Effects of atrazine and chlorpyrifos on activity and transcription of glutathione S-transferase in common carp (Cyprinus carpio L.)

Xing, Houjuan; Wang, Xu; Sun, Gang; Gao, Xuejiao; Xu, Shiwen; Wang, Xiaolong

doi:10.1016/j.etap.2011.12.014

Cited by 89 publications

(28 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, results of qPCR reveal that CPF exposure downregulates CYP 3A transcription in goldfish liver, indicating that CPF is not the inducer of CYP 3A in goldfish and goldfish CYP 3A may be not involved in the CPF bioactivation. The results of acute toxicity test in this study reveal that 96-h LC 50 of CPF on goldfish was 153 lg L 21 , indicating that CPF is highly toxic to goldfish, which is in consistent with previous reports in common carp (Xing et al, 2012b), tilapia (Chandrasekara and Pathiratne, 2007), and zebrafish (Levin et al, 2004). However, the 96-h LC 50 for goldfish obtained in this study was lower than those obtained for the common carp (582 lg L 21 ) (Xing et al, 2012b), tilapia larvae (1.57 mg L 21 ) (G€ ul, 2005), and zebra fish (680 lg L 21 ) (Ji et al, 2010), whereas it was higher than that in Chirostoma jordani (0.17 lg L 21 ) (Dzul-Caamal et al, 2012).…”

Section: Discussionsupporting

confidence: 93%

Effects of chlorpyrifos on the transcription of CYP3A cDNA, activity of acetylcholinesterase, and oxidative stress response of goldfish (Carassius auratus)

Liu

Niu

et al. 2013

Environmental Toxicology

View full text Add to dashboard Cite

Chlorpyrifos (CPF) is the widely used organophosphate pesticide in agriculture throughout the world. It has been found that CPF is relatively safe to human but highly toxic to fish. In this study, acute toxicity of CPF on goldfish was determined and then the transcription of goldfish cytochrome P450 (CYP) 3A was evaluated after 96 h of CPF exposure at concentrations of 15.3 [1/10 50% lethal concentration (LC50 )] or 51 μg L(-1) (1/3 LC50 ) of CPF. Meanwhile, the enzymatic activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), and catalase (CAT), total antioxidant activity (T-AOC), and the contents of malondialdehyde (MDA) in the liver or brain of goldfish were also determined. The results of acute toxicity testing showed that the 96-h LC50 of CPF to the goldfish was 153 μg L(-1) . Moreover, a length sequence of 1243 bp CYP3A cDNA encoding for 413 amino acids from goldfish liver was cloned. Polymerase chain reaction results reveal that CPF exposure downregulates CYP 3A transcription in goldfish liver, suggesting that goldfish CYP 3A may be not involved in CPF bioactivation. Finally, the results of biochemical assays indicate that 96 h of CPF exposure remarkably inhibits AChE activity in fish liver or brain, alters hepatic antioxidant enzyme activities, decreases brain T-AOC, and causes lipid peroxidation in fish liver. These results suggest that oxidative stress might be involved in CPF toxicity on goldfish.

show abstract

Section: Discussionsupporting

confidence: 93%

Effects of chlorpyrifos on the transcription of CYP3A cDNA, activity of acetylcholinesterase, and oxidative stress response of goldfish (Carassius auratus)

Liu

Niu

et al. 2013

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…Based on 96 h LC 50 , it has been observed that Cirrhinus mrigala is more sensitive to chlorpyrifos than Oncorhynchus mykiss (24 [17], which rated chlorpyrifos as highly toxic to Cirrhinus mrigala as the value determined was 0.44 mg L −1 . This value is slightly lower than LC 50 96 h of chlorpyrifos in Channa punctatus reported by [7].…”

Section: Discussionmentioning

confidence: 99%

Genotoxic Effects of Chlorpyrifos in Freshwater Fish Cirrhinus mrigala Using Micronucleus Assay

