Application of Zinc Oxide Nanoparticles and Plant Growth Promoting Bacteria Reduces Genetic Impairment under Salt Stress in Tomato (Solanum lycopersicum L. ‘Linda’)

Abstract

Salinity is an edaphic stress that dramatically restricts worldwide crop production. Nanomaterials and plant growth-promoting bacteria (PGPB) are currently used to alleviate the negative effects of various stresses on plant growth and development. This study investigates the protective effects of different levels of zinc oxide nanoparticles (ZnO-NPs) (0, 20, and 40 mg L−1) and PGPBs (no bacteria, Bacillus subtilis, Lactobacillus casei, Bacillus pumilus) on DNA damage and cytosine methylation changes in the tomato (Solanum lycopersicum L. ‘Linda’) seedlings under salinity stress (250 mM NaCl). Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) and Randomly Amplified Polymorphic DNA (RAPD) approaches were used to analyze changes in cytosine methylation and to determine how genotoxic effects influence genomic stability. Salinity stress increased the polymorphism rate assessed by RAPD, while PGPB and ZnO-NPs reduced the adverse effects of salinity stress. Genomic template stability was increased by the PGPBs and ZnO-NPs application; this increase was significant when Lactobacillus casei and 40 mg L−1 of ZnO-NPs were used.A decreased level of DNA methylation was observed in all treatments. Taken together, the use of PGPB and ZnO-NPs had a general positive effect under salinity stress reducing genetic impairment in tomato seedlings.

Determination of genetic diversity in european cranberrybush (Viburnum opulus L.) genotypes based on morphological, phytochemical and ISSR markers

“…Different studies are carried out to determine genetic diversity in plants, both for breeding purposes and for the protection of the species. (Hosseinpour et al, 2020). These studies were generally made on morphological and phytochemical characters.…”

Green Synthesis of Zinc Oxide Nanoparticles: Fortification for Rice Grain Yield and Nutrients Uptake Enhancement

“…Via these modifications, it is expected to produce positive results in terms of both plant growth and the micronutrients assessed in fertilizers, which are increasingly quantitatively and qualitatively efficient in the agro-sector [ 16 , 17 ]. In particular, foliar treatment with Zn fertilizers as ZnO NPs achieved promising results in improving the nutritional efficiency of the grain under both natural and stress conditions [ 18 ], increasing the yield of grains, the amount of spikelets per spike, the number of tillers per hill, and the weight of 1000 grains [ 19 , 20 ]. They induce a dramatic increase of root and shoot length and mass in the leaf area index of up to 69% (both fresh and dry) when rice plants are exposed to 25, 50, 75, 100 and 150 mL of ZnO NPs [ 21 ].…”

Nanomaterials Induced Genotoxicity in Plant: Methods and Strategies

“…Hosseinpour et al [ 45 ] studied the effects of the application of ZnO NPs (0–40 mg L −1 ) and plant growth promoting bacteria on S. lycopersicum L. under salt stress, with particular regard to DNA damage and cytosine methylation changes. RAPD analysis has been performed to determine the effects of co-exposure to bacteria and ZnO NPs on tomato genomic DNA.…”