Effects of apigenin and <i>tt</i>‐farnesol on glucosyltransferase activity, biofilm viability and caries development in rats

Koo, H.; Pearson, S.K.; Scott-Anne, Kathy; Abranches, Jacqueline; Cury, Jaime Aparecido; Rosalen, Pedro Luiz; Park, Y. K.; Marquis, Robert E.; Bowen, William H.

doi:10.1034/j.1399-302x.2002.170602.x

Cited by 125 publications

(155 citation statements)

References 51 publications

(80 reference statements)

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“…Si embargo se conoce que el propóleo cuenta con flavones como apigenin, tt-farnesol, compuestos que presentan acción antibacteriana y anticaries. 7 En el país sólo existe el análisis de propiedades antimicrobianas realizado dentro del estudio de estandarización; 11 el cual comprobó la actividad antibacteriana contra Staphylococcus aureus, C. albicans y Asperguillus níger, por lo que esta investigación representa el primer paso para presentar los efectos antibacterianos en la cavidad oral.…”

Section: Resultados Y Discusiónunclassified

“…4,5 Otros autores han estudiado fracciones y componentes del Propó-leo demostrando que la fracción H-fr y los flavones (apigenin y tt-farnesol) presentan alta actividad antibacteriana y poder inhibitorio de la Glucosil transferasa (GTF) con reducción de caries dental. 6,7 Por otro lado en estudios clínicos se ha comprobado que un colutorio a base de Propóleo no presentó actividad antiplaca significativa, pero su actividad antibacteriana fue semejante a la clorhexidina, 8 asimismo una crema dental con Propóleo redujo significativamente el promedio de piezas afectadas con caries dental; 9 otro estudio determinó que el Propó-leo tiene alta concentración de calcio (Ca 2+ ) y la dureza del esmalte mostró un aumento constante en proporción directa al aumento de concentración de la solución. 10 La geografía peruana y su diversa vegetación se prestan para el desarrollo de la apicultura, al punto que está en vías de ser una industria.…”

Section: Introductionunclassified

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Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei

Eguizábal

Nakata

2014

Odontol. sanmarquina

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ResumenCon el objeto de determinar la acción antibacteriana del extracto etanólico del propóleo peruano (EEPP) proveniente del Valle de Oxapampa (Pasco); mediante el método de difusión en Placa se usó las cepas Streptococcus mutans ATCC 25175 y Lactobacillus casei ATCC 393, para enfrentarlas a las soluciones: 0,8, 20 y 30 % v/v del EEPP, y compararlas a los testigos Clorhexidina 0,12 % y alcohol 70 %. Se determinó que la acción antibacteriana del EEPP contra S. mutans muestra una mayor tendencia de actividad inversamente proporcional a su concentración, que en el caso del L. casei; tal acción antibacteriana en las concentraciones 0,8, 20 y 30 % es significativa en comparación al testigo negativo; así mismo la acción contra S. mutans es mayor que en L. casei; siendo significativas en las concentraciones de 0,8 y 20 %; y también la acción antibacteriana del EEPP al 0,8 % es mayor que la acción de la Clorhexidina, tanto para S. mutans y L. casei. Se concluye que EEPP en solución al 0,8 % tiene una mejor acción antibacteriana contra S. mutans y L. casei que la Clorhexidina al 0,12 %. AbstractWith the intention of determining the antibacterial action of the ethanolic extract of Peruvian propolis (EEPP) originally from Oxapampa's Valley (Pasco); using the method of diffusion in Plate the vine-stocks Streptococcus mutans ATCC 25175 and Lactobacillus casei ATCC 393 were used, and faced to the solutions: 0,8, 20 and 30 % v/v of the EEPP, and compared them to the witnesses Clorhexidine 0,12 % and alcohol 70 %. It was determined that the antibacterial action of the EEPP against S. mutans shows a greater tendency of inversely proportional activity in its concentration, to that of L. casei case; such antibacterial action in concentrations 0,8, 20 and 30 % is significant in comparison to the negative witness; also the action against S. mutans is greater than in L. casei; being significant in the concentrations of 0,8 and 20 %; and also the antibacterial action of the EEPP to 0,8 % is greater than the action of Clorhexidine, as much for S. mutans and L. casei. One concludes that EEPP in solution to 0,8 % has a better antibacterial action against S. mutans and L. casei that the Clorhexidine to 0,12 %.

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Section: Resultados Y Discusiónunclassified

Section: Introductionunclassified

Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei

Eguizábal

Nakata

2014

Odontol. sanmarquina

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“…10,15,16,23,32 In these studies, ethanolic extracts of propolis, or its bioactive fractions, were applied topically to the molars of rats twice a day for a period of 4 to 5 weeks. Overall, the authors observed anti-caries activity similar to that of Duraphat ® .…”

