/mg/min in lung and kidney EC at 3-10 nM TCDD. The differential response indicates that the lung and kidney endothelial cells in culture retain the ability to respond in a selective manner to specific stimuli. Both the molecular mechanisms of induction and the physiological consequences, especially in the vasculature, of toxicant exposure can be studied in this system.

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“…In other cetacean species, a number of cell lines have been developed in order to assess viral impact (Cecil and Nigrelli, 1970, Nielsen et al, 1989, Kadoi et al, 1992 and the effects of contaminants (Wang and Pfeiffer, 2001, Fossi et al, 2006, Li Chen et al, 2009, Carvan et al, 1995, Spinsanti et al, 2008, Wise et al, 2010, Gauthier et al, 1999, Pine et al, 2007. Toxic effects in humpback whales are, however, still poorly understood and there are, to date, no established methods to study toxicological sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HuWa1 exposed to a natural, p,p'-DDE-containing, chemical mixture extracted from whale blubber showed distinctively higher sensitivity than to p,p'-DDE alone. Thus, we provide the first cytotoxicological data for humpback whales and with establishment of the HuWa cell lines, a unique in vitro model for the study of the whales' sensitivity and cellular response to chemicals and other environmental stressors. Establishment

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Effects of benzo(a)pyrenf and tetrachlorodibenzo(p) dioxin on fetal dolphin kidney cells: Inhibition of proliferation and initiation of DNA damage

Cited by 20 publications

References 31 publications

Benzo[a]pyrene cytotoxicity in right whale (Eubalaena glacialis) skin, testis and lung cell lines

Benzo[a]pyrene cytotoxicity in right whale (Eubalaena glacialis) skin, testis and lung cell lines

Cytochrome P4501A is induced in endothelial cell lines from the kidney and lung of the bottlenose dolphin, Tursiops truncatus

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

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