Effects of an Intravenous Injection of Tetracosactid on Plasma Corticosteroid and Testosterone Levels in Unstressed Male Rabbits

Faulborn, K. W.; Fenske, M.; Pitzel, L.; König, Annemarie

doi:10.1530/acta.0.0910511

Cited by 25 publications

(13 citation statements)

References 6 publications

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“…To minimize the effects of trapping and handling on the values obtained, we performed an adrenocorticotrophic hormone (ACTH) challenge test (cf. Faulborn et al. 1979).…”

Section: Methodsmentioning

confidence: 99%

Fox Odour Increases Vigilance in European Rabbits: A Study under Semi‐Natural Conditions

2006

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The recognition of predator odours is a well‐known mechanism in many prey species which may lead to various behavioural and physiological responses. This has been shown for many mammal species under laboratory conditions, but efforts to validate the results in the field often have led to inconclusive results. We investigated the behavioural reactions and the physiological stress response of European rabbits (Oryctolagus cuniculus) to the odour of a mammalian predator (red fox, Vulpes vulpes) under semi‐natural conditions. The study was conducted on a rabbit population living in an outdoor enclosure of 2 ha. We compared the rates of vigilance and exploration, the time allocated to self‐directed behaviours, the home range sizes and the physiological responses of an experimental and a control group. Only animals from the experimental group were confronted with fox faeces. These animals increased their vigilance rate whereas the control animals did not respond. The increase did not differ between adult and subadult individuals. Furthermore, the experimental animals frequently approached the odour of the predator which might indicate an increase in investigative behaviour. Home‐range size, feeding and other self‐directed behaviours did not change in response to fox odour. Moreover, the animals of the experimental and the control group did not differ in serum corticosterone concentrations (measured after adrenocorticotrophic hormone challenge) that we determined in the beginning and in the end of the experiment. We suggest that the observed behavioural responses represent a low‐cost strategy for lowering the individual risk of predation.

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“…To minimize the effects of trapping and handling on the values obtained, we performed an adrenocorticotrophic hormone (ACTH) challenge test (cf. Faulborn et al. 1979).…”

Section: Methodsmentioning

confidence: 99%

Fox Odour Increases Vigilance in European Rabbits: A Study under Semi‐Natural Conditions

2006

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“…Cortisol and corticosterone are the major corticoste¬ roids of the pig adrenal cortex, with a predominance of cortisol (Whipp et al 1970). These two steroids were not separated, but were determined as total corticosteroids by a modified competitive protein-binding radioassay which has been described in detail by Faulborn et al (1979). The binding protein used (human plasma diluted 1:100 in 0.05 m phosphate buffer) significantly crossreacted with cortisol (100.09?).…”

Section: Methodsmentioning

confidence: 99%

“…This antiserum significantly cross-reacted only with dihydrotestosterone (44.0%), 5ct-androstane-3ß, 17ß-diol (3.0%), 5ß-androstane-3ß,17ß-diol (2.5%) and A-4-androstenedione (2.0%). The detail of this assay has been described in detail by Faulborn et al (1979). The coeffi¬ cient of variation within-assays was 13.0-22.0% and between-assays 15.0-22.1% in the range from 50.0-300.0 pg testosterone/tube.…”

Section: Methodsmentioning

confidence: 99%

Corticotropin- and lysine-vasopressin induced changes of plasma corticosteroids and testosterone in the adult male pig

