Effects of Anacardium humile leaf extracts on the development of gastrointestinal nematode larvae of sheep

Nery, Patrícia Silva; Nogueira, Flávia Aparecida; Martins, E.R.; Duarte, Eduardo Robson

doi:10.1016/j.vetpar.2010.03.043

Cited by 41 publications

(39 citation statements)

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“…Using the coproculture quantitative test, Nery et al (2010) reported that the aqueous extract of Anacardium humile leaves at 150 mg/mL was 97.3% effective, and the ethanol extract at 80 mg/mL was 99.6% effective, in LDI. In similar research, the LC 90 for LDI of aqueous extract of Caryocar Brasiliense was 53.19 mg/ml. )…”

Section: Resultsmentioning

confidence: 99%

“…Dried leaves were ground and stored at 5 ± 3°C until use. Extracts were obtained using the method of Nery et al (2010) with modifications. Ground dried leaves were incubated in a distilled water bath at 60°C for 60 min and hot filtered through a gauze funnel.…”

Section: Methodsmentioning

confidence: 99%

“…The leaf extract solution was prepared as described, standardised at 200 mg/mL, and diluted in sterile distilled water to 40, 60, 100 and 150 mg/mL. The extracts were used immediately in a larval development inhibition (LDI) test in quantitative culture (Borges 2003;Nery et al 2010). Unpreserved faeces collected directly from the rectal ampulla of each animal were pooled and divided into 2 g samples distributed among clean disposable cups.…”

Section: In Vitro Larval Development Inhibitionmentioning

confidence: 99%

“…The utilisation of these extracts for the reduction of anthelminthic-resistant populations of ovine nematodes may constitute a promising strategy in herds with high frequency of anthelminthic multiresistance (Nery et al 2010). Although G. americana is not included as an anthelminthic in folk use, it is a plant rich in tannins (Hernes & Hedges 2004) and therefore, the goal of the present study was to evaluate in vitro the anthelminthic efficacy of G. americana on the inhibition of larval development and eclosion for GIN-infecting sheep.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of aqueous extracts ofGenipa americanaL. (Rubiaceae) in inhibiting larval development and eclosion of gastrointestinal nematodes of sheep

Nogueira

Nery

Morais-Costa

et al. 2014

Journal of Applied Animal Research

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The anthelminthic efficacy in vitro of the aqueous extract of Genipa americana L. leaves was evaluated at final concentrations of 20, 30, 50, 75 and 100 mg (dry matter)/mL through egg hatching inhibition (EHI) tests and quantitative cultures. The observed lethal concentration LC 90 values for hatching and L3 development inhibition were 79.8 and 28.7 mg/mL, respectively. The extract was more effective in larval development inhibition (LDI) than in hatching inhibition. Phytochemical analysis revealed tannins and flavonoids in the extract. The utilisation of G. americana as an anthelminthic may represent a viable alternative to synthetic products, although further studies are needed to verify its in vivo efficacy and to assess its toxicity in sheep.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: In Vitro Larval Development Inhibitionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficacy of aqueous extracts ofGenipa americanaL. (Rubiaceae) in inhibiting larval development and eclosion of gastrointestinal nematodes of sheep

Nogueira

Nery

Morais-Costa

et al. 2014

Journal of Applied Animal Research

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“…Apesar do enorme potencial, alguns entraves sanitários diminuem a produtividade, sendo o principal deles as parasitoses gastrintestinais, que geram déficits econômicos significativos . A infecção por Haemonchus contortus é a principal doença parasitária, causando prejuízos devido a anorexia, apatia, aumento da conversão alimentar, diminuição da produção e perda de peso em animais infectados (Nery et al, 2010).O controle da enfermidade é, geralmente, realizado de forma intensiva, com o uso indiscriminado de anti-helmínticos comerciais, sem considerar os fatores epidemiológicos envolvidos. Este modelo de utilização favorece a seleção e a propagação da população parasitária resistente, além da deposição de resíduos no meio ambiente e aumento dos custos produtivos (Molento, 2005).…”

