Effects of Antibiotic Diluent Additives on the Motility Parameters and Morphological Integrity of Boar Sperm During Six Days of Storage

Abstract

Summary The purpose of this paper is to examine the effect of antibiotic diluent additives on the motility and morphological integrity of diluted fresh boar semen during a six-day storage period. A total of 60 insemination doses, originating from two Landrace boars, were examined and allocated to control (C, n=30, diluted with BTS) and experimental groups (E, n=30, diluted with BTS upon antibiotic addition). The treatment applied exerted positive effects on the preservation of progressive motility, percentage of live sperm and HOS test results (70.24 vs. 66.53%, 71.54 vs. 69.77%, 67.35 vs. 64.17% and 64.10 vs. 54.26%;91.15 vs. 90.02%, 88.38 vs. 85.55%, 81.50 vs. 76.13% and 74.53 vs. 68.72%; and 93.35 vs. 92.40%, 91.04 vs. 88.02 %, 84.67 vs. 78.15% and 77.27 vs. 69.44% HOS+ sperm for the 1st, 3rd, 5th and 6th day of storage, respectively). The results obtained indicate that the treatment applied has a favourable effect on preserving the quality parameters of diluted fresh boar semen during storage, resulting most likely from a reduction of bacterial contamination.