Effects of Andrographis paniculata extract and Andrographolide on hepatic cytochrome P450 mRNA expression and monooxygenase activities after in vivo administration to rats and in vitro in rat and human hepatocyte cultures

Pekthong, Dumrongsak; Blanchard, Nadège; Abadie, Catherine; Bonet, Alexandre; Heyd, Bruno; Mantion, Georges; Berthelot, Alain; Richert, Lysiane; Martin, Hélène

doi:10.1016/j.cbi.2008.10.054

Cited by 63 publications

(47 citation statements)

References 30 publications

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“…This is a possible explanation to the results found by Pan et al (2011), who concluded that the petroleum ether (PE) extract of Orthosiphon stamineus produces the greatest inhibitor effect on CYP2C19. At the time this experiment was published, there were no other studies on R. chalepensis extract in cytochromes available to compare our results; however, previous research reports on the study of other plants, such as Andrographis paniculata (Pekthong et al, 2009), Orthosiphon stamineus, Aframomum cuspidatum (Agbonon et al, 2010), and Centella asiatica (Pan et al, 2011) in cell culture. Centella asiatica was also studied in vivo by Kulthong et al, (2009).…”

Section: Discussionmentioning

confidence: 47%

“…CYP3A is expressed mostly in the liver and the gastrointestinal tract (Chen et al, 2009). People tend to combine different plant preparations with modern prescription drugs, in that they consider it safe to consume herbal derivatives; such combinations can produce plant-drug interactions (Pekthong et al, 2009). Ruta chalepensis (R. chalepensis) is native to southern Europe and the Eastern Mediterranean region, where it is known as Ruda; this plant is cultivated in the greater part of the American continent.…”

Section: Introductionmentioning

confidence: 99%

“…These enzymes are also charged with metabolizing thousands of bioactive compounds of plants with very diverse chemical structures (Laursen et al, 2011). These enzymes are the main mechanism for increased, diminished, or null bioavailability of herbal-based drugs and compounds when said enzymes and drugs are simultaneously administered (Pekthong et al, 2009;Wang et al, 2009). They are termed P450 because an absorbance peak is produced at 450 nm when treated with a reducer agent and linked with carbon monoxide (Pan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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In Vivo Effect of Ruta Chalepensis Extract on Hepatic Cytochrome 3a1 in Rats

Martínez-Pérez

Hernández-Terán

Serrano-Gallardo

2017

Afr J Tradit Complement Altern Med

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Background: Since the time when drugs began to be used, it became evident that they could produce a therapeutic effect, but also a clinical condition of toxicity or no effect at all on humans, despite using the same doses in different patients. Such untoward effects were termed "drug idiosyncrasy" and also "idiosyncratic drug effects", but the factors producing such diverse responses were never taken into account. Materials and Methods: Ruta chalepensis L. (fringed rue) is an herbaceous plant of the Rutaceae family used in traditional medicine due to its properties, such as its analgesic and antipyretic effects. This study used 25 male rats divided into five groups. Plant extract was administered to Groups 1 and 2 at doses of 100 and 30 mg/kg/day, respectively, for three days; Group 3 was administered 100 mg/kg/day of dexamethasone (DEX), as well as 100 mg/kg/day of Ruta chalepensis extract; Group 4 was administered 100 mg/kg/day of DEX and treated as positive control; Group 5 was treated as negative control and was administered a physiological solution. Twenty-four hours after the the last dose, the animals were sacrificed and their livers were extracted. Results: The aqueous extract of Ruta chalepensis, intraperitoneally administered, was able to induce cytochrome 3A1 in doses of 30 mg/kg/day, and a greater inducing effect occurs when the plant is co-administered in doses of 100 mg/kg/day with dexamethasone. Conclusion: This study suggests that aqueous extract of Ruta chalepensis can induce cytochrome 3a1. This study helps provide a better understanding of CYP3a regulation. Future in vitro work is needed to determine the compounds that produce the cytochrome modulation.

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Section: Discussionmentioning

confidence: 47%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vivo Effect of Ruta Chalepensis Extract on Hepatic Cytochrome 3a1 in Rats

Martínez-Pérez

Hernández-Terán

Serrano-Gallardo

2017

Afr J Tradit Complement Altern Med

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“…Namun, dalam banyak kasus kombinasi herbal dengan obat sintesis menyebabkan interaksi. Senyawa yang terdapat dalam herbal dapat berinteraksi dengan obat sintesis jika digunakan dalam waktu yang bersamaan [12]. Penelitian sebelumnya menunjukkan bahwa herba sambiloto merupakan inhibitor kompetitif enzim CYP3A4 pada manusia, dimana Glibenklamid dimetabolisme oleh enzim tersebut [13,14].…”

Section: Pendahuluanunclassified

Interaksi Ekstrak Sambiloto (Andrographis paniculata (Burm.F.)Ness) dengan Glibenklamid terhadap Ekspresi Gen CYP3A4 pada Kultur Sel HepG2

