Organogennsis and Somatic Embryogenesis from Young Flower Buds of Agapanthus africanus Hoffmanns.

Abstract

Adventitious shoots and roots were directly formed from young flower bud explants of Agapanthus africanus on Murashige and Skoog (MS) medium containing 30 g¥L-1 sucrose, 2 g¥L-1 gellan gum, and kinetin or thidiazuron at 1 mg¥L-1 in combination with 1 mg¥L-1 NAA. Friable calli with a high rate of plant regeneration were also induced by culturing the explants on medium containing NAA, picloram or 2, 4-D at concentrations varying from 1 to 10 mg¥L-1, especially at low concentrations of picloram or 2, 4-D. Shoots were readily induced from these calli when transferred onto plant growth regulator-free or 1 mg¥L-1 zeatincontaining MS medium. Nodular compact calli were also formed with friable calli on medium supplemented with 2, 4-D or picloram. The compact calli induced with 1 mg¥L-1 picloram developed into somatic embryos.In both shoot and embryo regeneration systems, complete plantlets were obtained when they were transferred onto a medium containing no plant growth regulator.

Induction of Meristematic Nodular Calli from Various Explants of Lilium spp. and Long Term Stability in Plant Regeneration Ability and Ploidy Level of the Calli.

“…The present study demonstrates the advantage of picloram over other auxins for callus induction in various species of lily. There also is the report of picloram-induced embryogenic calli in a related species of Liliaceae [15]. A relatively low concentration (1mg/l) of picloram was effective for the induction of NOD under light condition and for the maintenance of the calli without changing the characteristics during the subcultures.…”

Clonal propagation of Lomandra longifolia by somatic embryogenesis

“…Notably, somatic embryogenesis has been successfully induced in grass-like plants such as Cyperaceae (sedges), the north American wetland species Scirpus robustus (Wang et al, 2004) and recently in Lepidosperma drummondii, an important plant for postmining restoration in Western Australia (Panaia et al, 2011). In the Liliaceous monocots, plant regeneration from organogenic or embryogenic callus or suspension cultures was demonstrated for several species, including Agapanthus africanus (Supaibulwatana and Mii, 1997), Allium spp. (Kim and Soh, 1996), Asparagus officinalis (Kunitake and Mii, 1998), Hemerocallis spp.…”