Effects of Angelicae Gigantis Radix pharmacopuncture on Nitric Oxide and Prostaglandin E<sub>2</sub>Production in Macrophage

Choi, You Jin; Roh, Jeong Du

doi:10.3831/kpi.2011.14.3.081

Cited by 5 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In spite of this, no clinical reviews on the effects of Eun-Bi San pharmacopuncture have been published, but there have been many studies on the component herbs of this pharmacopuncture have been published. Pharmacopuncture of Angelicae gigantis radix , which is a component of Eun-Bi San pharmacopuncture, has an anti-inflammatory property related to its inhibition of nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS) induced macrophages, so it may have a therapeutic potential for the treatment of various inflammatory diseases [ 5 ]. Angelicae gigantis radix pharmacopuncture also has inhibitory effect on elastase activity as well as an antioxidant effect on NO and 1.1-diphenyl- 2-picrylhydrazyl (DPPH) [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Eun-bi San Pharmacopuncture Injection in the Muscle of Rats

Lee¹,

Jeong²,

Cha³

et al. 2015

Journal of Pharmacopuncture

View full text Add to dashboard Cite

Objectives:This study was carried out to analyze the single-dose toxicity of Eun-Bi San pharmacopuncture injected into the muscle of Sprague-Dawley (SD) rats.Methods:All experiments were performed at Biotoxtech, an institution certified to conduct non-clinical studies under the Good Laboratory Practice (GLP) regulations. Six week old SD rats reared by ORIENTBIO were chosen for this pilot study. The reason SD rats were chosen is that they have been widely used in safety tests in the field of medicine, so the results can be easily compared with many other databases. The Eun-Bi San pharmacopuncture was made in a clean room at the Korean Pharmacopuncture Institute (KPI, K-GMP). The constituents of the Eun-Bi San pharmacopuncture are Angelicae gigantis radix, Strychni semen and Glycyrrhizae radix. These were extracted at low temperature and low pressure in an aseptic room at the KPI. Doses of Eun- Bi San pharmacopuncture, 0.25, 0.5 and 1.0 mL, were administered to the experimental groups, and a dose of normal saline solution, 1.0 mL, was administered to the control group. This study was performed under the approval of the Institutional Animal Ethics Committee of Biotoxtech Co., Ltd.Results:No deaths or abnormalities occurred in any of the four groups. No significant changes in weight, hematological parameters or clinical chemistry between the control group and the experimental groups were observed. To determine if abnormalities existed in any organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues.Conclusion:The results of this study suggest that treatment with Eun-Bi San pharmacopuncture is relatively safe and that its clinical use may be beneficial. Further evaluations and studies on this subject will be needed to provide more concrete evidence in support of these conclusions.

show abstract

Section: Discussionmentioning

confidence: 99%

Toxicity of Eun-bi San Pharmacopuncture Injection in the Muscle of Rats

Lee¹,

Jeong²,

Cha³

et al. 2015

Journal of Pharmacopuncture

View full text Add to dashboard Cite

show abstract

“…Persistent inflammatory response can result in myocarditis, chronic arthritis, glomerulonephritis, insulin dependent diabetes mellitus, and irritable bowel 22) . Therefore in recent various study, looking for ways to treat inflammation by effectively suppress the production of NO [23][24][25] .…”

Section: Discussionmentioning

confidence: 99%

Anti-oxidant and Anti-inflammatory Effects of <i>Acanthopanacia Cortex</i> Hot Aqueous Extract on Lipopolysaccharide(LPS) Simulated Macrophages

Roh

2014

The Acupuncture

View full text Add to dashboard Cite

Objectives : This study is to investigate the effects of Acanthopanacis Cortex hot aqueous extract on nitric oxide(NO), prostaglandin E2(PGE2) production and DPPH(1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity in macrophages. Methods : Acanthopanacis Cortex(200 g) was heated at 100 ℃ with distilled water(2 L) for 4hrs. The extract was filtered and concentrated to 100 ㎖ using a rotary evaporator and was frozen at-80 ℃, then was freeze-dried. The RAW 264.7 macrophages were subcultured. In order to evaluate cytotoxicity, MTT assay was performed. Experimental groups were divided into five(control, AC 25, 50, 100 and 200 ㎍/㎖) and we measured cytotoxicity. The concentrations of NO were preprocessed by Griess assay. The RAW 264.7 macrophages was pretreated by 10 ㎍/㎖ LPS and experimental groups were divided into five and we measured NO production. The concentrations of PGE2 were measured by enzyme immunoassay. The RAW 264.7 macrophages was pretreated by 10 ㎍/㎖ LPS. Experimental groups were divided into five and we measured PGE2 production. Antioxidant activity was measured by the DPPH method. experimental groups were divided into four(AC 25, 50, 100 and 200 ㎍/㎖) and we measured DPPH radical scavenging activity. Results : 1. Viability of RAW 264.7 macrophages did not significantly decrease in 25, 50 and 100 ㎍/㎖ Acanthopanacis Cortex hot aqueous extract compared to control group. 2. NO production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 ㎍/㎖ Acanthopanacis Cortex hot aqueous extract compared to control group. 3. PGE2 production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 ㎍/㎖ Acanthopanacis Cortex hot aqueous extract compared to control group. 4. DPPH radical scavenging capability of Acanthopanacis Cortex hot aqueous extract in RAW 264.7 macrophages had the high level in 100, 200 ㎍/㎖. Conclusion : According to the results, Acanthopanacis Cortexx hot aqueous extract has ability to suppress NO, PGE2 production and improve DPPH free radical scavenging activity. So Acanthopanacis Cortex hot aqueous extract may have an anti-inflammation effect and antioxidant activity.

