Effects of Atherosclerosis on Mean and Daily Variation of Arterial Pressure in Conscious WHHL Rabbits.

Katsuda, Shin-ichiro; Hara, Hiroshi; Shiomi, Masashi; Watanabe, Yoshio

doi:10.1292/jvms.54.669

Cited by 2 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). This is in agreement with a previous report on WHHL rabbit hypertension (19). When rabbits were placed on total heart bypass, WHHL and NZ rabbits exhibited similar pressure values.…”

Section: Discussionsupporting

confidence: 93%

Reduced lung endothelial angiotensin-converting enzyme activity in Watanabe hyperlipidemic rabbits in vivo

Orfanos

Parkerson²,

Chen

et al. 2000

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

We investigated pulmonary endothelial function in vivo in 12- to 18-mo-old male Watanabe heritable hyperlipidemic (WHHL; n = 7) and age- and sex-matched New Zealand White (n = 8) rabbits. The animals were anesthetized and artificially ventilated, and the chest was opened and put in total heart bypass. The single-pass transpulmonary utilizations of the angiotensin-converting enzyme (ACE) substrate [(3)H]benzoyl-Phe-Ala-Pro (BPAP) and the 5'-nucleotidase (NCT) substrate [(14)C]AMP were estimated, and the first-order reaction parameter A(max)/K(m), where A(max) is the product of enzyme mass and the catalytic rate constant and K(m) is the Michaelis-Menten constant, was calculated. BPAP transpulmonary utilization and A(max)/K(m) were reduced in WHHL (1.69 +/- 0.16 vs. 2.9 +/- 0.44 and 599 +/- 69 vs. 987 +/- 153 ml/min in WHHL and control rabbits, respectively; P < 0.05 for both). No differences were observed in the AMP parameters. BPAP K(m) and A(max) values were estimated separately under mixed-order reaction conditions. No differences in K(m) values were found (9.79 +/- 1 vs. 9.9 +/- 1.31microM), whereas WHHL rabbit A(max) was significantly decreased (5.29 +/- 0.88 vs. 7. 93 +/- 0.8 micromol/min in WHHL and control rabbits, respectively; P < 0.05). We conclude that the observed pulmonary endothelial ACE activity reduction in WHHL rabbits appears related to a decrease in enzyme mass rather than to alterations in enzyme affinity.

show abstract

“…1). This is in agreement with a previous report on WHHL rabbit hypertension (19). When rabbits were placed on total heart bypass, WHHL and NZ rabbits exhibited similar pressure values.…”

Section: Discussionsupporting

confidence: 93%