Effects of auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis induction from shoot apices of pea

Loiseau, Jacques; Marche, C.; Deunff, Yvon Le

doi:10.1007/bf00045091

Cited by 52 publications

(26 citation statements)

References 31 publications

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“…1) gave the highest mean number of 15.2 that was significantly higher than fructose induced on the same media (9.9 shoots), higher than lactose induced cultures on D2B2 (Table 2). Similar result of different shoot production response through somatic embryogenesis due to different types of sugar was reported in peas (Loiseau et al 1995), in pine (Nagmani et al 1993) and in alfalfa (Strickland et al 1987). 2,4-D concentration in the medium also played important role in the number of shoot production, lower concentration such as 2 mg/l responded significantly better than 4 mg/l in both 7.5 and 9 month-old cultures.…”

Section: Resultssupporting

confidence: 77%

Effect of Sugar Types, Culture Age, Concentrations of 2,4?D and Sucrose on Somatic Embryogenesis of Cymbopogon schoenanthus subsp. proximus

Salam

Chowdhury

Bakry

2015

Plant Tissue Cult. & Biotech.

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The effect of sugar types, age of culture, and concentrations of 2,4-D and sucrose on somatic embryogenesis was investigated in Cymbopogon schoenanthus subsp. proximus. Six different sugar types: sucrose, fructose, maltose, glucose, galactose and lactose at 3% concentration were used for each of three 2,4-D concentration treatments (1.0, 2.0, 4.0 mg/l). ANOVA showed a significant difference at the 1% level for culture age, sugar types and interactions between 2,4-D and sugar types when mean embryo numbers of individual sugars across the four culture ages are compared. Maltose gave highest mean value, followed by sucrose and the lowest mean number was recorded with galactose. Cultures induced by galactose although low in embryo induction gave the highest mean number of shoots across the different media and culture age. Effect of different concentrations of sucrose and BA on production of number of immature somatic embryos, mature germinating somatic embryos and shoots was examined by culturing 2.5 month-old seed-derived callus onto MS medium containing 4 different sucrose concentrations (1.0, 2.0, 3.0, 4.0 %) in combination with different BA concentrations (0.0, 0.025, 0.05, 0.1 mg/l). ANOVA showed significant differences at 1% level for culture age, and interactions between culture age and sucrose concentrations. Mean number of shoots on 1% sucrose for 7 months old culture was 9.4 significantly higher than number produced on all of sucrose concentrations. The efficient regeneration system will be useful for the future production of high yielding genotypes and for the conservation of the species germplasm.

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Section: Resultssupporting

confidence: 77%

Effect of Sugar Types, Culture Age, Concentrations of 2,4?D and Sucrose on Somatic Embryogenesis of Cymbopogon schoenanthus subsp. proximus

Salam

Chowdhury

Bakry

2015

Plant Tissue Cult. & Biotech.

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“…In Agapanthus africanus, however, somatic embryogenesis was not induced by 2, 4-D but by picloram. The effectiveness of picloram for inducing somatic embryogenesis has also been reported in some other monocotyledonous plants [10,11] as well as some dicots such as pea [12] and chickpea [13]. Because of the high ability in regeneration and multiplication, the plant regeneration systems of agapanthus established in the present study will be efficiently used for rapid clonal propagation if the micropropagated plants have no aberrant somaclonal variations.…”

Section: Discussionsupporting

confidence: 67%

Organogennsis and Somatic Embryogenesis from Young Flower Buds of Agapanthus africanus Hoffmanns.

Supaibulwatana

Mii

1997

Plant Biotechnology

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Adventitious shoots and roots were directly formed from young flower bud explants of Agapanthus africanus on Murashige and Skoog (MS) medium containing 30 g¥L-1 sucrose, 2 g¥L-1 gellan gum, and kinetin or thidiazuron at 1 mg¥L-1 in combination with 1 mg¥L-1 NAA. Friable calli with a high rate of plant regeneration were also induced by culturing the explants on medium containing NAA, picloram or 2, 4-D at concentrations varying from 1 to 10 mg¥L-1, especially at low concentrations of picloram or 2, 4-D. Shoots were readily induced from these calli when transferred onto plant growth regulator-free or 1 mg¥L-1 zeatincontaining MS medium. Nodular compact calli were also formed with friable calli on medium supplemented with 2, 4-D or picloram. The compact calli induced with 1 mg¥L-1 picloram developed into somatic embryos.In both shoot and embryo regeneration systems, complete plantlets were obtained when they were transferred onto a medium containing no plant growth regulator.

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“…Conversely, no studies have described the use of the auxin 4-CPA to induce embryogenesis in the papaya. However, 4-CPA has been successfully used in other species such as Pisum sativum (Loiseau et al 1995), Rosa sp. Auxins are involved in regulating the cell cycle and promoting cell differentiation and play important roles in somatic embryogenesis in several species (Jiménez 2005, Kurczynska et al 2007, Capelo et al 2011, Rocha et al 2012).…”

Section: Induction Of Somatic Embryogenesismentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heartshape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L -1 ) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus -1 ) and the lowest percentage of early germination (4%).

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Effects of auxins, cytokinins, carbohydrates and amino acids on somatic embryogenesis induction from shoot apices of pea

Cited by 52 publications

References 31 publications

Effect of Sugar Types, Culture Age, Concentrations of 2,4?D and Sucrose on Somatic Embryogenesis of Cymbopogon schoenanthus subsp. proximus

Effect of Sugar Types, Culture Age, Concentrations of 2,4?D and Sucrose on Somatic Embryogenesis of Cymbopogon schoenanthus subsp. proximus

Organogennsis and Somatic Embryogenesis from Young Flower Buds of Agapanthus africanus Hoffmanns.

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

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