Effects of atrazine on cytochrome P450 enzymes of zebrafish (Danio rerio)

Dong, Xiaohui; Zhu, Lusheng; Wang, Jinhua; Wang, Jun; Xie, Hui; Hou, Xinxin; Wen-tao, Jia

doi:10.1016/j.chemosphere.2009.06.052

Cited by 107 publications

(41 citation statements)

References 65 publications

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“…It has been reported that terbutryn is able to induce EROD activity in rainbow trout (Tarja et al, 2003). Dong et al (2009) reported that P450 content in zebrafish (Danio rerio) (both male and female) was induced by atrazine, even at a dose of 0.01 mg/L. Exposure to terbutryn and simazine did not influence CYP concentration or EROD, GST, or GSH activity, suggesting that a role for CYP450 1A in the metabolism of terbutryn and simazine in common carp can be ruled out.…”

Section: Triazinesmentioning

confidence: 99%

The Effects of Pyrethroid and Triazine Pesticides on Fish Physiology

Velíšek¹,

Stará²,

Svobodová³

2011

Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment

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Section: Triazinesmentioning

confidence: 99%

The Effects of Pyrethroid and Triazine Pesticides on Fish Physiology

Velíšek¹,

Stará²,

Svobodová³

2011

Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment

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“…It is worthy of note that atrazine can affect the liver antioxidant status and thus, retinoid isomerization (Singh et al 2011). Induction of NADPH cytochrome P450 reductase has been observed in adult zebra fish exposed to atrazine (Dong et al 2009) and in newt exposed to MCPA (4-chloro-2-methylphenoxy acetic acid) (Santagostino et al 1989). In the case of newt, low doses of MCPA increased glutathion-S-transferases, enzymes both implicated in the metabolism and the isomerization of RA.…”

Section: Discussionmentioning

confidence: 97%

Oxidation of retinoic acids in hepatic microsomes of wild bullfrogs Lithobates catesbeianus environmentally-exposed to a gradient of agricultural contamination

et al. 2012

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Agricultural contaminants are suspected of contributing to the increased incidence of deformities and the decline of amphibians populations worldwide. Many authors have further suggested that a retinoid effect could be implicated in teratogenic mechanisms since the reported deformities resemble those caused by abnormal levels of retinoic acid (RA). We previously reported altered retinoid concentrations in male bullfrogs from the Yamaska River basin (Québec, Canada) associated with moderate-to-high agricultural activity, and the findings were consistent with a possible effect on hepatic RA oxidation. An in vitro assay was therefore optimized and hepatic microsomal RA oxidation in bullfrogs was found to be quite different from that of other vertebrates. With either all-transRA (atRA) or 13cisRA as the substrate, the major metabolite generated was at4-oxo-RA. The reaction with 13cisRA as substrate, markedly greater compared with atRA, was enhanced in the presence of a reducing agent and inhibited by cytochrome P450 inhibitors in a dose-dependent manner. Hepatic RA oxidation in male bullfrogs showed significant differences between sites with no clear relationship to a gradient of agricultural activity or 13cis-4-oxo-RA quantified in plasma. In contrast, the in vitro RA oxidation in females increased with the levels of contamination and coincided in vivo with higher plasma 13cis-4-oxo-RA concentration. The levels of circulating 4-oxo-derivatives could be influenced by hepatic RA oxidative metabolism as well as isomerization conditions or RA precursor levels.

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“…The zebrafish (D. rerio) is a small equatorial freshwater fish that has a great tolerance for a wide range of breeding circumstances has been employed as an experimental species in the intensive studies of many other scientists, from the 1980s. Particularly in recent years, numerous studies have shown the identified benefits of hiring of zebrafish in environmental toxicological studies [3]. The use of herbicides to control weeds has been admitted, and its frequent applications as a part of farming practices worldwide.…”

Section: Introductionmentioning

confidence: 99%

“…Sublethal impacts with biochemical changes of fish tissues may occur with long term exposure to levels of lowest from 2 mg L −1 of atrazine [4]. Several writers have mentioned the effect of atrazine on the physiology, metabolism, growth and reproduction of aquatic organisms, special on fishes [8] and also can cause impaired embryonic growth [3]. Hence, it is necessary to identify certain parameters used in the assessment of environmental hazards of pesticides, as the degree of accumulation and stress biomarkers of atrazine in aquatic objects.…”

Section: Introductionmentioning

confidence: 99%

Bioconcentration and Antioxidant Status Responses in Zebrafish (Danio Rerio) Under Atrazine Exposure

Ghazie¹,

Sawafi²,

Yan³

2013

IJCEA

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Abstract-The present study was planned designed to investigate the chronic impacts of herbicide atrazine exposure on stress biomarkers acetylcholinesterase activity (AChE) and oxidative stress responses in brain of zebrafish (Danio rerio), and determine the bioconcentration of atrazine in whole body of fish. Chronic exposure to atrazine unveiled a markedly discourage in the activity of AChE. However, significant increase in the activities of catalase (CAT) and superoxide dismutase (SOD) and both influenced by atraizine, and CAT was over sensitivity to atrazine compared with SOD. The highest bioconcentration factor (BCF) of atrazine in the fish treated was (12.8543×10 4 and 13.5891×10 4 ) after 24h exposure to (0.957 and 1.913 mg L -1 ) and 13.238×10 4 , after 25 d exposure to 0.638 mg L −1 of atrazine respectively. This study showed that the zebrafish have ability to bioaccumulate of the herbicide atrazine rapidly. atrazine accumulation in zebrafish has a private importance due to the implication of atrazine in chronically health problems, as well as the information contained in our study is useful for understand the mechanism of atrazine induced oxidative stress in fish.

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Effects of atrazine on cytochrome P450 enzymes of zebrafish (Danio rerio)

Cited by 107 publications

References 65 publications

The Effects of Pyrethroid and Triazine Pesticides on Fish Physiology

The Effects of Pyrethroid and Triazine Pesticides on Fish Physiology

Oxidation of retinoic acids in hepatic microsomes of wild bullfrogs Lithobates catesbeianus environmentally-exposed to a gradient of agricultural contamination

Bioconcentration and Antioxidant Status Responses in Zebrafish (Danio Rerio) Under Atrazine Exposure

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