Effects of As2O3 on DNA methylation, genomic instability, and LTR retrotransposon polymorphism in Zea mays

Erturk, Fılız Aygun; Aydın, Murat; Sığmaz, Burcu; Taspinar, Mahmut Sinan; Arslan, Esra; Ağar, Güleray; Yağci, Semra

doi:10.1007/s11356-015-5426-2

Cited by 20 publications

(8 citation statements)

References 45 publications

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“…In this experiment, DNA hypermethylation was observed at higher Al concentrations, whereas hypomethylation was detected at lower Al concentrations. Similar and supporting results have been reported by several researchers in maize under zinc stress (Erturk et al, 2015a), chromium nitrate in maize (Erturk et al, 2014a), arsenic trioxide in Zea mays (Erturk et al, 2015b), and lead sulfate solution in Zea mays (Erturk et al, 2014b). Excessive accumulation of Al can reduce the activity of methyl transferase and cause hypomethylation of certain specific gene regions.…”

Section: Discussionsupporting

confidence: 89%

Estimation of genomic instability and DNA methylation due to aluminum (Al) stress in wheat ( Triticum aestivum L.) using iPBS and CRED-iPBS analyses

Türkoğlu¹,

Özkan²,

Nalci³

et al. 2019

Turk J Bot

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Aluminum (Al) toxicity is a serious factor restricting crop productivity in acid soil, and Al is the major cause of phytotoxicity. However, the role of Al toxicity in interprimer binding site (iPBS) polymorphism, genomic instability, and DNA methylation has not been fully investigated. In the current study, the effects of different Al concentrations on iPBS polymorphism, genomic instability, and DNA methylation were investigated in seedlings of three wheat cultivars: Haymana 79, Kılçıksız, and Bezostaja 1. A higher aluminum concentration increased the polymorphism rate of the iPBS profile, but decreased genomic template stability in all cultivars. A higher Al concentration was found to cause DNA methylation. Furthermore, the coupled restriction enzyme digestion-iPBS technique was used to detect DNA cytosine methylation level, which could help in understanding the epigenetic mechanism. The occurrence of hypermethylation and hypomethylation was observed with respect to Al stress treatment, and Al was found to cause DNA methylation. Polymorphism in the CRED-iPBS profile and DNA methylation can be correlated to evaluate epigenetic changes under stress.

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Section: Discussionsupporting

confidence: 89%

Estimation of genomic instability and DNA methylation due to aluminum (Al) stress in wheat ( Triticum aestivum L.) using iPBS and CRED-iPBS analyses

Türkoğlu¹,

Özkan²,

Nalci³

et al. 2019

Turk J Bot

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“…Similar results for heavy metals were published by Aina et al [20]. The first paper focused on the effect of As on DNA methylation in plants was published by Erturk et al [21]. Their results showed DNA hypermethylation of some genes in germinating maize seeds exposed to low As levels.…”

Section: Discussionsupporting

confidence: 78%

Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

et al. 2020

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Background: Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity. Results: The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg − 1 for 122 days, decreased with age. Content of As was higher in young than old fronds for variants with 100 mg As kg − 1 (2800 and 2000 mg As kg − 1 dry matter, respectively). The highest As content was determined in old fronds of Pc-Al grown in pots with 250 mg As kg − 1. The increase with age was confirmed for determined nutrients-Cu, Mg, Mn, S and Zn. A significant elevation of all analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25 to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn. Conclusions: The results of this paper point to changes in the metabolism of the hyperaccumulator plant Pc-Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator plant Pc-Al, especially in old fronds.

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“…In this respect, under salinity stress conditions the tomato plants exhibited significant changes in cytosine methylation status that can be referred to as hyper methylation. Likewise, several studies have demonstrated abiotic stresses, such as chromium nitrate, zinc, arsenic, and lead sulfate stress/toxicity in maize [17,43] and aluminum chlorite stress/toxicity in wheat [18].…”

Section: Discussionmentioning

confidence: 99%

Application of Zinc Oxide Nanoparticles and Plant Growth Promoting Bacteria Reduces Genetic Impairment under Salt Stress in Tomato (Solanum lycopersicum L. ‘Linda’)

et al. 2020

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Salinity is an edaphic stress that dramatically restricts worldwide crop production. Nanomaterials and plant growth-promoting bacteria (PGPB) are currently used to alleviate the negative effects of various stresses on plant growth and development. This study investigates the protective effects of different levels of zinc oxide nanoparticles (ZnO-NPs) (0, 20, and 40 mg L−1) and PGPBs (no bacteria, Bacillus subtilis, Lactobacillus casei, Bacillus pumilus) on DNA damage and cytosine methylation changes in the tomato (Solanum lycopersicum L. ‘Linda’) seedlings under salinity stress (250 mM NaCl). Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) and Randomly Amplified Polymorphic DNA (RAPD) approaches were used to analyze changes in cytosine methylation and to determine how genotoxic effects influence genomic stability. Salinity stress increased the polymorphism rate assessed by RAPD, while PGPB and ZnO-NPs reduced the adverse effects of salinity stress. Genomic template stability was increased by the PGPBs and ZnO-NPs application; this increase was significant when Lactobacillus casei and 40 mg L−1 of ZnO-NPs were used.A decreased level of DNA methylation was observed in all treatments. Taken together, the use of PGPB and ZnO-NPs had a general positive effect under salinity stress reducing genetic impairment in tomato seedlings.

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Effects of As2O3 on DNA methylation, genomic instability, and LTR retrotransposon polymorphism in Zea mays

Cited by 20 publications

References 45 publications

Estimation of genomic instability and DNA methylation due to aluminum (Al) stress in wheat ( Triticum aestivum L.) using iPBS and CRED-iPBS analyses

Estimation of genomic instability and DNA methylation due to aluminum (Al) stress in wheat ( Triticum aestivum L.) using iPBS and CRED-iPBS analyses

Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata

Application of Zinc Oxide Nanoparticles and Plant Growth Promoting Bacteria Reduces Genetic Impairment under Salt Stress in Tomato (Solanum lycopersicum L. ‘Linda’)

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