Hahmeier

Fenske

Pitzel

et al. 1980

Acta Endocrinologica

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Intravenous injection of 10.0 \g=m\g/kgbody weight synthetic corticotropin (1-24 ACTH) into chronically cannulated boars resulted in significantly elevated plasma corticosteroid and testosterone levels between 20 and 140 min (corticosteroids) and 20\p=n-\80 min (testosterone) after injection. Administration of lysine-vasopressin (LVP) at doses of 0.1, 0.2 and 0.4 IU/kg body weight elicited a significant increase of plasma corticosteroids between 20 and 40 min after injection; on the other hand, plasma testosterone concentrations tended to fall when compared to pre-treatment levels. From our results it can be concluded that exogenously applied ACTH can effectively stimulate the release of corticosteroids and testosterone. Intravenous administration of LVP results in significantly, although not maximally increased plasma corticosteroid concentrations; the release of endogenous ACTH induced by LVP injection, on the other hand, appeared to be too small to stimulate testosterone release significantly.When administering short-acting corticotropin (ACTH) (Liptrap & Raeside 1975;Holtz et al. 1978) or cortisol (Liptrap & Raeside 1978) to pigs, significantly elevated plasma corticosteroid and testosterone levels were obtained. This indicates that both exogenous ACTH and cortisol exert a stimulatory effect upon testosterone secretion. We therefore decided to compare the effects of an elevation of endogenous ACTH on plasma corticosteroid and testosterone levels to that by iv ACTH injection. Endogenous ACTH was increased by injecting lysine-vasopressin which is known to pos¬ sess corticotropin-releasing activity in a number of mammalian species (for ref. see Yates & Maran 1974). Material and MethodsSeventeen adult male miniature pigs (Göttingen strain) with an average body weight of 38 kg (36-40 kg) were used. Thev were kept in individual pens in the same room under natural lighting conditions and fed a stand¬ ard diet containing 15.59? crude protein and 12.59? crude fibre. One week before test performance the animals were fitted with permanently indwelling cathe¬ ters leading to the external jugular vein. The catheter consisted of a silicone rubber tube (Silastic, Dow Corning; length: 40.0 cm, inner diameter: 1.57 mm, outer dia¬ meter: 3.18 mm) which ended inside the cranial vena cava close to the right atrium of the heart. The free end was brought sc to a stab wound in the dorsal neck region.The catheter was secured in the body bv a disc of silicone rubber sheeting (1 mm thick, 30 mm in diameter), which was attached to the rubber with 382 Elasomer adhesive (Dow Corning) close to where it entered the jugular vein.Between blood collections the catheters were filled with sterile saline (0.99? sodium chloride solution) containing 100 IU/ml heparin (Liquemin 25.000®, Hoffmann-La Roche, Grenzach). Blood samples were withdrawn before (referred as basal levels) and 20, 30, 40, 80 and 140 min after iv injection of saline, ACTH or LVP. The heparin¬ ized samples were centrifuged immediately and plasma (5.0 ml) was stored at -20°C un...

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“…As shown in table I, the rabbit adrenal cortex is highly responsive to tetracosactide administration [Faulborn et al, 1979;Fenske et al, 1981a, b]. Some variation occurs in the basal glucocorticoid levels in blood between various rabbit strains and with age and sex of the animals [for a review, see Fenske et al, 1981a, b].…”

Section: Resultsmentioning

confidence: 99%

Effect of ACTH on the Composition of the Aqueous Humour of the Rabbit Eye

Stárka¹,

Obenberger²,

Hampl³

1983

Ophthalmic Res

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Tetracosactide (Synacthen, Ciba) increases the concentration of corticosterone in the aqueous humour of the rabbit eye. The maximal increase, about 5-fold of the basal level, occurs with a slight delay of approximately 30 min after the maximally stimulated corticosterone level in blood plasma. Glucocorticoid elevation is paralleled by an increase of protein content and a slight rise of glucose in aqueous. A disruption of the blood-aqueous barrier by ACTH similar to the action of α-melanocyte-stimulating hormone is suggested.

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Effects of an Intravenous Injection of Tetracosactid on Plasma Corticosteroid and Testosterone Levels in Unstressed Male Rabbits

Cited by 25 publications

References 6 publications

Fox Odour Increases Vigilance in European Rabbits: A Study under Semi‐Natural Conditions

Fox Odour Increases Vigilance in European Rabbits: A Study under Semi‐Natural Conditions

Corticotropin- and lysine-vasopressin induced changes of plasma corticosteroids and testosterone in the adult male pig

Effect of ACTH on the Composition of the Aqueous Humour of the Rabbit Eye

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