Effects of antioxidants and antifreeze proteins on cryopreservation of blue catfish (Ictalurus furcatus) spermatogonia

Abualreesh, Muyassar H.; Myers, Jaelen N.; Gurbatow, Jeremy; Johnson, Andrew; Xing, De; Wang, Jinhai; Li, Shangjia; Coogan, Michael; Vo, Khoi; Husseini, Nour El; Dunham, Rex A.; Butts, Ian A. E.

doi:10.1016/j.aquaculture.2020.735966

Cited by 13 publications

(10 citation statements)

References 42 publications

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“…2019a; Abualreesh et al. 2020, 2021). Although still in its preliminary stages, successful vitrification of ovarian tissue has been reported in Zebrafish (Marques et al.…”

Section: Discussionmentioning

confidence: 99%

“…2018; Abualreesh et al. 2020, 2021). Both ovaries were dissected, weighed, and gonadosomatic index (gonad weight divided by body weight × 100) was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…The methods for calculating the postthaw oogonia cells/mL and viability recovery index (% of live cells) from each treatment were adopted from previous studies (Abualreesh et al. 2020, 2021; Hettiarachchi et al. 2020).…”

Section: Methodsmentioning

confidence: 99%

“…2020) as well as antioxidants and antifreeze proteins (Abualreesh et al. 2021) have been assessed on postthaw recovery of type A spermatogonia. Nevertheless, we cannot be certain that these specifications can be applied to ovarian cells of the same species and what adjustments need to be made to the protocol.…”

mentioning

confidence: 99%

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Development of an Effective Cryopreservation Protocol for Blue Catfish Oogonia

et al. 2021

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Long‐term storage of oogonia and germ‐line stem cells provides an alternative to the limitations associated with cryopreserving eggs of important fish species. These cell types are less vulnerable to the stresses of freezing. Cryopreservation has enormous potential for aquaculture advancement, but protocols must be developed for each species and cell type since its success hinges on various input factors. Blue Catfish Ictalurus furcatus were selected as the test species in this study because of the need to improve fry production of Blue Catfish ♂ × Channel Catfish I. punctatus ♀ hybrids, which can be facilitated by storing oogonia in gene banks. Our objective was to develop a freezing protocol for oogonia of this species. We tested different permeating and nonpermeating cryoprotectants, concentrations of these agents, and freezing rates. We proved that all three factors influenced postthaw recovery of oogonia. Of the permeating cryoprotectants, 1.0 M dimethyl sulfoxide resulted in the most live cells with the highest viability percentages, and adding 0.2 M lactose with 10% egg yolk further improved the results. There were also specific interactions in which the effects of concentration and freezing rate varied among the cryoprotectant treatments. The most effective freezing rate was −1.0°C/min, and cell viability was reduced at −2.5°C/min and −5.0°C/min. From these results, we propose adding 1.0 M dimethyl sulfoxide with 0.2 M lactose and 10% egg yolk to cryomedia and freezing it at a rate of −1.0°C/min. By developing a cryopreservation protocol for a commonly cultured catfish, this work may guide the development of protocols for other species of interest.

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“…2019a; Abualreesh et al. 2020, 2021). Although still in its preliminary stages, successful vitrification of ovarian tissue has been reported in Zebrafish (Marques et al.…”

Section: Discussionmentioning

confidence: 99%

“…2018; Abualreesh et al. 2020, 2021). Both ovaries were dissected, weighed, and gonadosomatic index (gonad weight divided by body weight × 100) was calculated.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Development of an Effective Cryopreservation Protocol for Blue Catfish Oogonia

et al. 2021

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“…It is also possible that improved cell survival following prolonged storage and rewarming is obtained if the method used by Kong et al [ 112 ] (where cryoprotectant was also added to the rewarming solution to inhibit/reduce ice recrystallization) is applied in subsequent research on organoids cryopreservation. In another study, a combination of AFPs and antioxidants (hypotaurine and catalase) improved the post-thaw survival and viability of spermatogonial stem cells (SSCs) from Ictalurus furcatus (blue catfish) [ 113 ]. Testing this approach on human SSCs is essential because improved cryopreservation of SSCs would imply progress in reproductive medicine and present young males with effective options to preserve fertility prior to commencing chemotherapy, radiotherapy, immunotherapy and other treatments that would impact adversely on sperm production.…”