2017

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Diabetes mellitus is one of the chronic metabolic diseases characterized by hyperglycemia. Various therapies are done to overcome hyperglycemia, either with oral antidiabetic drugs or plants that have antidiabetic properties. Sambiloto (Andrographis paniculata (Burm.F.)Ness) is one of antidiabetic plants that is widely used by Indonesian people. However, in many cases, the combination of herbs with synthesis drugs causes interaction when used at the same time. This study was aimed to obtain interaction data of the sambiloto extract with glibenclamide towards CYP3A4 gene expression in HepG2 cell line. The results of this study showed a decreased in the expression of CYP3A4 gene in a single sample test as well as a combination sample at concentrations of 50 and 100 μg/mL for green chiretta extract, glibenclamide and its combination decreased CYP3A4 gene expression respectively 0,82, 0,70; 0,89, 0,53; 0,84, 0,72. It can be concluded there is a decreased in the expression of CYP3A4 gene along with the increase of sample concentration. ABSTRAK:Diabetes melitus (DM) merupakan salah satu penyakit metabolik kronis yang ditandai dengan hiperglikemia. Berbagai terapi dilakukan untuk mengatasi hiperglikemia, baik dengan menggunakan obat antidiabetes oral maupun tanaman herbal berkhasiat antidiabetes. Sambiloto (Andrographis paniculata (Burm.F.)Nees) merupakan salah satu herbal antidiabetes yang banyak dikonsumsi masyarakat Indonesia. Andrografolida merupakan zat aktif yang terdapat dalam sambiloto yang berperan sebagai agen antidiabetes. Namun, dalam banyak kasus kombinasi antara herbal dan obat sintesis menyebabkan interaksi jika digunakan pada waktu bersamaan. Penelitian ini bertujuan untuk memperoleh data interaksi ekstrak etanol herba sambiloto dengan glibenklamid terhadap ekspresi gen CYP3A4 pada kultur sel HepG2. Hasil penelitian ini menunjukkan bahwa terjadi penurunan ekspresi gen CYP3A4 pada pengujian sampel tunggal maupun kombinasi antara ekstrak etanol herba sambiloto dan glibenklamid dengan bertambahnya konsentrasi. Pada konsentrasi 50 dan 100 μg/mL sambiloto, glibenklamid, dan kombinasinya menyebabkan penurunan ekspresi gen CYP3A4 sekitar 0,82, 0,70; 0,89, 0,53; 0,84, 0,72. Berdasarkan hasil tersebut dapat disimpulkan terjadi penurunan ekspresi gen CYP3A4 dengan bertambahnya konsentrasi sampel.

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“…Then the liver was homogenized in 4 ml buffer per 1.0 g of liver. The homogenates were submitted for centrifugation at 4000 rpm for 30 min (Pekthong et al, 2008;Pekthong et al, 2009;Richert et al, 2002). Finally, microsomal samples containing supernatant collected and frozen at -80°C until analysis.…”

Section: Preparation Of Microsomesmentioning

confidence: 99%

In vitro Effect of Withania somnifera, Mucuna pruriens and Pausinystalia johimbe on Hepatic Cytochrome P450 in Rat

Sultana

Sultan

2018

Bangla Pharma J

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The effect of Withania somnifera, Mucuna pruriens and Pausinystalia johimbe extracts on hepatic cytochrome P450 enzyme CYP3A4 activities was studied using rat liver microsomes. CYP3A4-dependent testosterone 6β-hydroxylation activities were determined by ELISA. In the study, rats were treated with W. somnifera (0.5 g/kg/day), M. pruriens (0.5 g/kg/day) and P. johimbe (0.25 g/kg/day) extracts for 20 days. It was found that W. somnifera, M. pruriens and P. johimbe extracts showed potent to moderate inhibitory effect on CYP3A4 activities in rat liver microsomes, with IC 50 values of 18.01 ng/mL, 14.93 ng/mL and 21.03 ng/mL, respectively.

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Effects of Andrographis paniculata extract and Andrographolide on hepatic cytochrome P450 mRNA expression and monooxygenase activities after in vivo administration to rats and in vitro in rat and human hepatocyte cultures

Cited by 63 publications

References 30 publications

In Vivo Effect of Ruta Chalepensis Extract on Hepatic Cytochrome 3a1 in Rats

In Vivo Effect of Ruta Chalepensis Extract on Hepatic Cytochrome 3a1 in Rats

Interaksi Ekstrak Sambiloto (Andrographis paniculata (Burm.F.)Ness) dengan Glibenklamid terhadap Ekspresi Gen CYP3A4 pada Kultur Sel HepG2

In vitro Effect of Withania somnifera, Mucuna pruriens and Pausinystalia johimbe on Hepatic Cytochrome P450 in Rat

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