Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei

Abstract

ResumenCon el objeto de determinar la acción antibacteriana del extracto etanólico del propóleo peruano (EEPP) proveniente del Valle de Oxapampa (Pasco); mediante el método de difusión en Placa se usó las cepas Streptococcus mutans ATCC 25175 y Lactobacillus casei ATCC 393, para enfrentarlas a las soluciones: 0,8, 20 y 30 % v/v del EEPP, y compararlas a los testigos Clorhexidina 0,12 % y alcohol 70 %. Se determinó que la acción antibacteriana del EEPP contra S. mutans muestra una mayor tendencia de actividad inversamente proporcional a su concentración, que en el caso del L. casei; tal acción antibacteriana en las concentraciones 0,8, 20 y 30 % es significativa en comparación al testigo negativo; así mismo la acción contra S. mutans es mayor que en L. casei; siendo significativas en las concentraciones de 0,8 y 20 %; y también la acción antibacteriana del EEPP al 0,8 % es mayor que la acción de la Clorhexidina, tanto para S. mutans y L. casei. Se concluye que EEPP en solución al 0,8 % tiene una mejor acción antibacteriana contra S. mutans y L. casei que la Clorhexidina al 0,12 %. AbstractWith the intention of determining the antibacterial action of the ethanolic extract of Peruvian propolis (EEPP) originally from Oxapampa's Valley (Pasco); using the method of diffusion in Plate the vine-stocks Streptococcus mutans ATCC 25175 and Lactobacillus casei ATCC 393 were used, and faced to the solutions: 0,8, 20 and 30 % v/v of the EEPP, and compared them to the witnesses Clorhexidine 0,12 % and alcohol 70 %. It was determined that the antibacterial action of the EEPP against S. mutans shows a greater tendency of inversely proportional activity in its concentration, to that of L. casei case; such antibacterial action in concentrations 0,8, 20 and 30 % is significant in comparison to the negative witness; also the action against S. mutans is greater than in L. casei; being significant in the concentrations of 0,8 and 20 %; and also the antibacterial action of the EEPP to 0,8 % is greater than the action of Clorhexidine, as much for S. mutans and L. casei. One concludes that EEPP in solution to 0,8 % has a better antibacterial action against S. mutans and L. casei that the Clorhexidine to 0,12 %.

Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm

“…The CEs of propolis with the largest zones of inhibition were those from apiaries in Chontabamba, Saylla, Yura, and Oxapampa, with the last being widely studied and showing similar effects in previous studies (Alemán et al, 2018;Eguizábal & Nakata, 2014;Tovalino & Contreras, 2010). The boundless vegetation of the Oxapampa valley, where species such as Dictyocaryum lamarckianum (palm tree), Juglans neotropica (Andean walnut), Cyrtocymura scorpioides, Vernonanthura patens (Kunth), Baccharis latifolia, Cecropia spp., Inga spp., and Pinus tecunumanii (timber tree), combined with the tropical climate of this area (Muñoz & Del Pilar, 2015), offer a variety of active components that are transported by bees to their hives in the form of propolis (Fernández-Calderón et al, 2020;Oki nczyc et al, 2020;Rivera & Mesía, 2009).…”

Antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro oral biofilm

“…The CEs of propolis with the largest zones of inhibition were those from apiaries in Chontabamba, Saylla, Yura, and Oxapampa, with the last being widely studied and showing similar effects in previous studies ( Alemán et al ., 2018 ; Eguizábal & Nakata, 2014 ; Tovalino & Contreras, 2010 ). The boundless vegetation of the Oxapampa valley, where species such as Dictyocaryum lamarckianum (palm tree), Juglans neotropica (Andean walnut), Cyrtocymura scorpioides , Vernonanthura patens ( Kunth), Baccharis latifolia , Cecropia spp., Inga spp., and Pinus tecunumanii (timber tree), combined with the tropical climate of this area ( Muñoz & Del Pilar, 2015 ), offer a variety of active components that are transported by bees to their hives in the form of propolis ( Fernández-Calderón et al ., 2020 ; Okińczyc et al ., 2020 ; Rivera & Mesía, 2009 ).…”

Use and Effectiveness of Propolis on Chronic Periodontitis: a Systematic Review

“…Because there are changes in the oral environment or under host conditions, some bacteria tend to colonize and give rise to various oral pathologies mostly related with inflammation and infection (18). The use of propolis in dentistry has been studied for a long time; there are several studies mentioning that propolis extract can inhibit the growth of Gram-positive and Gram-negative bacteria, comparing its efficacy with some antibacterial drugs such as Ampicillin and Tetracycline, or even with antiseptics such as 0.12% Chlorhexidine (19,20). Regarding the prevention of oral diseases, propolis has been employed for its anticariogenic potential and the reduction of the accumulation of dentobacterial plaque.…”