Section: Applications Of Afps In Cryopreservationmentioning

confidence: 99%

Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking

Ekpo

Xie

et al. 2022

IJMS

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Antifreeze proteins (AFPs) or thermal hysteresis (TH) proteins are biomolecular gifts of nature to sustain life in extremely cold environments. This family of peptides, glycopeptides and proteins produced by diverse organisms including bacteria, yeast, insects and fish act by non-colligatively depressing the freezing temperature of the water below its melting point in a process termed thermal hysteresis which is then responsible for ice crystal equilibrium and inhibition of ice recrystallisation; the major cause of cell dehydration, membrane rupture and subsequent cryodamage. Scientists on the other hand have been exploring various substances as cryoprotectants. Some of the cryoprotectants in use include trehalose, dimethyl sulfoxide (DMSO), ethylene glycol (EG), sucrose, propylene glycol (PG) and glycerol but their extensive application is limited mostly by toxicity, thus fueling the quest for better cryoprotectants. Hence, extracting or synthesizing antifreeze protein and testing their cryoprotective activity has become a popular topic among researchers. Research concerning AFPs encompasses lots of effort ranging from understanding their sources and mechanism of action, extraction and purification/synthesis to structural elucidation with the aim of achieving better outcomes in cryopreservation. This review explores the potential clinical application of AFPs in the cryopreservation of different cells, tissues and organs. Here, we discuss novel approaches, identify research gaps and propose future research directions in the application of AFPs based on recent studies with the aim of achieving successful clinical and commercial use of AFPs in the future.

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Effect of Seasonality for Optimization of Germ Cell Extraction from Mature Blue Catfish

et al. 2023

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Xenogenesis is an innovative tool for hybrid catfish (female Channel Catfish Ictalurus punctatus × male Blue Catfish I. furcatus) seed production, accomplished by transplanting undifferentiated germ cells derived from a donor diploid fish into a sterile recipient, which then enables recipient fish to produce donor‐derived gametes. There is potential to collect donor‐derived germ cells from mature fish during certain times of the year depending upon seasonal temperature and serum sex steroid hormonal fluctuations. The objective of this study was to evaluate seasonal variations in germ cell counts and serum sex steroid hormonal profiles in mature Blue Catfish. Mature fish were collected monthly over the full annual cycle to quantify the number of live germ cells (spermatogonia A, oogonia), viability of germ cells, and levels of serum sex steroid hormones, including testosterone, 11‐ketotestosterone, and 17β estradiol. Extracted spermatogonia A counts were highest from April to June, whereas a significant decline was detected from July to November. Extracted live oogonia counts were highest in April and gradually decreased to zero over the months of May to August. Seasonal variations in serum testosterone, 11‐ketotestosterone, and 17β estradiol followed a similar pattern as the live spermatogonia A and oogonia counts. Even though spermatogonia A counts were relatively lower in mature than in immature Blue Catfish males, extracting spermatogonia A from mature Blue Catfish males during April to June provides an added advantage to the process of artificial fertilization, as it is required to sacrifice these fish to collect sperm.

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Effects of antioxidants and antifreeze proteins on cryopreservation of blue catfish (Ictalurus furcatus) spermatogonia

Cited by 13 publications

References 42 publications

Development of an Effective Cryopreservation Protocol for Blue Catfish Oogonia

Development of an Effective Cryopreservation Protocol for Blue Catfish Oogonia

Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking

Effect of Seasonality for Optimization of Germ Cell Extraction from Mature Blue Catfish

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