show abstract

“…등 염증 매개 물질의 생성 억제 67,68) , IL-4의 분비 촉진 69) , 염증성 단백질의 발현 변화 70 57,59,61) . 또한 파골 세포의 형성 을 억제하는 작용이 있는 白朮 72) ,…”

Section: %unclassified

The Effect of Orally Administered Herbal Medicines in Collagen, LPS, CFA Induced Rheumatoid Like Arthritis Animal Model: A Systematic Review of Animal Study Articles Published in Korea after 2008

Won¹,

Chaeheun²,

Song³

et al. 2017

J Korean Med Rehabil

View full text Add to dashboard Cite

Objectives This study was designed to review animal studies about the efficacy of herbal medicine for Rheumatoid arthritis animal model which was published in Korea after 2008. We also systematically investigated the reporting quality of the animal studies. Methods We systematically searched original articles in 8 databases since 2008. And manual searching was conducted through 10 Korean medicine journals from 2008. Studies were included if they used animal experimental model(s) with orally administered herbal medicine. Data were extracted regarding animal model, rheumatoid arthritis indicator and detail of intervention. Reporting quality of each study was also assessed by the STARA and ARRIVE guidelines. Results Nine hundred two articles were screened. Finally, 35 articles were included. 35 studies all showed that the herbal medicine used in the studies has significant effect on alleviating the macroscopic indicators, hematological indicators, histological indicators, genetic indicators, osteological indicator of Rheumatoid Arthritis and others. Species of animals was reported in 100% while ventilation and noise were reported in 0% in STRASA guidelines. Ethical statement was reported in 42.9%, experimental animals and sample size were reported in 24%, 29% and there was no study reporting funding. Conclusions These results suggest that herbal medicine might be effective treatment for Rheumatoid Arthritis. It should be improved by clinical studies. And there is a need for studying about efficacy and safety of each specific herbal medicine. And we should improve the Reporting quality of the animal studies published in Korea.

show abstract

Effects of Angelicae Gigantis Radix pharmacopuncture on Nitric Oxide and Prostaglandin E₂Production in Macrophage

Cited by 5 publications

References 6 publications

Toxicity of Eun-bi San Pharmacopuncture Injection in the Muscle of Rats

Toxicity of Eun-bi San Pharmacopuncture Injection in the Muscle of Rats

Anti-oxidant and Anti-inflammatory Effects of <i>Acanthopanacia Cortex</i> Hot Aqueous Extract on Lipopolysaccharide(LPS) Simulated Macrophages

The Effect of Orally Administered Herbal Medicines in Collagen, LPS, CFA Induced Rheumatoid Like Arthritis Animal Model: A Systematic Review of Animal Study Articles Published in Korea after 2008

Contact Info

Product

Resources

About

Effects of Angelicae Gigantis Radix pharmacopuncture on Nitric Oxide and Prostaglandin E2Production in Macrophage

Cited by 5 publications

References 6 publications

Toxicity of Eun-bi San Pharmacopuncture Injection in the Muscle of Rats

Toxicity of Eun-bi San Pharmacopuncture Injection in the Muscle of Rats

Anti-oxidant and Anti-inflammatory Effects of &lt;i&gt;Acanthopanacia Cortex&lt;/i&gt; Hot Aqueous Extract on Lipopolysaccharide(LPS) Simulated Macrophages

The Effect of Orally Administered Herbal Medicines in Collagen, LPS, CFA Induced Rheumatoid Like Arthritis Animal Model: A Systematic Review of Animal Study Articles Published in Korea after 2008

Contact Info

Product

Resources

About

Effects of Angelicae Gigantis Radix pharmacopuncture on Nitric Oxide and Prostaglandin E₂Production in Macrophage

Anti-oxidant and Anti-inflammatory Effects of <i>Acanthopanacia Cortex</i> Hot Aqueous Extract on Lipopolysaccharide(LPS) Simulated Macrophages