Effects of Applied DC Electric Field on Ligament Fibroblast Migration and Wound Healing

Chao, Pen-Hsiu Grace; Lu, Helen H.; Hung, Clark T.; Nicoll, Steven B.; Bulinski, J. Chloë

doi:10.1080/03008200701424451

Cited by 77 publications

(62 citation statements)

References 67 publications

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“…Electric fields and potentials induce distinct effects on cells and it has been proven that small applied electric fields can guide a variety of different cell types to move and migrate directionally such as corneal, epidermal and epithelial cells [38][39][40][41]; can modulate the phenotypes of vascular endothelial cells [42]; can regenerated nerve fibers [43] and are widely used in orthopedic practices, showing the improvement of ligament healing in vivo [44].…”

mentioning

confidence: 99%

Piezoelectric polymers as biomaterials for tissue engineering applications

Ribeiro

Sencadas

Correia

et al. 2015

Colloids and Surfaces B: Biointerfaces

403

338

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Tissue engineering often rely on scaffolds for supporting cell differentiation and growth. Novel paradigms for tissue engineering include the need of active or smart scaffolds in order to properly regenerate specific tissues. In particular, as electrical and electromechanical clues are among the most relevant ones in determining tissue functionality in tissues such as muscle and bone, among others, electroactive materials and, in particular, piezoelectric ones, show strong potential for novel tissue engineering strategies, in particular taking also into account the existence of these phenomena within some specific tissues, indicating their requirement also during tissue regeneration. This referee reports on piezoelectric materials used for tissue engineering applications. The most used materials for tissue engineering strategies are reported together with the main achievements, challenges and future needs for research and actual therapies. This review provides thus a compilation of the most relevant results and strategies and a start point for novel research pathways in the most relevant and challenging open questions. This referee reports on piezoelectric materials used for tissue engineering applications.The most used materials for tissue engineering strategies are reported together with the main achievements, challenges and future needs for research and actual therapies. This referee provides thus a compilation of the most relevant results and strategies and a start point for novel research pathways in the most relevant and challenging open questions.

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mentioning

confidence: 99%

Piezoelectric polymers as biomaterials for tissue engineering applications

Ribeiro

Sencadas

Correia

et al. 2015

Colloids and Surfaces B: Biointerfaces

403

338

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“…On the contrary, static EMF had an inhibitory effect on wound healing, which was reversed by pulsed EMF (Chao et al, 2007).…”

Section: Electromagnetic Fieldsmentioning

confidence: 91%

The Role of Physical Factors in Cell Differentiation, Tissue Repair and Regeneration

Monici¹,

Cialdai²

2012

Tissue Regeneration - From Basic Biology to Clinical Application

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“…Inhibition of raft and integrin polarization from Cav-1 knockdown cells suggested reciprocal interactions among raft, integrin, and Cav-1, and the integrity of all three components was necessary for EF-directed migration. Activation of integrin by extracellular matrix proteins has been shown to change integrin partitioning (35)(36)(37), stabilize lipid rafts (38), and modulate EFinduced motility and directionality (4,39). Integrin activation may also induce src signaling that phosphorylates Cav (40).…”

Section: Resultsmentioning

confidence: 99%

“…The electric field (EF) results in polarized cell organization and induces directional cell migration (galvanotaxis or electrotaxis), morphological changes (galvanotropism), and alterations in gene expression (4,5). In development, the electrical currents arise from regulation of ion channels that lead to ion flux and establish polarization and morphogenesis (1,6).…”

mentioning

confidence: 99%

Lipid rafts sense and direct electric field-induced migration

Lin

Tsao

Chen

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Endogenous electric fields (EFs) are involved in developmental regulation and wound healing. Although the phenomenon is known for more than a century, it is not clear how cells perceive the external EF. Membrane proteins, responding to electrophoretic and electroosmotic forces, have long been proposed as the sensing molecules. However, specific charge modification of surface proteins did not change cell migration motility nor directionality in EFs. Moreover, symmetric alternating current (AC) EF directs cell migration in a frequency-dependent manner. Due to their charge and ability to coalesce, glycolipids are therefore the likely primary EF sensor driving polarization of membrane proteins and intracellular signaling. We demonstrate that detergent-resistant membrane nanodomains, also known as lipid rafts, are the primary response element in EF sensing. The clustering and activation of caveolin and signaling proteins further stabilize raft structure and feedforward downstream signaling events, such as rho and PI3K activation. Theoretical modeling supports the experimental results and predicts AC frequency-dependent cell and raft migration. Our results establish a fundamental mechanism for cell electrosensing and provide a role in lipid raft mechanotransduction.

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Effects of Applied DC Electric Field on Ligament Fibroblast Migration and Wound Healing

Cited by 77 publications

References 67 publications

Piezoelectric polymers as biomaterials for tissue engineering applications

Piezoelectric polymers as biomaterials for tissue engineering applications

The Role of Physical Factors in Cell Differentiation, Tissue Repair and Regeneration

Lipid rafts sense and direct electric field-induced migration

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