Effects of Antimicrobial Photodynamic Therapy on Organic Solution and Root Surface In Vitro

Abstract

Antimicrobial photodynamic therapy (a-PDT) is attracting attention as a new form of dental treatment. While it is primarily applied to produce an antibacterial effect, it decreases lipopolysaccharide (LPS) and protease activity. Here, we evaluated differences in the antibacterial activity of a-PDT on three types of bacteria and the effects on the organic substances (i.e., albumin and LPS). Furthermore, we investigated the effects of a-PDT on root surfaces. A FotoSan630® and toluidine blue were used to perform a-PDT in this study. We measured its antimicrobial activity against Porphyromonas gingivalis, Streptococcus mutans, and Enterococcus faecalis. Antimicrobial testing revealed strong antimicrobial action and P. gingivalis, E. faecalis, and S. mutans were almost undetectable after 50, 120, and 100 s, respectively. In organic resolution tests, albumin was significantly decreased from 1 min after a-PDT application onward, while LPS significantly decreased at 5 min after the application. The root surfaces after a-PDT were confirmed to be cleaner than the controls without suffering any damage. Depending on the bacterial species, a-PDT exhibited antimicrobial activity against various types of bacteria and sensitivity differed. Moreover, we reported that a-PDT resolves protein and LPS, enabling the formation of a healthy root surface without any damage.

Antimicrobial photodynamic therapy against oral biofilm: influencing factors, mechanisms, and combined actions with other strategies

“…A recent investigation pointed out that some PSs could interact with LPS, modify the structure of LPS, and alter the biological effects in cells, which may provide a different insight into the treatments not only in dentistry but also in general health (Couto et al, 2021). The result was in line with the findings of Matsushima et al (2021) who observed that aPDT treatment could lead to LPS decrease, organic resolution, organic debris removal, and root surface cleaning. Another study isolated inflamed human gingival fibroblasts (HGF) and treated them with MB plus light irradiation.…”

Hipoglicemia neonatal e a atuação do enfermeiro: uma revisão de literatura

“…Além dessas vantagens a Terapia Fotodinâmica é capaz de inativas as endotoxinas LPS (lipopolissacarídeo), que estão presentes em bactérias gram-negativas, são agentes causadores de lesões periapicais. E tem capacidade de penetrar os corantes fotossensibilizadores no interior dos túbulos dentinários infectados (Matsushima et al 2021).…”

Recent Advances in Bio‐Compatible Oxygen Singlet Generation and Its Tumor Treatment

“…[334][335][336] Therefore, PDT can not only be used for dental bacteria inactivation. [337,338] and wound healing in dentistry, [339] more importantly, it can provide the dawn of cure for patients with MDR bacteria infection.…”