Bhatnagar

Yadav

Cheema

2016

Advances in Biology

View full text Add to dashboard Cite

The genotoxicity of pesticides is an issue of worldwide concern and chlorpyrifos is one of the largest selling organophosphate agrochemicals that has been widely detected in surface waters of India. The studies on long term genotoxic biomarkers are limited; therefore, present study was carried out to analyze the incidence of nuclear anomalies in the blood cells of fresh water fish Cirrhinus mrigala using micronucleus (MN) assay as a potential tool for assessment of genotoxicity. Acute toxicity of chlorpyrifos was evaluated by exposing fingerlings to different doses of chlorpyrifos (1/20, 1/10, and 1/5 of LC 50 ) and LC 50 was calculated as 0.44 mg L −1 using probit analysis. Blood samples were taken on days 2, 4, 8, 12, 21, 28, and 35. In general, significant effects for both concentration and duration of exposure were observed in treated fish. It was found that MN induction was highest on day 14 at 0.08 mg L −1 concentration of chlorpyrifos. It was concluded that chlorpyrifos is genotoxic pesticide causing nuclear anomalies in Cirrhinus mrigala.

show abstract

“…The 96-h LC 50 values of CPF for juvenile and adult nile tilapia (Tilapia niloticus), common carp (Cyprinus carpio), mosquito fish (Gambusia affinis), and guppy (Poecilia reticulata) were 98.7 and 154.0 (Oruç 2010), 580 (Xing et al 2012), 297 (Kavitha and Rao 2008), and 175.8 lg/L (Sharbidre et al 2011), respectively.…”

Section: Discussionmentioning

confidence: 99%

Effect of Chlorpyrifos Ethyl on Acetylcholinesterase Activity in Climbing Perch (Anabas testudineus, Bloch, 1972)

Tâm

Berg

Tuyen

et al. 2015

Arch Environ Contam Toxicol

View full text Add to dashboard Cite

The high use of pesticides in intensive rice farming in the Mekong Delta constitutes a potential hazard to the environment and to people's health. Chlorpyrifos ethyl (CPF) is a commonly used organophosphate (OP) insecticide, but information about its potential negative impacts on the aquatic environment in the Mekong Delta is scarce. Both acute and subacute toxicity tests were performed in a static nonrenewable system to investigate the effects of CPF on brain acetylcholinesterase (AChE) activity in native climbing perch fingerlings (Anabas testudineus, Bloch, 1972). Environmental parameters, such as dissolved oxygen, water temperature, and pH, were similar to field conditions in the Mekong Delta. In a 96-h lethal concentration (LC50) test, fingerlings of climbing perch were randomly exposed to five levels of CPF ranging from 0.8 to 4.5 ppm. Five sublethal levels of CPF (1, 5, 10, 15, and 20 % of the 96-h LC50 value) were tested to assess the sensitivity and recovery of the brain AChE activity in climbing perch fingerlings exposed to CPF. The results showed that CPF were moderately toxic to climbing perch with a 96-h median LC50 of 1.73 ppm. CPF also caused long-term AChE inhibition with 70 % inhibition remaining after 96 h for the four highest test concentrations. The recovery of brain AChE activity in fish placed in CPF-free water was very slow, and after 7 days the brain AChE activity was still significant lower in fish from the four highest concentrations compared with the control. The results from this study indicate that OP insecticides, such as CPF, can have long-lasting sublethal effects on aquatic species in the Mekong Delta.

show abstract

Effects of atrazine and chlorpyrifos on activity and transcription of glutathione S-transferase in common carp (Cyprinus carpio L.)

Cited by 89 publications

References 52 publications

Effects of chlorpyrifos on the transcription of CYP3A cDNA, activity of acetylcholinesterase, and oxidative stress response of goldfish (Carassius auratus)

Effects of chlorpyrifos on the transcription of CYP3A cDNA, activity of acetylcholinesterase, and oxidative stress response of goldfish (Carassius auratus)

Genotoxic Effects of Chlorpyrifos in Freshwater Fish Cirrhinus mrigala Using Micronucleus Assay

Effect of Chlorpyrifos Ethyl on Acetylcholinesterase Activity in Climbing Perch (Anabas testudineus, Bloch, 1972)

Contact Info

Product

Resources

About