Section: Discussionmentioning

confidence: 99%

The anti-caries activity and toxicity of an experimental propolis-containing varnish

et al. 2017

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Abstract:We investigated the anti-caries effects of an experimental propolis varnish in vivo, and further tested its toxicity against fibroblasts. Fifty-six SPF female Wistar rats were infected with Streptococcus mutans UA159 (SM) and allocated into four groups (n = 14/group): G1, propolis varnish (15%/PV); G2, chitosan varnish (CV/vehicle); G3, gold standard (GS/Duraphat ® ); and G4, untreated. The animals received a single varnish application on their molars and were submitted to a high cariogenic challenge (Diet-2000, 56% sucrose, and 5% sucrose-added water, ad libitum) for 4 weeks. Total cultivable microbiota and SM were counted, and smooth-surface and sulcal caries were scored. PV, CV and GS cytotoxic effects were tested against fibroblasts. The data were analyzed using ANOVA with the TukeyKramer test (p ≤ 0.05). Total microbiota and SM counts did not differ among the treatments (p = 0.78), or in relation to the untreated group (p = 0.52). PV reduced development of smooth-surface enamel caries compared with the untreated group (p = 0.0018), with no significant difference from GS (p = 0.92); however, the PV effects were no longer observed when the dentin was affected. Neither PV nor GS prevented enamel sulcal lesion onset, but GS significantly reduced the severity of dentinal sulcal lesions (p < 0.0001). No significant difference was observed in fibroblast viability between PV and GS (p < 0.0001).In conclusion, PV prevented smooth-surface enamel caries and showed low cell toxicity. Nevertheless, due to the high cariogenic challenge, its effects were not sustained throughout the experiment. Further studies are encouraged to establish a protocol to sustain the long-term anticaries activity of PV in the oral cavity.

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“…Topical applications of tt-farnesol (1 min exposure, twice daily) diminished the EPS content and development of single-species S. mutans biofilms on saliva-coated hydroxyapatite (sHA) surfaces, and reduced the severity of smooth-surface caries in rats [10]. However, treatment is not lethal to S. mutans biofilms in vitro, did not inhibit glucosyltransferases activity, and tt-farnesol displayed minimal effects on the viability of the oral flora populations in vivo [10,[12][13]. It is possible that tt-farnesol may cause acid sensitization and affect intracellular metabolism of S. mutans by disrupting the bacterial membrane function due to its fatty acid-like structure and lipophilic characteristics [10,12]; consistent with reduction of IPS accumulation in S. mutans biofilms treated with tt-farnesol [11,13].…”

mentioning

confidence: 99%

“…However, treatment is not lethal to S. mutans biofilms in vitro, did not inhibit glucosyltransferases activity, and tt-farnesol displayed minimal effects on the viability of the oral flora populations in vivo [10,[12][13]. It is possible that tt-farnesol may cause acid sensitization and affect intracellular metabolism of S. mutans by disrupting the bacterial membrane function due to its fatty acid-like structure and lipophilic characteristics [10,12]; consistent with reduction of IPS accumulation in S. mutans biofilms treated with tt-farnesol [11,13]. We hypothesize that tt-farnesol can directly affect S. mutans physiology by damaging the cell-membrane, and thereby disrupt its ability to compete and become dominant in a mixed-species biofilm environment.…”

mentioning

confidence: 99%

Influences of trans‐trans farnesol, a membrane‐targeting sesquiterpenoid, on Streptococcus mutans physiology and survival within mixed‐species oral biofilms

et al. 2011

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Trans-trans farnesol (tt-farnesol) is a bioactive sesquiterpene alcohol commonly found in propolis (a beehive product) and citrus fruits, which disrupts the ability of Streptococcus mutans (S. mutans) to form virulent biofilms. In this study, we investigated whether tt-farnesol affects cell-membrane function, acid production and/or acid tolerance by planktonic cells and biofilms of S. mutans UA159. Furthermore, the influence of the agent on S. mutans gene expression and ability to form biofilms in the presence of other oral bacteria (Streptococcus oralis (S. oralis) 35037 and Actinomyces naeslundii (A. naeslundii) 12104) was also examined. In general, tt-farnesol (1 mmol· L -1 ) significantly increased the membrane proton permeability and reduced glycolytic activity of S. mutans in the planktonic state and in biofilms (P<0.05). Moreover, topical applications of 1 mmol· L -1 tt-farnesol twice daily (1 min exposure/treatment) reduced biomass accumulation and prevented ecological shifts towards S. mutans dominance within mixed-species biofilms after introduction of 1% sucrose. S. oralis (a non-cariogenic organism) became the major species after treatments with tt-farnesol, whereas vehicle-treated biofilms contained mostly S. mutans (>90% of total bacterial population). However, the agent did not affect significantly the expression of S. mutans genes involved in acidogenicity, acid tolerance or polysaccharide synthesis in the treated biofilms. Our data indicate that tt-farnesol may affect the competitiveness of S. mutans in a mixed-species environment by primarily disrupting the membrane function and physiology of this bacterium. This naturally occurring terpenoid could be a potentially useful adjunctive agent to the current anti-biofilm/anti-caries chemotherapeutic strategies.

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Effects of apigenin and tt‐farnesol on glucosyltransferase activity, biofilm viability and caries development in rats

Cited by 125 publications

References 51 publications

Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei

Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei

The anti-caries activity and toxicity of an experimental propolis-containing varnish

Influences of trans‐trans farnesol, a membrane‐targeting sesquiterpenoid, on Streptococcus mutans physiology and survival within mixed‐species oral biofilms

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