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Atividade ovicida e larvicida do extrato etanólico de Aloe vera L. sobre Haemonchus contortus

Sprenger¹

2015

RBCV

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ResumoO objetivo deste trabalho foi determinar a eficácia in vitro do extrato etanólico de Aloe vera L. frente a estágios de vida livre de Haemonchus contortus. O fitoterápico foi produzido com percolação da polpa do vegetal com álcool absoluto, a 25ºC, sendo posteriormente liofilizado. Foram realizados o teste de eclodibilidade de ovos (TEO) e o teste de desenvolvimento larval (TDL), com concentrações crescentes (0,78 a 100mg/mL) em seis repetições. Para analisar a composição química do fitoterápico, procedeuse a marcha fitoquímica completa, analisando qualitativamente a presença de fenóis, taninos, antocianinas, antocianidinas, leucoantocianidinas, estero ides, triterpenos, saponinas, resinas e alcaloides; além da quantificação de fenóis totais, teste de toxicidade frente Artemia salina e ensaio antioxidante pelo teste de redução do radical DPPH. O produto obtido a partir de A. vera apresentou eficácia de 94,35 ± 1,13% no TEO, já no TDL a eficácia foi de 76,03 ± 0,45%, ambos na concentração de 100mg/mL. Nas análises fitoquímicas, foram encontrados diversos compostos que podem ter contribuído com o efeito anti-helmíntico, tanto direta como indiretamente. Os dados da marcha fitoquímica, aliados aos resultados dose-dependentes obtidos nos testes in vitro evidenciam que o extrato produzido possui potencial para combater H. contortus. Novos estudos devem ser realizados buscando maximizar a eficácia deste extrato, uma vez que foram encontrados resultados excelentes nos testes realizados.Palavras-chave: Etnoveterinária, fitoterapia, infecção parasitária. AbstractThe aim of this study was to determine the in vitro efficacy of ethanolic extract of Aloe vera L. against Haemonchus contortus free-living stages. The herbal material was produced by pulp leaving the solution with ethanol at 25°C and latter the material was lyophilized. After this, the product was used for the egg hatch test (EHT) and the larval development test (LDT) in different concentrations (0.78 to 100mg/mL) with six replicates. A complete phytochemical screening was performed to analyze the materials' chemistry composition, screening qualitatively the presence of phenols, tannins, anthocyanins, anthocyanidins, leucoanthocyanidins, steroids, triterpenes, saponins, resins and alkaloids; beyond the total phenols quantification, brine shrimp toxicity test and DPPH radical reduction antioxidant test. A. vera extract had an efficacy of 94,35 ± 1,13% using the EHT and of 76,03 ± 0,45% when using the LDT test, both in concentration 100mg/mL. Phytochemical tests showed few chemical compounds that could exert anthelmintic properties. The results obtained with the biochemical tests together with the dose-dependent effect found in in vitro tests demonstrate that extract has the potential to be a good drug candidate against H. contortus. Further studies should be conducted to maximize the effectiveness of the herbal, because it demonstrated excellent results in experiments.Keywords: Ethnoveterinary, herbal medicine, parasitic infection. IntroduçãoA produção de pequ...

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Pharmacological Potential of Selected Natural Compounds in the Control of Parasitic Diseases

Hrčková

Velebný

2013

SpringerBriefs in Pharmaceutical Science &Amp; Drug Development

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The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

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Effects of Anacardium humile leaf extracts on the development of gastrointestinal nematode larvae of sheep

Cited by 41 publications

References 12 publications

Efficacy of aqueous extracts ofGenipa americanaL. (Rubiaceae) in inhibiting larval development and eclosion of gastrointestinal nematodes of sheep

Efficacy of aqueous extracts ofGenipa americanaL. (Rubiaceae) in inhibiting larval development and eclosion of gastrointestinal nematodes of sheep

Atividade ovicida e larvicida do extrato etanólico de Aloe vera L. sobre Haemonchus contortus

Pharmacological Potential of Selected Natural Compounds in the Control of Parasitic Diseases

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