Protective Effect of Moringa Peregrina Leaves Extract on Acetaminophen - Induced Liver Toxicity in Albino Rats.

Abstract

Background: Acetaminophen is a common antipyretic drug but at overdose can cause severe hepatotoxicity that may further develop into liver failure and hepatic centrilobular necrosis in experimental animals and humans. This study was undertaken to assess the ameliorative role of Moringa peregrina leaves extract against acetaminophen toxicity in rats. Materials and methods: Induction of hepatotoxicity was done by chronic oral administration of acetaminophen (750 mg/kg bwt) for 4 weeks. To study the possible hepatoprotective effect, Moringa peregrina leaves extract (200 mg/kg bwt) or Silymarin (50 mg/kg bwt) was administered orally, for 4 weeks, along with acetaminophen. Results: acetaminophen significantly increased serum liver enzymes and caused oxidative stress, evidenced by significantly increased tissue malondialdehyde, glutathione peroxidase, hepatic DNA fragmentation, and significant decrease of glutathione and antioxidant enzymes in liver, blood and brain. On the other hand, administration of Moringa peregrina leaves extract reversed acetaminophen-related toxic effects through: powerful malondialdehyde suppression, glutathione peroxidase normalization and stimulation of the cellular antioxidants synthesis represented by significant increase of glutathione, catalase and superoxide dismutase in liver, blood and brain, besides, DNA fragmentation was significantly decreased in the liver tissue. Conclusion: acetaminophen induced oxidative damage can be improved by Moringa peregrina leaves extracttreatment, due to its antioxidant potential.

Phytochemical and Antimicrobial Activity Evaluation of The Water Immiscible Solvent Extracts of Moringa

“…According to this data, all the extracts showed positive tests for alkaloids, saponins, and flavonoids. This shows that alkaloids, saponins, and flavonoids are among the main chemical constituents of the [15][16][17][18][19][20][21] .…”

Traditional Uses, Pharmacological Efficacy, and Phytochemistry of Moringa peregrina (Forssk.) Fiori. —A Review

“…The IC 50 value of the leaf extract was 7.1 μg/ml. While the positive control, ascorbic acid showed the IC 50 value of 4.6 μg/ml (Azim et al, 2017 ). The extract of M. peregrina young leaves showed 74.78% of DPPH free radical inhibition (Juhaimi et al, 2017 ).…”

“…In the meantime, the superoxide dismutase, catalase, and glutathione peroxidase activities were reduced significantly in the rats intoxicated with acetaminophen. The superoxide dismutase, catalase and glutathione peroxidase activities enhanced in successful way when the M. peregrina leaf extract was administrated (Azim et al, 2017 ). In literature, triterpenoids have been reported as one of the most important anti-hepatotoxic agents.…”

“…Recently, M. peregrina is gaining more attention due to traditional, nutritional, industrial and medicinal values. As this plant has wide range of medicinal uses, it has been screened for various pharmacological activities in the past few decades (Marwah et al, 2007 ; Soltan and Zaki, 2009 ; Koheil et al, 2011 ; Dehshahri et al, 2012 ; Lalas et al, 2012 ; Al-Owaisi et al, 2014 ; Moustafa et al, 2014 ; Majali et al, 2015 ; Safaeian et al, 2015 ; Ullah et al, 2015 ; Alrayes et al, 2016 ; El-Awady et al, 2016 ; Azim et al, 2017 ; Elabd et al, 2017 ; Saleh et al, 2017 ). Few active molecules were also isolated, identified and reported for various pharmacological activities.…”

Protection efficiency of red radish seeds and roots against paracetamol mediated hepatotoxicity in rats

“…So, the maintenance of a healthy liver is crucial for the overall health of an individual but unfortunately there are various environmental and chemical agents such as chemotherapeutic agents and certain antibiotics which cause injury of the liver cells or hepatotoxicity (2). Although the use of acetaminophen or paracetamol is widespread as antipyretic drug but it is considered to be one of the causes of hepatotoxicity which may further develop into liver failure when overdose used (3). During the metabolism of paracetamol via glucuronidation and sulfation reactions, water-soluble metabolites are produced then excreted by the kidney.…”