Effects of anAgaricus blazeiAqueous Extract Pretreatment on Paracetamol-Induced Brain and Liver Injury in Rats

Abstract

The action of an Agaricus blazei aqueous extract pretreatment on paracetamol injury in rats was examined not only in terms of the classical indicators (e.g., levels of hepatic enzymes in the plasma) but also in terms of functional and metabolic parameters (e.g., gluconeogenesis). Considering solely the classical indicators for tissue damage, the results can be regarded as an indication that the A. blazei extract is able to provide a reasonable degree of protection against the paracetamol injury in both the hepatic and brain tissues. The A. blazei pretreatment largely prevented the increased levels of hepatic enzymes in the plasma (ASP, ALT, LDH, and ALP) and practically normalized the TBARS levels in both liver and brain tissues. With respect to the functional and metabolic parameters of the liver, however, the extract provided little or no protection. This includes morphological signs of inflammation and the especially important functional parameter gluconeogenesis, which was impaired by paracetamol. Considering these results and the long list of extracts and substances that are said to have hepatoprotective effects, it would be useful to incorporate evaluations of functional parameters into the experimental protocols of studies aiming to attribute or refute effective hepatoprotective actions to natural products.

Protective Effect of Moringa Peregrina Leaves Extract on Acetaminophen - Induced Liver Toxicity in Albino Rats.

“…On one hand, there is accumulating evidence in harmony with our data, established by Soares et al (2013) and Shull et al (1991), who postulated that exposure of the epithelial cells to H 2 O 2 causes a significant induction in mRNA of GPx, while, insufficient amounts of intracellular GSH, substrate of GPx, might explain why the level of lipid peroxidation in the liver was not affected by GPx overexpression, as well (Lubos et al, 2011).…”

“…The reduction in the activities of CAT and SOD enzymes in APAP rats was attributed to the increased utilization of these enzymes in scavenging and neutralizing the free radicals and lipid peroxides (Singh et al, 2011). The aforementioned data are in harmony with those obtained by El-Banna et al (2013) and Soares et al (2013).…”

Hepatoprotective Effects of Mushrooms

“…Further studies including clinical trials need to be carried out to ascertain the safety of these compounds as good alternatives to conventional drugs in the treatment of liver diseases. Another perspective results from a recent study in which hepatoprotection by an Agaricus blazei extract on the paracetamol-induced injury was investigated [118]. It was found that the extract was quite effective in normalizing several of the classical markers of liver injury such as the release of enzymes into the plasma and the lipid peroxidation levels.…”

Avaliação Da Peroxidação Lipídica No Plasma De Ratos Submetidos À Lesão Tecidual E Tratados Com Hidrogel De Poliamido De Mandioca

“…Os danos oxidativos na membana celular -PL é demonstrado pelos altos níveis de TBARS encontrados em tecidos lesionados (SOARES et al, 2013;GONÇALVES et al, 2015). Essse níveis elevados de TBARS podem ser normalizados com produtos bioativos com potencial antioxidante, antioxidantes fenólicos, que agem como sequestradores de radicais, e, por vezes, agem como quelantes metálicos, atuando tanto na etapa de iniciação e propagação da oxidação, prevenindo a peroxidação lipídica (SOARES et al, 2013).…”

“…Essse níveis elevados de TBARS podem ser normalizados com produtos bioativos com potencial antioxidante, antioxidantes fenólicos, que agem como sequestradores de radicais, e, por vezes, agem como quelantes metálicos, atuando tanto na etapa de iniciação e propagação da oxidação, prevenindo a peroxidação lipídica (SOARES et al, 2013).…”