Effects of Bacillus amyloliquefaciens LFB112 on Growth Performance, Carcass Traits, Immune, and Serum Biochemical Response in Broiler Chickens

Abstract

This study aimed to investigate the effects of Bacillus amyloliquefaciens LFB112 on the growth performance, carcass traits, immune response, and serum biochemical parameters of broiler chickens. A total of 396 1 day old, mixed-sex commercial Ross 308 broilers with similar body weights were allotted into six treatment groups. The assigned groups were the CON group (basal diet with no supplement), AB (antibiotics) group (basal diet + 150 mg of aureomycin/kg), C+M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 powder with vegetative cells + metabolites), C group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 vegetative cell powder with removed metabolites), M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 metabolite powder with removed vegetative cells), and CICC group (basal diet + 5 × 108 CFU/kg Bacillus subtilis CICC 20179). Results indicated that chickens in the C+M, C, and M groups had higher body weight (BW) and average daily gain (ADG) (p < 0.05) and lower feed conversion ratio (FCR) (p = 0.02) compared to the CON group. The C+M group showed the lowest abdominal fat rate compared to those in the CON, AB, and CICC groups (p < 0.05). Compared to the CON group, serum IgA and IgG levels in the C+M, C, and M groups significantly increased while declining in the AB group (p < 0.05). B. amyloliquefaciens LFB112 supplementation significantly reduced the serum triglyceride, cholesterol, urea, and creatinine levels, while increasing the serum glucose and total protein (p < 0.05). In conclusion, B. amyloliquefaciens LFB112 significantly improved the growth performance, carcass traits, immunity, and blood chemical indices of broiler chickens and may be used as an efficient broiler feed supplement.

Enhancing egg production and quality by the supplementation of probiotic strains (Clostridium and Brevibacillus) via improved amino acid digestibility, intestinal health, immune response, and antioxidant activity

“…This lends evidence that the newly designed strains of C. butyricum and Brevibacillus are suitable for peak-laying hens. They could also be used as a safe feed additive in the poultry industry as reported previously with other probiotic strains ( Wei et al, 2020 ; Yang et al, 2020 ; Ahmat et al, 2021 ; César et al, 2022 ). There is a dearth of evidence on the influence of Brevibacillus sp.…”

“…The immunity index of animals is often measured based on the serum concentrations of immunoglobulins (IgM, IgA, and IgG) due to their key role in immune regulation and disease resistance ( Mountzouris et al, 2010 ). Previous reports demonstrated that B. amyloliquefaciens significantly increased serum concentrations of IgG and IgA ( Ahmat et al, 2021 ) and the probiotic complex ( Deng et al, 2021 ), and B. subtilis ( Qiu et al, 2021 ) enhanced the serum immunoglobulins of IgG, IgA, and IgM in broiler birds. There are pieces of evidence that B. amyloliquefaciens increased IgG and IgA levels ( Zhou et al, 2020 ) and CB enhanced IgA, IgY, and IgM ( Zhan et al, 2019 ) in laying hens.…”

Bacillus Probiotics as Alternatives to In-feed Antibiotics and Its Influence on Growth, Serum Chemistry, Antioxidant Status, Intestinal Histomorphology, and Lesion Scores in Disease-Challenged Broiler Chickens

“…Increased abdominal fat content connotes inefficient use of energy in the feed. It is evident from the findings of Ahmat et al ( 73 ) that abdominal fat weights were significantly lower in birds offered diets supplemented with B. amyloliquefaciens (5 × 10 5 cfu/g) and antibiotic (150 mg of aureomycin/kg) compared to control birds. This finding is in harmony with other authors ( 62 , 78 , 79 ), who found significantly lower abdominal fat weights in chickens fed Bacillus probiotics feed additives.…”

“…Recently, Ahmat et al ( 73 ) assessed the impact of B. amyloliquefaciens LFB112 and B. subtilis CICC 20179 on performance of broiler chickens and observed that broilers fed B. amyloliquefaciens at 5 × 10 5 cfu/g had better BWG, FCR, carcass yield and cut-part weights (thigh and breast muscle) than broilers treated with B. subtilis (5 × 10 5 cfu/g) and antibiotic (150 mg of aureomycin/kg). Based on their findings, supplementation of B. subtilis and aureomycin to the level of 5 × 10 5 cfu/g and 150 mg/kg feed, respectively in broiler chickens did not enhance growth performance, suggesting that B. subtilis and aureomycin may not have reached the threshold required to improve growth and productivity in broilers.…”

“…Serum immunoglobulins (Ig), especially those generated from the b cells are used as indicators of humoral immunological state in poultry because of their role in immune regulation and resistance to several diseases ( 97 , 98 ). Serum Ig levels in broilers were affected by Bacillus probiotics as Ahmat et al ( 73 ) observed increased serum IgA and IgG in healthy broilers fed aureomycin at 150 mg/kg, and Bacillus probiotic ( B. amyloliquefaciens and B. subtilis ) and their metabolites at the level of 5.0 × 10 5 cfu/g in their diet. These results indicate that Bacillus probiotics and their metabolites that increased serum immunoglobulin concentrations will have more beneficial impacts on growth performance and broiler chickens' ability to combat diseases.…”